IL-39 & IL-35 Gingival Crevicular Fluid Levels in Diabetic Patients With Generalized Periodontitis
Study Details
Study Description
Brief Summary
The sudy was aiming to assess and compare GCF levels of IL-39 & IL-35 in gingival crevicular fluid and to correlate the relationship between different patient groups before and after non-surgical therapy.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
Methodology:
Group I (Periodontitis Group): 19 patients suffering from generalized periodontitis with T2DM age range, 35-50 years.
Group II (Periodontitis Group): 19 patients suffering from generalized periodontitis & systemically healthy, age range: 35-50 years.
Group III (Control group): 19 Periodontally and systemically healthy volunteers who served as control subjects age range: 30-45 years.
Periodontitis participants will be collected from the outpatient clinic of oral medicine and periodontology departments in Fayoum, Beni-Swief and Ahram-Candian universities. The periodontitis included subjects will be diagnosed according to the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions (Tonetti, MS, Greenwell, H, Kornman, KS. Staging and grading of periodontitis: framework and proposal of a new classification and case definition. J Periodontol.; 89( Suppl 1): S159- S172, 2018.)
Diabetic patients will be enrolled in this study according to the criteria of American Diabetes Association. (American Diabetes Association. (2) Classification and diagnosis of diabetes. Diabetes Care 38: S8-S16, 2015.)
The control group will be selected from healthy subjects who attended the restorative dental clinic and has apparently clinically healthy gingiva.
Micropipettes will be used for collection of GCF samples for control group and periodontitis patients before & after periodontal treatment.
A complete mouth non-surgical periodontal therapy will be carried out for both periodontitis groups
The levels of IL-35 in the GCF samples were assessed using an ELISA kit following the guidelines by the manufacturer.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Active Comparator: Systemically healthy periodontitis Non Surgical Periodontal therapy ( Scaling & Root planning) |
Procedure: Scaling & root planning
Non Surgical periodontal therapy
|
| Active Comparator: Diabetic & Periodontitis Non surgical periodontal therapy ( scaling & root planning) |
Procedure: Scaling & root planning
Non Surgical periodontal therapy
|
| No Intervention: Control Healthy group with neither Diabetes nor Periodontitis |
Outcome Measures
Primary Outcome Measures
- GCF levels of IL-39 & IL35 [3 months]
Gingival crevicular fluid levels of IL-39 & IL-35 in healthy & diabetic patients with periodontitis
- Effect of SRP on GCF levels of IL-39 & IL35 [3 MONTHS]
Gingival crevicular fluid levels of IL-39 & IL-35 in healthy & diabetic patients with periodontitis after Scaling & root planning
Eligibility Criteria
Criteria
Inclusion Criteria Stage III Grade C Generalized periodontitis with diabetes Stage III grade B Generalized periodontitis in systemic healthy subjects
Exclusion Criteria:
-
Patients treated with any type of medications and/ or antibiotics during the past 3 months,
-
Pregnancy
-
Lactation
-
Receiving professional periodontal treatment during the past 6 months
-
Current or former smokers
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Sandy hassan | Cairo | Virginia | Egypt | 22039 |
| 2 | Sandy hassan shaaban | Cairo | Egypt |
Sponsors and Collaborators
- Fayoum University
Investigators
- Principal Investigator: sandy Hassan, ass.prof, associate professor
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- #REC-FDBSU/06102022-02/HS