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Expression of miRNa-138 and Cyclin D1 in Oral Mucosa of Patients With Oral Lichen Planus

Sponsor
Cairo University (Other)
Overall Status
Completed
CT.gov ID
NCT02834520
Collaborator
(none)
60
Enrollment
10
Duration (Months)

Study Details

Study Description

Brief Summary

60 individuals were subdivided into 2 groups, 30 patients with oral lichen planus, 30 control individuals. Expression of miRNA-138 and cyclin D1 were evaluated in oral mucosa utilizing Quantitative real-time polymerase chain reaction and immunohistochemistry.

Condition or Disease Intervention/Treatment Phase

    Detailed Description

    60 individuals were subdivided into 3 groups, into 2 groups, 30 patients with oral lichen planus, 30 control individuals having age, gender and periodontal status matched with both patients groups acting as a control group. Relative gene expression of miRNA-138 was estimated using Quantitative real-time polymerase chain reaction technique in oral mucosa of both oral lichen planus patients and control subjects. Sub grouping of oral lichen planus patients were performed into reticular, atrophic and erosive groups (n=10 each). Cyclin D1 gene expression was estimated using Quantitative real-time polymerase chain reaction technique and Cyclin D1 protein level was estimated using immunohistochemistry.

    Study Design

    Study Type:
    Observational
    Actual Enrollment :
    60 participants
    Observational Model:
    Case-Control
    Time Perspective:
    Cross-Sectional
    Official Title:
    Expression of miRNA-138 and Cyclin D1 in Oral Lichen Planus (A Study Utilizing Immunohistochemistry and Real-time Polymerase Chain Reaction)
    Study Start Date :
    May 1, 2015
    Actual Primary Completion Date :
    Dec 1, 2015
    Actual Study Completion Date :
    Mar 1, 2016

    Arms and Interventions

    Arm Intervention/Treatment
    oral lichen planus

    30 patients suffering from reticular, erosive and atrophic oral lichen planus
    control subjects

    30 individuals age, gender and periodontal status matched with oral lichen planus patients and not suffering from any oral mucosal lesions or periodontal disease.

    Outcome Measures

    Primary Outcome Measures

    1. expression of miRNA-138 [1 day]

      expression of miRNA-138 in oral mucosa of patients with oral lichen planus

    Secondary Outcome Measures

    1. gene expression of cyclin D1 [1 day]

      gene expression of cyclin D1 in oral mucosa of patients with oral lichen planus

    2. protein expression of cyclin D1 [1 day]

      protein expression of cyclin D1 in oral mucosa of patients with oral lichen planus

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    45 Years to 65 Years
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes
    Inclusion Criteria:
    • patients with oral lichen planus.
    Exclusion Criteria:

    • any systemic disease other than oral lichen planus.

    • smokers.

    • pregnant females.

    • periodontal diseases.

    Contacts and Locations

    Locations

    No locations specified.

    Sponsors and Collaborators

    • Cairo University

    Investigators

    • Principal Investigator: Noha Ghallab, MD, Associate Professor of periodontology and Oral Medicine faulty of oral an

    Study Documents (Full-Text)

    None provided.

    More Information

    Publications

    None provided.
    Responsible Party:
    Noha Ayman Ghallab, Associate Professor of Oral Medicine and Periodontology, Cairo University
    ClinicalTrials.gov Identifier:
    NCT02834520
    Other Study ID Numbers:
    • MiRNA-138_cyclin D1_OLP
    First Posted:
    Jul 15, 2016
    Last Update Posted:
    Jul 15, 2016
    Last Verified:
    Jul 1, 2016
    Additional relevant MeSH terms:
    Lichen Planus, Oral
    Lichen Planus

    Study Results

    No Results Posted as of Jul 15, 2016