Muscle Response to High Intensity Interval Resistance Training (HIIRT)

• Sponsor: University of Padova (Other)
• Overall Status: Completed
• CT.gov ID: NCT04321772
• Collaborator: University of Roma La Sapienza (Other)
• Enrollment: 9
• Location: 1
• Arms: 2
• Actual Duration (Months): 12.3
• Patients Per Site Per Month: 0.7

Study Details

Study Description

Brief Summary

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Condition or Disease	Intervention/Treatment	Phase
Exercise; Resistance Training	Other: High Intensity Interval Resistance Training; Other: Traditional Resistance Training	N/A

Detailed Description

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Study Design

• Study Type: Interventional
• Actual Enrollment: 9 participants
• Allocation: Randomized
• Intervention Model: Crossover Assignment
• Intervention Model Description: Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)
• Masking: Single (Outcomes Assessor)
• Masking Description: The samples analysed were masked by a number without any indication of the arm.
• Primary Purpose: Basic Science
• Official Title: Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training
• Actual Study Start Date: Feb 1, 2012
• Actual Primary Completion Date: May 15, 2012
• Actual Study Completion Date: Feb 10, 2013

Arms and Interventions

Arm	Intervention/Treatment
Experimental: High Intensity Interval Resistance Training (HIIRT) HIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.	Other: High Intensity Interval Resistance Training; Subjects trained one leg with HIIRT
Active Comparator: Traditional Resistance Training (TRT) TRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75" of rest between sets.	Other: Traditional Resistance Training; Subjects trained the other leg with HIIRT

Outcome Measures

Primary Outcome Measures

1. pAMPKThr172 in skeletal muscle tissue measured by immunoblot [24 hours]

   measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy

2. pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot [24 hours]

   measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy

3. pS6Ser235/236 in skeletal muscle tissue measured by immunoblot [24 hours]

   measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy

4. p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot [24 hours]

   measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy

5. ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot [24 hours]

   ACC phosphorylation in skeletal muscle tissue taken by biopsy

6. IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

   IGF-1 mRNA in skeletal muscle tissue taken by biopsy

7. IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hoursn]

   IGF-1ea mRNA in skeletal muscle tissue taken by biopsy

8. MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

   MGF mRNA in skeletal muscle tissue taken by biopsy

9. STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hoursn]

   STARS mRNA in skeletal muscle tissue taken by biopsy

10. Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    Myogenin mRNA in skeletal muscle tissue taken by biopsy

11. IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    IL-6 mRNA in skeletal muscle tissue taken by biopsy

12. PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    PGC1a4 mRNA in skeletal muscle tissue taken by biopsy

13. PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    PGC1a mRNA in skeletal muscle tissue taken by biopsy

14. Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    Atrogin mRNA in skeletal muscle tissue taken by biopsy

15. Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    Beclin mRNA in skeletal muscle tissue taken by biopsy

16. Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) [24 hours]

    Myostatin mRNA in skeletal muscle tissue taken by biopsy

Eligibility Criteria

Criteria

• Ages Eligible for Study: 20 Years to 30 Years
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: Yes

Inclusion Criteria:

• moderate active

• experience with resistance training

Exclusion Criteria:

• history of recent myocardial infarction,

• severe cardiac arrhythmia,

• unstable angina,

• poorly controlled hypertension,

• poorly controlled diabetes mellitus,

• frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Nutrition and Exercise Lab, DSB, University of Padova	Padova		Italy	35131

Sponsors and Collaborators

• University of Padova
• University of Roma La Sapienza

Investigators

• Study Director: Antonio Paoli, MD, University of Padova

Study Documents (Full-Text)

None provided.

More Information

Publications

• Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237.
• Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22.
• Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011.

• Responsible Party: Antonio Paoli, Full Professor, University of Padova
• ClinicalTrials.gov Identifier: NCT04321772
• Other Study ID Numbers: HIIRT12
• First Posted: Mar 25, 2020
• Last Update Posted: Mar 25, 2020
• Last Verified: Mar 1, 2020

• Individual Participant Data (IPD) Sharing Statement: No
• Plan to Share IPD: No
• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No
• Keywords provided by Antonio Paoli, Full Professor, University of Padova: resistance training; recovery; muscle hypertrophy; muscle signalling

Study Results

No Results Posted as of Mar 25, 2020