A Study of the Function of Hormones Present In Taste Buds

Sponsor

National Institute on Aging (NIA) (NIH)

Overall Status

Completed

CT.gov ID

NCT00233298

Collaborator

(none)

225

Enrollment

Location

115.3

Duration (Months)

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The purpose of this study is to find out whether the hormones in the taste buds are affected by tasting and eating food, and also whether these hormone levels are affected by an increase in body weight or type 2 diabetes.

Condition or Disease	Intervention/Treatment	Phase
Diabetes Obesity Pre-Diabetes Metabolic Syndrome

Detailed Description

Cephalic phase of insulin secretion is regulated by autonomic and endocrine responses to food-related sensory stimulation such as sight, smell, and taste. Human taste perception comprises of at least five distinct qualities: bitterness, saltiness, sourness, sweetness, and umami, the sensation elicited by glutamate, commonly found in protein (meat, fish, and legumes) and flavor enhancer such as monosodium glutamate (MSG).

Both the sweet and umami taste stimuli had been shown to illicit cephalic-phase insulin release in rats. Oral sensory stimulation in human with modified sham feeding (MSF where food is smelled, chewed, but not swallowed) had been shown to enhance insulin release during the cephalic phase, lower postprandial glucose level, and improve glucose tolerance in healthy subjects. The loss of pre-absorptive insulin response has been shown to impair glucose tolerance. Furthermore, patients with type 2 diabetes and their first degree relatives had been shown to have impairment of sweet taste.

Recently, glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) have been found in the taste cells located in the taste buds of mice (unpublished data). These new findings raise several interesting questions of whether strict tasting of food without ingestion may stimulate secretion of GLP-1 and PYY from the taste cells, whether their secretion is involved in the afferent input of the cranial nerves, and whether this secretion is impaired in obesity and in patients with pre-diabetes or type 2 diabetes. We also want to investigate whether different tastants, such as sweet versus umami, and different food contents such as percent fat versus carbohydrate compositions, would elicit different hormonal responses.

Study Design

Study Type:

Observational

Actual Enrollment :

225 participants

Time Perspective:

Prospective

Official Title:

A Study of the Function of Hormones Present In Taste Buds

Study Start Date :

May 26, 2005

Study Completion Date :

Jan 2, 2015

Outcome Measures

Primary Outcome Measures

Whether CLP-1 and PYY are involved in the cephalic phase response during feeding [12 months]
Differences in response among healthy, healthy obese, pre-diabetic or 3 diabetes [12 months]

Eligibility Criteria

Criteria

Ages Eligible for Study:

12 Years to 50 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

INCLUSION CRITERIA:

Males or females age 20 to 50
body weight > 50 kg (110 pounds)
Group A
BMI < 25 kg/m(2)
healthy
Group B
BMI greater than or equal to 30 kg/ m(2)
healthy
Group C
Pre-diabetes
Pre-diabetes is defined as having either impaired fasting glucose (IFG) (fasting plasma glucose (FPG) greater than or equal to100 mg/dl but < 126 mg/dl) and/or impaired glucose tolerance (IGT) (2-hour OGTT glucose greater than or equal to140 mg/dl but < 200 mg/dl).
BMI greater than or equal to 30 kg/m(2)
Group D
Type 2 diabetes (on diet or oral agents management only except for thiazolidinediones)
Type 2 diabetes is defined as FPG 126 mg/dl and/or 2-hour OGTT glucose

200

BMI greater than or equal to 30 kg/m(2)
Screening laboratory evaluations with no significant abnormal results:
comprehensive metabolic panel
complete blood count with differential and platelets
fasting plasma glucose < 100 mg/dl for healthy groups only (Group A and B)
2-hour 75-gram OGTT glucose < 140 mg/dl for healthy groups only (Group A and

Negative pregnancy test for women of child-bearing potential
Able to complete an informed consent

EXCLUSION CRITERIA:

Pregnancy (pregnancy has been shown to be associated with decrease in insulin sensitivity
Group A and B subjects cannot have FPG greater than or equal to 100 mg/dl or 2-hr OGTT greater than or equal to 140 mg/dl
Group D subjects cannot have FPG > 240 mg/dl during the screening visit
Group D subjects cannot have their morning fasting finger-stick glucose > 240 mg/dl during the 5 days (3 days for glucophage) prior to the visit when their oral hypoglycemic agent(s) are discontinued
Subjects with type 2 diabetes on insulin therapy
Hematocrit < 36% for women and < 38% for men
Peanut allergy
Presence of other medical conditions that could affect glucose homeostasis
Use of medications known to impair glucose homeostasis (i.e., amiloride shown to inhibit certain taste responses in hamsters)
History of liver or renal disease
History of gastrointestinal or endocrine disorders except for treated hypo- or hyperthyroidism
Long-term glucocorticoid use (over one month), or other immunosuppressive agents within the past 5 years