Hand Eczema in the Health Care Sector
Study Details
Study Description
Brief Summary
This project aims to investigate the prevalence of bacterial colonization and associated factors in health care workers with hand eczema with focus on Staphylococcus aureus.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Two groups will be examined in this study: health care workers with HE and matched controls invited through a questionnaire study which will be sent to approximately 4000 health care workers in Capital Region. Samples from health care workers with and without hand eczema will be collected after obtaining their written informed consent. Collection of samples: Swabs will be rubbed on the skin for 30 seconds on lesional and non-lesional skin on the dominating hand and from the nostrils. The swabs will immediately be stored in -80 C° (research biobank) for subsequent analysis at Statens Serum Institute.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Health care workers with hand eczema Swabs will be taken from eczema lesions on dominating hand (if possible) and nostril |
Other: eSwabs
from skin
|
| controls (health care workers without hand eczema) swabs will be taken from healthy skin on dominating hand and nostril |
Other: eSwabs
from skin
|
Outcome Measures
Primary Outcome Measures
- Staphyloccous aureus presence [Baseline, cross-sectional]
Prevalence of S. aureus colonization on hands and in nose in two groups of health care workers. The swab samples will be assessed by culturing on selective S. aureus plates (chromID S. aureus; bioMerieux, Marcy l'Etoile, France) and incubated overnight at 37 °C. DNA will be purified from S. aureus isolates using the Qiagen DNeasy Blood and Tissue Purification Kit (Qiagen, Hilden, Germany).
Secondary Outcome Measures
- CC-types and spa-typing [Baseline, cross-sectional]
clonal complex (CC) type and spa-types are characterizing the S. aureus. Will be correlated to departments and professions of health care workers. The S. aureus will be further characterized by spa typing, which will be performed by polymerase chain reaction and Sanger sequencing of the product. The respective CC types will be assigned from the spa type using eBURST (http://eburst.mlst.net).
- Hand Eczema Severity Index (HECSI) [Baseline, cross-sectional]
Validated severity measurement tool for hand eczema performed by the investigator. Severity will be correlated to S. aureus colonization and CC types
Eligibility Criteria
Criteria
Inclusion Criteria:
- The diagnosis of hand eczema (for group with hand eczema)
Exclusion Criteria:
- Other skin diseases (in both groups)
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Yasemin Topal Yüksel | Copenhagen | Kbh Nv | Denmark | 2400 |
Sponsors and Collaborators
- University Hospital Bispebjerg and Frederiksberg
- Statens Serum Institut
Investigators
- Principal Investigator: Yasemin T. Yüksel, MD, University of Copenhagen Bispebjerg and Frederiksberg Hospital
- Study Director: Tove Agner, MD, PhD, University of Copenhagen Bispebjerg and Frederiksberg Hospital
Study Documents (Full-Text)
None provided.More Information
Publications
- Brans R, Kolomanski K, Mentzel F, Vollmer U, Kaup O, John SM. Colonisation with methicillin-resistant Staphylococcus aureus and associated factors among nurses with occupational skin diseases. Occup Environ Med. 2016 Oct;73(10):670-5. doi: 10.1136/oemed-2016-103632. Epub 2016 Jun 29.
- Clausen ML, Edslev SM, Nørreslet LB, Sørensen JA, Andersen PS, Agner T. Temporal variation of Staphylococcus aureus clonal complexes in atopic dermatitis: a follow-up study. Br J Dermatol. 2019 Jan;180(1):181-186. doi: 10.1111/bjd.17033. Epub 2018 Oct 10.
- Haslund P, Bangsgaard N, Jarløv JO, Skov L, Skov R, Agner T. Staphylococcus aureus and hand eczema severity. Br J Dermatol. 2009 Oct;161(4):772-7. doi: 10.1111/j.1365-2133.2009.09353.x. Epub 2009 Jul 3.
- Held E, Skoet R, Johansen JD, Agner T. The hand eczema severity index (HECSI): a scoring system for clinical assessment of hand eczema. A study of inter- and intraobserver reliability. Br J Dermatol. 2005 Feb;152(2):302-7.
- Mernelius S, Carlsson E, Henricson J, Löfgren S, Lindgren PE, Ehricht R, Monecke S, Matussek A, Anderson CD. Staphylococcus aureus colonization related to severity of hand eczema. Eur J Clin Microbiol Infect Dis. 2016 Aug;35(8):1355-61. doi: 10.1007/s10096-016-2672-2. Epub 2016 May 19.
- Pittet D, Allegranzi B, Sax H, Dharan S, Pessoa-Silva CL, Donaldson L, Boyce JM; WHO Global Patient Safety Challenge, World Alliance for Patient Safety. Evidence-based model for hand transmission during patient care and the role of improved practices. Lancet Infect Dis. 2006 Oct;6(10):641-52. Review.
- H20007169