2d-BR: Two-day Bed Rest, Insulin Sensitivity and Muscle Protein Synthesis

Sponsor

University of Exeter (Other)

Overall Status

Withdrawn

CT.gov ID

NCT03877822

Collaborator

(none)

Enrollment

Arm

Study Details

Study Description

Brief Summary

The impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

Condition or Disease	Intervention/Treatment	Phase
Healthy	Behavioral: Habitual activity Behavioral: Bed rest	N/A

Detailed Description

To study the mechanisms underlying inactivity-induced muscle atrophy, the impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

Study Design

Study Type:

Interventional

Actual Enrollment :

0 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Intervention Model Description:

Participants will firstly undergo 2 days of habitual physical activity, followed by 2 days of bed restParticipants will firstly undergo 2 days of habitual physical activity, followed by 2 days of bed rest

Masking:

None (Open Label)

Primary Purpose:

Basic Science

Official Title:

The Impact of Two Days of Bed Rest Versus Two Days of Habitual Activity on Insulin Sensitivity and Cumulative Muscle Protein Synthesis in Healthy Young Males

Actual Study Start Date :

Sep 1, 2021

Actual Primary Completion Date :

Sep 1, 2021

Actual Study Completion Date :

Sep 1, 2021

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Habitual activity and 2 days bed rest Participants will undergo 2 days of habitual activity and 2 days bed rest	Behavioral: Habitual activity Two days of habitual physical activity, under normal free-living conditions Behavioral: Bed rest Two days of bed rest, to induce whole-body physical inactivity

Arm

Intervention/Treatment

Experimental: Habitual activity and 2 days bed rest

Participants will undergo 2 days of habitual activity and 2 days bed rest

Behavioral: Habitual activity

Two days of habitual physical activity, under normal free-living conditions

Behavioral: Bed rest

Two days of bed rest, to induce whole-body physical inactivity

Outcome Measures

Primary Outcome Measures

Change in whole-body insulin sensitivity [During 2 days of habitual physical activity and 2 days of bed rest. Blood glucose and insulin are monitored for 2 hours following ingestion of a standardized meal]
Change in whole-body insulin sensitivity via meal tolerance test

Secondary Outcome Measures

Change in cumulative muscle protein synthesis [During 2 days of habitual physical activity and 2 days of bed rest]
Cumulative muscle protein synthesis via deuterium oxide ingestion. The incorporation of deuterated alanine in muscle is expressed relative to body water enrichments, to obtain muscle protein synthesis in %/day
Change in continuous glucose [Continuous glucose, during the 2 days of habitual activity and 2 days of bed rest]
Change in continuous glucose via continuous glucose monitoring.
Change in muscle volume [Immediately before and immediately after the 2-day bed rest period]
Change in muscle volume via MRI

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 40 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

Male
Aged 18-40

Exclusion Criteria:

Smoker
Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
Any diagnosed cardiovascular disease (e.g. deep vein thrombosis or hypertension)
Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism).
A personal or family history of thrombosis, epilepsy, seizures or schizophrenia.
Any known disorders in muscle metabolism
Regular use of nutritional supplements (i.e. ingestion more than twice per week during the last month)
Metallic implants (including heart pacemaker, cochlear implants, medication pumps, surgical clips, plates or screws).
Allergy to lidocaine
Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture)

Contacts and Locations

Locations

No locations specified.

Sponsors and Collaborators

University of Exeter

Investigators

Principal Investigator: Marlou L Dirks, PhD, University of Exeter

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

University of Exeter

ClinicalTrials.gov Identifier:

NCT03877822

Other Study ID Numbers:

190206/B/05

First Posted:

Mar 18, 2019

Last Update Posted:

Nov 17, 2021

Last Verified:

Nov 1, 2021

Studies a U.S. FDA-regulated Drug Product:

Studies a U.S. FDA-regulated Device Product:

Additional relevant MeSH terms:

Insulin Resistance

Study Results

No Results Posted as of Nov 17, 2021