External Nitric Oxide Measurement Through SNO Degradation
Study Details
Study Description
Brief Summary
In this study, we aim to explore the feasibility of a novel, noninvasive SNO assay to acquire physiological SNO quantification from the human ear, forehead, or hands and test this new method of analysis. This study aims to help with the currently cumbersome and invasive procedures used to measure SNOs in the body. The proposed activities do not unnecessarily duplicate previous experiments.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
S-nitrosylation is the covalent attachment of a nitric oxide group to cystine thiol within a protein to form an S-nitrosothiol (SNO); it has diverse regulatory roles in all mammalian cells and thus operates as a fundamental mechanism for cellular signaling and accounts for a large part of nitric oxide activity. Though SNOs are relevant to many biological disciplines like neuronal, muscular, respiratory, and cardiovascular biology, currently there is no way to measure SNOs that is both easy to use in a clinical setting and accurate in detecting low concentrations; detection is challenging due to the labile nature of the molecules. Low or high concentrations of these molecules could be vital indicators of incoming dangerous issues in the body. A system to easily and accurately measure SNOs could prove useful in preventative treatments. Ultra Violet (UV) light can break SNOs and can be used to measure nitric oxide release, but this method is not fully developed and further research is needed on the potential effect for UV to measure nitric oxide release.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Healthy Volunteers Study will enroll 5 healthy volunteers to test the assay |
Device: Alonefire SV003 10W 365nm UV Flashlight
Investigator will use a Nitric Oxide Analyzer while attached to a small suction port on the subject's ear lobe to be tested for Nitric Oxide. After getting the baseline assay, the investigator will shine a low-intensity UV Flashlight (Alonefire SV003 10W 365nm UV Flashlight) at the ear lobe for 10 seconds, repeated three times at 20 second intervals, to determine if there is photobleaching. Then the assay will then be performed on the opposite ear lobe. Fifteen minutes after the third UV flashlight exposure, the investigator will collect air from the subject's ear during photolysis into a 20mL glass syringe (once from each ear). Fifteen minutes after the third UV flashlight exposure, the investigator will collect air from the subject's ear during photolysis into a 20mL glass syringe (once from each ear). The subject and the investigator will wear UV goggles during the experiment.
|
Outcome Measures
Primary Outcome Measures
- Change in Nitric Oxide during UV light exposure [30 minutes]
NO evolved during UV light exposure from the human ear lobe, hand or forehead.
Eligibility Criteria
Criteria
Inclusion Criteria
- Adult males or females age ≥ 1 day and ≤ 75 years at time of enrollment
Exclusion Criteria
-
Subjects meeting the following criteria will be excluded:
-
Smokers.
-
Any chronic skin conditions.
-
Pregnant.
-
Subjects that are unable or unwilling to cooperate with specimen collection.
-
Subjects with diagnosis of any medical condition that in the investigator's opinion would make them unsuitable for study participation.
-
(for subjects ≥ 18) Subjects that lack the capacity to consent for themselves.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Riley Hospital for Children | Indianapolis | Indiana | United States | 46202 |
| 2 | Wells Center for Pediatric Research | Indianapolis | Indiana | United States | 46202 |
Sponsors and Collaborators
- Indiana University
Investigators
- Principal Investigator: Nadzeya Marozkina, PhD, MD, Indiana University School of Medicine
Study Documents (Full-Text)
None provided.More Information
Publications
- Marozkina NV, Gaston B. Nitrogen chemistry and lung physiology. Annu Rev Physiol. 2015;77:431-52. doi: 10.1146/annurev-physiol-021113-170352. Review.
- Marozkina NV, Gaston B. S-Nitrosylation signaling regulates cellular protein interactions. Biochim Biophys Acta. 2012 Jun;1820(6):722-9. doi: 10.1016/j.bbagen.2011.06.017. Epub 2011 Jun 24. Review.
- Palmer LA, Gaston B. S-nitrosothiol assays that avoid the use of iodine. Methods Enzymol. 2008;440:157-76. doi: 10.1016/S0076-6879(07)00809-9. Review.
- Straub AC, Lohman AW, Billaud M, Johnstone SR, Dwyer ST, Lee MY, Bortz PS, Best AK, Columbus L, Gaston B, Isakson BE. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature. 2012 Nov 15;491(7424):473-7. doi: 10.1038/nature11626. Epub 2012 Oct 31.
- 10839