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Exercise Dose-response and Protein Requirements

Sponsor
University of Toronto (Other)
Overall Status
Completed
CT.gov ID
NCT06138951
Collaborator
(none)
7
Enrollment
1
Location
3
Arms
13.5
Actual Duration (Months)
0.5
Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Study will investigate the impact of training volume on protein metabolism to estimate the impact on daily protein requirements of endurance athletes.

Condition or Disease Intervention/Treatment Phase
  • Other: Endurance exercise
 N/A

Study Design

Study Type:
Interventional
Actual Enrollment :
7 participants
Allocation:
Randomized
Intervention Model:
Crossover Assignment
Masking:
None (Open Label)
Primary Purpose:
Basic Science
Official Title:
Dose Response Effect of Endurance Exercise on Protein Requirements of Endurance Athletes
Actual Study Start Date :
Nov 17, 2017
Actual Primary Completion Date :
Aug 15, 2018
Actual Study Completion Date :
Jan 1, 2019

Arms and Interventions

Arm Intervention/Treatment
Placebo Comparator: Nonexercised rested day

Athlete will not exercise on trial day.

Other: Endurance exercise
On the trial day before commencing the indicator amino acid oxidation metabolic trial, participants will either rest, perform a 10km run on a treadmill, or perform a 20km run on a treadmill.
Experimental: Recovery from 10KM run

Athlete will run 10km on trial day.

Other: Endurance exercise
On the trial day before commencing the indicator amino acid oxidation metabolic trial, participants will either rest, perform a 10km run on a treadmill, or perform a 20km run on a treadmill.
Experimental: Recovery from 20KM run

Athlete will run 20km on trial day.

Other: Endurance exercise
On the trial day before commencing the indicator amino acid oxidation metabolic trial, participants will either rest, perform a 10km run on a treadmill, or perform a 20km run on a treadmill.

Outcome Measures

Primary Outcome Measures

  1. Phenylalanine excretion [8 hours]

    Excretion of 13CO2 in the breath will be used to estimate the impact of exercise on daily protein requirements. This outcome will include the enrichment of 13CO2 in exhaled breath after the ingestion of [13C]phenylalanine tracer and the production of CO2 determined by indirect calorimetry at rest or after exercise.

Secondary Outcome Measures

  1. Phenylalanine flux [8 hours]

    The enrichment of [13C]phenylalanine in the urine will be used to estimate the flux of phenylalanine in the body at rest or after exercise.

  2. Phenylalanine oxidation [8 hours]

    The oxidation of [13C]phenylalanine will be determined by correcting phenylalanine excretion by phenylalanine flux.

Eligibility Criteria

Criteria

Ages Eligible for Study:
18 Years to 40 Years
Sexes Eligible for Study:
Male
Accepts Healthy Volunteers:
Yes
Inclusion Criteria:

Healthy, male, endurance-trained participants who regularly run more than 40 km/week or perform endurance exercise for more than 4.5 hours/week

  • Participants who have a self-reported 5-km run time of ≤23 min

  • Participants who are categorized as at least "very good" based on a study by Shvartz & Reibold (Shvartz 1990), in which VO2peak is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y)

  • Ability to complete the 16-km familiarization run on Session 2

  • Aged 18-40 years old

Exclusion Criteria:
    • Inability to meet health and physical activity guidelines according to the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix 1).

  • Female: hormonal fluctuations associated with the menstrual cycle have been reported to alter protein metabolism during exercise (Moore, Camera, Areta, and Hawley, 2014; Hamadeh, Devries, and Tarnopolsky 2005) and may influence specific amino acid requirements at rest (Kriengsinyos, Wykes, Goonewardene, Ball, and Pencharz 2004); as such, the present study will test the effect of endurance exercise on protein requirements in males only to ensure a stable hormonal environment and to increase the homogeneity of the physiological response. Nevertheless, given that WHO/FAO protein requirements are provided independently of sex and that recommendations for protein intake in athletes generally do not distinguish between the sexes (American Dietetic A, Dietitians of C, American College of Sports, Rodriguez, Di Marco, and Langley,

  1. or that protein requirements have been reported to be lower in endurance trained females than males (Rowland and Wadswortt, 2011), it could also be argued that protein requirements determined by IAAO would be similar between the sexes and/or potential reduced in females. Therefore, to investigate the effect of endurance exercise on protein requirements, we will test males as an indication of the potentially greatest protein requirement between the sexes. (NOTE: we pan to be test females in a future trial based off of the results obtained herein)

  • Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)

  • Regular tobacco use (screened by survey sheet for training log (Appendix 2))

  • Illicit drug use (e.g. growth hormone, testosterone, etc.)

Contacts and Locations

Locations

Site City State Country Postal Code
1 Goldring Centre for High Performance Sport at the University of Toronto Toronto Ontario Canada M5S2C9

Sponsors and Collaborators

  • University of Toronto

Investigators

None specified.

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.
Responsible Party:
Daniel Moore, Associate Professor, University of Toronto
ClinicalTrials.gov Identifier:
NCT06138951
Other Study ID Numbers:
  • DRE
First Posted:
Nov 18, 2023
Last Update Posted:
Nov 18, 2023
Last Verified:
Nov 1, 2023
Individual Participant Data (IPD) Sharing Statement:
No
Plan to Share IPD:
No
Studies a U.S. FDA-regulated Drug Product:
No
Studies a U.S. FDA-regulated Device Product:
No

Study Results

No Results Posted as of Nov 18, 2023