Effect of Broccoli Sprout on Blood Levels of Sulforaphane to Reduce Responsiveness of Immune System

Sponsor

Imperial College London (Other)

Overall Status

Completed

CT.gov ID

NCT01357070

Collaborator

(none)

Enrollment

Location

Arms

32.1

Duration (Months)

0.2

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The investigators suggest that inducing anti-oxidant enzymes indirectly may be an effective means of providing vascular protection.

Sulforaphane, a naturally occurring compound found in green vegetables (including broccoli, brussel sprouts and cauliflower) is able to protect against cell inflammatory stress by inducing a number of anti-oxidant molecules. Targeted studies on the consumption of broccoli and related vegetables have been shown to be associated with reduced risk of coronary artery disease.

In the present study the investigators want to test whether the consumption of a "broccoli smoothie" containing sulforaphane can protect white blood cells from becoming activated in the presence of an experimental stress and how long this protective effect lasts for. To do this, the investigators will be analysing inflammatory changes in blood samples taken at different times during the study.

Condition or Disease	Intervention/Treatment	Phase
Healthy Volunteers	Dietary Supplement: Brocco-sprout homogenate Dietary Supplement: Alfalfa sprout homogenate	N/A

Detailed Description

Atherosclerosis is the disease process that leads to hardening of the arteries which causes them to become narrowed. This occurs because fat is first deposited on the inside walls of the arteries, then becomes hardened by fibrous tissue and calcium deposition forming a semi-hardened accumulation of material known as plaque. As this plaque grows, it narrows the channel within the artery and causes a reduction in blood and oxygen supply to the affected organ - such as the heart and brain. The investigators now understand that this process is caused by inflammation and activation of the immune cells over a period of time. It has been proposed that targeting these immune cells and reducing the levels of immune activation can protect against cardiovascular diseases.

Previously it has been observed that consumption of fruit and vegetables rich in anti-oxidants can confer a protective effect against cardiovascular diseases. The use of anti-oxidants experimentally has shown protective benefits against activation.

Study Design

Study Type:

Interventional

Actual Enrollment :

6 participants

Allocation:

Randomized

Intervention Model:

Crossover Assignment

Masking:

Double (Participant, Investigator)

Primary Purpose:

Basic Science

Official Title:

Does Broccoli Sprout Consumption Result in Plasma Sulforaphane Levels That Can Attenuate Leukocyte Activation Ex-vivo in Healthy Human Volunteers?

Study Start Date :

May 1, 2011

Actual Primary Completion Date :

Jan 1, 2014

Actual Study Completion Date :

Jan 1, 2014

Arms and Interventions

Arm	Intervention/Treatment
Active Comparator: Brocco-sprout homogenate	Dietary Supplement: Brocco-sprout homogenate Participants will be asked to orally consume on three consecutive days a brocco-sprout homogenate (70g dry weight).
Sham Comparator: Alfalfa sprout homogenate	Dietary Supplement: Alfalfa sprout homogenate Participants will be asked to orally consume on three consecutive days an alfalfa sprout homogenate.

Arm

Intervention/Treatment

Active Comparator: Brocco-sprout homogenate

Dietary Supplement: Brocco-sprout homogenate

Participants will be asked to orally consume on three consecutive days a brocco-sprout homogenate (70g dry weight).

Sham Comparator: Alfalfa sprout homogenate

Dietary Supplement: Alfalfa sprout homogenate

Participants will be asked to orally consume on three consecutive days an alfalfa sprout homogenate.

Outcome Measures

Primary Outcome Measures

Resistance of leucocytes to inflammatory activation following an experimental stress [24 hours after consumption of the third homogenate]
Participants will consume a brocco-sprout homogenate. Blood will be sampled for intracellular markers of leukocyte stress after ex-vivo stimulus with Phorbol ester / Tumour Necrosis Factor alpha. The following parameters will be recorded. Reactive oxygen species detection using aminophenylfluoresceine dye p38 MAP kinase induction NF-kB induction

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

Age 18+ years
Able to commit for the two week duration of the trial

Exclusion Criteria:

Age <18 years
Pregnancy
Vegetarian
History of allergy
Current smoker or smoking cessation within the last 3 months
Current use of inhaled, topical or systemic corticosteroids or within the last 2 weeks
Current use of non-steroidal anti-inflammatory use or within the last 1 week
Current use of nutritional or multivitamin supplements
Current participation in any other Randomised controlled trial

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Cardiovascular Medicine Research Unit, Hammersmith Hospital	London		United Kingdom	W12 0NN

Sponsors and Collaborators

Imperial College London

Investigators

Principal Investigator: Paul C Evans, BSc MSc PhD, Imperial College London

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

Imperial College London

ClinicalTrials.gov Identifier:

NCT01357070

Other Study ID Numbers:

2-bao

First Posted:

May 20, 2011

Last Update Posted:

Jul 8, 2019

Last Verified:

Jul 1, 2019

Individual Participant Data (IPD) Sharing Statement:

Plan to Share IPD:

Studies a U.S. FDA-regulated Drug Product:

Studies a U.S. FDA-regulated Device Product:

Keywords provided by Imperial College London

white blood cell

broccoli

P38 Map Kinase

Study Results

No Results Posted as of Jul 8, 2019