Role of Genexpert in Extra Pulmonary Tuberculosis
Study Details
Study Description
Brief Summary
Diagnosis of extra pulmonary tuberculosis remains especially challenging since the number of Mycobacterium tuberculosis bacilli present in tissues at sites of disease is often low and clinical specimens from deep-seated organs may be difficult to obtain. Histology is time-consuming to undertake and establishing a diagnosis of tuberculosis with high specificity remains difficult. Tissue microscopy after special staining is often negative and when mycobacteria are seen, it is impossible to distinguish Mycobacterium tuberculosis from non tuberculous mycobacterial disease. Reliance on culture, the mainstay of diagnosis, often leads to considerable delays, compromising patient care and outcomes.
Evidence from 138 studies published before 2008 suggested that nucleic acid amplification technologies could not replace conventional mycobacterial tests (microscopy, culture) for diagnosing pulmonary and, especially, extra pulmonary tuberculosis
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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N/A |
Detailed Description
Only a few years later, GeneXpert technology has changed this paradigm, with a recent systematic review showing pooled sensitivity of 88% and pooled specificity of 98% for diagnosis of pulmonary tuberculosis, but evidence (as of March 2012) for using Xpert MTB/RIF for diagnosing extra pulmonary tuberculosis is still comparatively weak Globally, there is still a dearth of studies involving the use of Xpert MTB/RIF in extra pulmonary tuberculosis specimens, and few provide definitive answers. This is due mostly to the studies having small sample sizes across a range of various specimen types and differences in pre-processing methodologies and in input volumes and to studies having been conducted in different populations (adults, children, Human immunodeficiency virus infected).
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Other: tuberculous pleural effusion diagnosis of tuberculous pleural effusion and genitourinary tuberculosis by Genexpert by urine and pus and pleural fluid aspirate |
Diagnostic Test: Genexpert
diagnosis of tuberculous pleural effusion and genitourinary tuberculosis by Genexpert by urine and pus and pleural fluid aspirate
|
Outcome Measures
Primary Outcome Measures
- the percentage of positive patients for extra pulmonary tuberculosis [10 minutes]
Count of tuberculosis bacilli in the specimen
Eligibility Criteria
Criteria
Inclusion Criteria:
- All patients with extra pulmonary tuberculosis
Exclusion Criteria:
-
Severe co morbid diseases.
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pulmonary tuberculosis
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patients refuse to do genexpert or refusing
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Assiut University
Investigators
- Study Chair: Raafat Talett, Assiut University
- Study Chair: Yaser Ahmed, Assiut University
- Study Chair: Ahmed Metwally, Assiut University
Study Documents (Full-Text)
None provided.More Information
Publications
- PTB