Gene Testing to Help in the Diagnosis and Treatment of Childhood Brain Tumors

Sponsor

Children's Oncology Group (Other)

Overall Status

Completed

CT.gov ID

NCT00003096

Collaborator

National Cancer Institute (NCI) (NIH)

Enrollment

Locations

122.9

Duration (Months)

1.4

Patients Per Site

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

RATIONALE: Analyzing the number and structure of genes found in a child's cancer cells may help doctors improve methods of diagnosing and treating children with brain tumors.

PURPOSE: This clinical trial is studying the number and structure of genes in cancer cells of children with brain tumors.

Condition or Disease	Intervention/Treatment	Phase
Brain Tumors Central Nervous System Tumors	Genetic: DNA ploidy analysis Genetic: comparative genomic hybridization Genetic: cytogenetic analysis Genetic: fluorescence in situ hybridization

Detailed Description

OBJECTIVES:

Determine the chromosomal gains and losses by DNA ploidy analysis and comparative genomic hybridization in patients with primitive neuroectodermal tumors or medulloblastomas.
Determine the frequency of specific chromosomal abnormalities, including deletions of chromosomal regions 6, 17, and 22, in these patients.
Perform a statistical analysis to determine possible associations of chromosomal abnormalities and DNA ploidy with patient age, tumor histology, tumor location, extent of disease, and event-free survival.

OUTLINE: DNA ploidy analysis will be performed to determine the overall level of aneuploidy. The results are compared to the comparative genomic hybridization (CGH) analysis, which is used to demonstrate tumor-specific losses or gains, including amplification, of specific chromosomal regions. Tumors are also screened for specific abnormalities by fluorescent in situ hybridization (FISH), which detects chromosomal rearrangements, including balanced translocations, deletions, amplifications, etc. PCR-based microsatellite polymorphism analysis may also be performed.

Primitive neuroectodermal tumors (PNETs) are screened by FISH with a distal 17p13.3 cosmid and a 17q25 cosmid to identify tumors with a 17p deletion. Atypical teratoid/rhabdoid tumors and PNETs without a 17p deletion are screened by FISH with a series of cosmids from 22q11.2. PNETs are also screened by interphase FISH with cosmids from chromosome 6 to identify tumors with deletions.

Patients do not receive the results of the genetic testing and the results do not influence the type or duration of treatment.

PROJECTED ACCRUAL: This study will accrue 360 specimens.

Study Design

Study Type:

Observational

Actual Enrollment :

88 participants

Official Title:

Molecular Biology of Pediatric Brain Tumors

Study Start Date :

Dec 1, 1996

Actual Primary Completion Date :

Mar 1, 2006

Actual Study Completion Date :

Mar 1, 2007

Outcome Measures

Primary Outcome Measures

Frequency of specific chromosomal gains, losses and rearrangements in a series of infratentorial and supratentorial PNETS []
Estimate the frequency of specific chromosomal gains, losses and rearrangements in a series of infratentorial and supratentorial PNETS diagnosed in children

Eligibility Criteria

Criteria

Ages Eligible for Study:

N/A to 20 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

DISEASE CHARACTERISTICS:

Histologically confirmed primary CNS malignancy consistent with primitive neuroectodermal tumor, medulloblastoma, or atypical teratoid/rhabdoid tumor
Must be entered on CCG-9921, CCG-9931, CCG-A9961, CCG-99703 or other front-line studies developed from CCG-90024 or CCG-90025
Retrospective specimens also obtained from CCG-921, CCG-923, CCG-9892, CCG-9921, and CCG-9931

PATIENT CHARACTERISTICS:

Age:

Under 21

Performance status:

See Disease Characteristics

Life expectancy:

See Disease Characteristics

Hematopoietic:

See Disease Characteristics

Hepatic:

See Disease Characteristics

Renal:

See Disease Characteristics

PRIOR CONCURRENT THERAPY:

Biologic therapy:

Not specified

Chemotherapy:

