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FTO rs9939609 and PPARy rs1801282 Polymorphisms in Mexican Adolescents

Sponsor
Asociación Científica Latina A.C. (Other)
Overall Status
Completed
CT.gov ID
NCT02886013
Collaborator
(none)
71
Enrollment
30.9
Duration (Months)

Study Details

Study Description

Brief Summary

Background and Aims: The presence of the FTO rs9939609 and PPARy rs1801282 polymorphisms suggests changes in energy metabolism; this variation may be responsible for the development of various diseases including obesity. The aim of this study was to identify the presence of these polymorphisms in Mexican adolescents with overweight and obesity at high risk for developing diabetes. Methods and Results: This was a descriptive cross-sectional study, where 71 healthy adolescents (average age of 16) were included. Anthropometric measurements, Body mass index, as well as the determination of glucose, insulin and HOMA index were calculated from all the patients. The FTO rs9939609 and PPARy rs1801282 polymorphisms were determined by real-time PCR.

Condition or Disease Intervention/Treatment Phase

    Detailed Description

    Study Population Students without known disease, between 14 and 18 years from the "Lic. Adolfo Lopez Mateos" Preparatory school of the Autonomous University of the State of Mexico (UAEMex), were invited to participate.

    Anthropometry The body mass index (BMI) was calculated as weight (kg) divided by height (m) squared with conventional stadiometer; dividing the results in normal, overweight and obesity according to the classification of the World Health Organization (WHO), specifically for adolescents by gender and age.

    Laboratory 3 mL of venous blood (Vacutainer tubes) were taken in fasting. The concentration of glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides were determined by enzymatic methods (Randox Laboratories Ltd, County Antrim, UK); whereas leptin and adiponectin concentrations were determined by the immunosorbent assay (ELISA) (Invitrogen MR Frederyck MD USA). A sample remained frozen at -70°C (900 series Thermo Scientific) until DNA extraction.

    Genotyping DNA extraction was performed in the Magna Pure LC 2.0 Instrument (Roche, Germany) using the MagNA Pure LC DNA Isolation Kit 1 (Roche, Germany). After extraction, the DNA was quantified using a NanoPhotometer (Implen GmbH, Germany), reporting concentration (μg/ml) and purity (260/280 absorbance). Genotyping was performed by polymerase chain reaction (PCR) Life express thermal cycler (Bioer, China). The primers and conditions for each polymorphism are listed in Table 1.

    The oligonucleotides were designed using the Primer Quest web tool (Integrated DNA Technologies, Inc., CA, USA) and synthesized at the Synthesis and DNA Sequencing Unit of the National Autonomous University of Mexico (UNAM) Institute of Biotechnology (Cuernavaca, Morelos, Mexico). A search was performed in BLAST to verify the correct hybridization. The final products were visualized in 2% agarose gels, stained with ethidium bromide, and visualized under an UV transilluminator (Gel Logic 212 Pro, Carestream, USA). To confirm the detection of polymorphisms sequencing was performed at the National Institute of Genomic Medicine (INMEGEN), Mexico City.

    Study Design

    Study Type:
    Observational
    Actual Enrollment :
    71 participants
    Observational Model:
    Ecologic or Community
    Time Perspective:
    Prospective
    Official Title:
    FTO rs9939609 and PPARy rs1801282 Polymorphisms in Mexican Adolescents With Overweight and Obesity at High Risk for Developing Diabetes
    Study Start Date :
    Jan 1, 2013
    Actual Primary Completion Date :
    Aug 1, 2014
    Actual Study Completion Date :
    Aug 1, 2015

    Arms and Interventions

    Arm Intervention/Treatment
    Mexican adolescents

    Students without known disease, between 14 and 18 years from the "Lic. Adolfo Lopez Mateos" Preparatory school of the Autonomous University of the State of Mexico (UAEMex).

    Outcome Measures

    Primary Outcome Measures

    1. Prevalence of the FTO rs9939609 and PPARy rs1801282 polymorphisms. [up to 24 months.]

      The aim of this study was to identify the presence of these polymorphisms in Mexican adolescents with overweight and obesity at high risk for developing diabetes.

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    14 Years to 18 Years
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes
    Inclusion Criteria:
    • Students without known disease, between 14 and 18 years from the "Lic. Adolfo Lopez Mateos" Preparatory school of the Autonomous University of the State of Mexico (UAEMex).
    Exclusion Criteria:
    • Those with a history of smoking, pregnancy or diagnosed with a chronic diseases.

    Contacts and Locations

    Locations

    No locations specified.

    Sponsors and Collaborators

    • Asociación Científica Latina A.C.

    Investigators

    • Study Chair: María del Socorro Camarillo Romero, PhD., Autonomous University of the State of Mexico

    Study Documents (Full-Text)

    None provided.

    More Information

    Publications

    None provided.
    Responsible Party:
    Dr. Hugo Mendieta Zerón, Director, Asociación Científica Latina A.C.
    ClinicalTrials.gov Identifier:
    NCT02886013
    Other Study ID Numbers:
    • 2016-03
    First Posted:
    Sep 1, 2016
    Last Update Posted:
    Sep 1, 2016
    Last Verified:
    Aug 1, 2016
    Individual Participant Data (IPD) Sharing Statement:
    No
    Plan to Share IPD:
    No
    Keywords provided by Dr. Hugo Mendieta Zerón, Director, Asociación Científica Latina A.C.
    prevalence
    Additional relevant MeSH terms:
    Metabolic Syndrome
    Insulin Resistance

    Study Results

    No Results Posted as of Sep 1, 2016