GPx Activity in Subjects With Aortic Stenosis Undergoing TAVR
Study Details
Study Description
Brief Summary
The aim of this project is to investigate the association of glutathione peroxidase (GPx) and severe aortic stenosis (AS), as well as the impact of transcatheter aortic valve replacement (TAVR) on GPx activity post-procedure. The burden of oxidative stress will be determined by the measurement of GPx, superoxide dismutase (SOD) and lipoprotein A (Lp(a)). We hypothesize GPx activity is reduced in participants with severe AS vs control groups and GPx activity is to increase after TAVR is performed.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Patients with severe aortic stenosis undergoing TAVR GPx activity levels will be measured on the day of TAVR procedure, the day of discharge, 1 month, and 6 months after the procedure |
Diagnostic Test: Blood draw to measure GPx activity
Routine blood draw to measure levels of blood markers.
Diagnostic Test: Blood draw to measure SOD activity
Routine blood draw to measure levels of blood markers.
Diagnostic Test: Blood draw to measure Lp(a) levels
Routine blood draw to measure levels of blood markers.
|
| Patients without aortic stenosis GPx activity levels will be measured on day of recruitment |
Diagnostic Test: Blood draw to measure GPx activity
Routine blood draw to measure levels of blood markers.
Diagnostic Test: Blood draw to measure SOD activity
Routine blood draw to measure levels of blood markers.
Diagnostic Test: Blood draw to measure Lp(a) levels
Routine blood draw to measure levels of blood markers.
|
Outcome Measures
Primary Outcome Measures
- Level of GPx in blood samples [Baseline up to 6 months]
Glutathione peroxidase activity levels measured from whole blood in U/L units in participants with severe aortic stenosis after transcatheter valve replacement compared with the matched population
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Age >18 years
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Patients with severe AS including participants with low flow, low gradient severe AS and paradoxical severe AS
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Patients meeting criteria for TAVR procedure
Exclusion Criteria:
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Previous aortic valve replacement (surgical or TAVR)
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Participants undergoing chemotherapy
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End stage liver disease/cirrhosis (liver produces GSH)
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | University of Florida | Jacksonville | Florida | United States | 32209 |
Sponsors and Collaborators
- University of Florida
Investigators
- Principal Investigator: Chinelo Udemgba, MD, University of Florida
Study Documents (Full-Text)
None provided.More Information
Publications
- Arsenault BJ, Boekholdt SM, Dubé MP, Rhéaume E, Wareham NJ, Khaw KT, Sandhu MS, Tardif JC. Lipoprotein(a) levels, genotype, and incident aortic valve stenosis: a prospective Mendelian randomization study and replication in a case-control cohort. Circ Cardiovasc Genet. 2014 Jun;7(3):304-10. doi: 10.1161/CIRCGENETICS.113.000400. Epub 2014 Apr 5.
- Blankenberg S, Rupprecht HJ, Bickel C, Torzewski M, Hafner G, Tiret L, Smieja M, Cambien F, Meyer J, Lackner KJ; AtheroGene Investigators. Glutathione peroxidase 1 activity and cardiovascular events in patients with coronary artery disease. N Engl J Med. 2003 Oct 23;349(17):1605-13.
- Bolzán AD, Bianchi MS, Bianchi NO. Superoxide dismutase, catalase and glutathione peroxidase activities in human blood: influence of sex, age and cigarette smoking. Clin Biochem. 1997 Aug;30(6):449-54.
- Mahmaljy H, Tawney A, Young M. Transcatheter Aortic Valve Replacement (TAVR/TAVI, Percutaneous Replacement) [Updated 2019 Dec 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2020 Jan-.
- Memet C, Gerede DM, Ozenci M, Akbulut IM, Acibuca A, Kiliçkap M, Erol C. Evaluation of the Role of Oxidative Stress in Degenerative Aortic Stenosis. J Heart Valve Dis. 2015 Jul;24(4):445-50.
- Nimse, S. B. (2015). Free radicals, natural antioxidants, and their reaction mechanism. Royal Society of Chemistry, 27986-28006.
- Otto CM. Aortic stenosis and hyperlipidemia: establishing a cause-effect relationship. Am Heart J. 2004 May;147(5):761-3.
- Redfors, B. A. (2017). Biomarkers in Aortic Stenosis: A systematic Review. Structural Heart. The Journal of the Heart Team, 1-13.
- Zheng KH, Tsimikas S, Pawade T, Kroon J, Jenkins WSA, Doris MK, White AC, Timmers NKLM, Hjortnaes J, Rogers MA, Aikawa E, Arsenault BJ, Witztum JL, Newby DE, Koschinsky ML, Fayad ZA, Stroes ESG, Boekholdt SM, Dweck MR. Lipoprotein(a) and Oxidized Phospholipids Promote Valve Calcification in Patients With Aortic Stenosis. J Am Coll Cardiol. 2019 May 7;73(17):2150-2162. doi: 10.1016/j.jacc.2019.01.070.
- IRB201902486