Not specified

Endocrine therapy

Not specified

Radiotherapy

No prior radiotherapy

Surgery

Not specified

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Phoenix Children's Hospital	Phoenix	Arizona	United States	85016-7710
2	Southern California Permanente Medical Group	Downey	California	United States	90027
3	Loma Linda University Cancer Institute at Loma Linda University Medical Center	Loma Linda	California	United States	92354
4	Jonathan Jaques Children's Cancer Center at Miller Children's Hospital	Long Beach	California	United States	90801-1428
5	Samuel Oschin Comprehensive Cancer Institute at Cedars-Sinai Medical Center	Los Angeles	California	United States	90048-1865
6	Jonsson Comprehensive Cancer Center at UCLA	Los Angeles	California	United States	90095-1781
7	Children's Hospital Central California	Madera	California	United States	93638-8762
8	Children's Hospital of Orange County	Orange	California	United States	92868
9	Santa Barbara Cottage Children's Hospital	Santa Barbara	California	United States	93105
10	Children's Hospital Center for Cancer and Blood Disorders	Aurora	Colorado	United States	80045
11	Carole and Ray Neag Comprehensive Cancer Center at the University of Connecticut Health Center	Farmington	Connecticut	United States	06360-2875
12	Alfred I. duPont Hospital for Children	Wilmington	Delaware	United States	19803
13	Lombardi Comprehensive Cancer Center at Georgetown University Medical Center	Washington	District of Columbia	United States	20007
14	Children's National Medical Center	Washington	District of Columbia	United States	20010-2970
15	AFLAC Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta - Egleston Campus	Atlanta	Georgia	United States	30322
16	Curtis and Elizabeth Anderson Cancer Institute at Memorial Health University Medical Center	Savannah	Georgia	United States	31403-3089
17	Advocate Lutheran General Cancer Care Center	Park Ridge	Illinois	United States	60068-1174
18	Swedish-American Regional Cancer Center	Rockford	Illinois	United States	61104-2315
19	Simmons Cooper Cancer Institute	Springfield	Illinois	United States	62794-9677
20	St. Vincent Indianapolis Hospital	Indianapolis	Indiana	United States	46260
21	Blank Children's Hospital	Des Moines	Iowa	United States	50309
22	Holden Comprehensive Cancer Center at University of Iowa	Iowa City	Iowa	United States	52242-1002
23	Kosair Children's Hospital	Louisville	Kentucky	United States	40232
24	Butterworth Hospital at Spectrum Health	Grand Rapids	Michigan	United States	49503-2560
25	CCOP - Kalamazoo	Kalamazoo	Michigan	United States	49007-5381
26	Breslin Cancer Center at Ingham Regional Medical Center	Lansing	Michigan	United States	48910
27	Children's Hospitals and Clinics of Minnesota - Minneapolis	Minneapolis	Minnesota	United States	55404
28	Masonic Cancer Center at University of Minnesota	Minneapolis	Minnesota	United States	55455
29	Children's Hospitals and Clinics of Minnesota - St. Paul	St. Paul	Minnesota	United States	55102-2392
30	Children's Mercy Hospital	Kansas City	Missouri	United States	64108
31	CCOP - Nevada Cancer Research Foundation	Las Vegas	Nevada	United States	89109-2306
32	Monmouth Medical Center	Long Branch	New Jersey	United States	07740-6395
33	Carol G. Simon Cancer Center at Morristown Memorial Hospital	Morristown	New Jersey	United States	07962
34	Overlook Hospital	Morristown	New Jersey	United States	07962
35	Newark Beth Israel Medical Center	Newark	New Jersey	United States	07112
36	Lineberger Comprehensive Cancer Center at University of North Carolina - Chapel Hill	Chapel Hill	North Carolina	United States	27599-7295
37	CCOP - MeritCare Hospital	Fargo	North Dakota	United States	58122
38	Cincinnati Children's Hospital Medical Center	Cincinnati	Ohio	United States	45229-3039
39	Rainbow Babies and Children's Hospital	Cleveland	Ohio	United States	44106-5000
40	Cleveland Clinic Taussig Cancer Center	Cleveland	Ohio	United States	44195
41	Nationwide Children's Hospital	Columbus	Ohio	United States	43205-2696
42	Dayton Children's - Dayton	Dayton	Ohio	United States	45404-1815
43	Tod Children's Hospital	Youngstown	Ohio	United States	44501
44	Penn State Children's Hospital	Hershey	Pennsylvania	United States	17033-0850
45	Children's Hospital of Philadelphia	Philadelphia	Pennsylvania	United States	19104-9786
46	Children's Hospital of Pittsburgh of UPMC	Pittsburgh	Pennsylvania	United States	15213
47	Avera Cancer Institute	Sioux Falls	South Dakota	United States	57105
48	East Tennessee Children's Hospital	Knoxville	Tennessee	United States	37901
49	Texas Tech University Health Sciences Center School of Medicine - Amarillo	Amarillo	Texas	United States	79106
50	Dell Children's Medical Center of Central Texas	Austin	Texas	United States	78723
51	Methodist Children's Hospital of South Texas	San Antonio	Texas	United States	78229-3993
52	Primary Children's Medical Center	Salt Lake City	Utah	United States	84113-1100
53	Deaconess Medical Center	Spokane	Washington	United States	99210-0248
54	Gundersen Lutheran Center for Cancer and Blood	La Crosse	Wisconsin	United States	54601
55	University of Wisconsin Paul P. Carbone Comprehensive Cancer Center	Madison	Wisconsin	United States	53792-6164
56	Marshfield Clinic - Marshfield Center	Marshfield	Wisconsin	United States	54449
57	Princess Margaret Hospital for Children	Perth	Western Australia	Australia	6001
58	Children's & Women's Hospital of British Columbia	Vancouver	British Columbia	Canada	V6H 3V4
59	IWK Health Centre	Halifax	Nova Scotia	Canada	B3J 3G9
60	Allan Blair Cancer Centre at Pasqua Hospital	Regina	Saskatchewan	Canada	S4T 7T1
61	Saskatoon Cancer Centre at the University of Saskatchewan	Saskatoon	Saskatchewan	Canada	S7N 4H4