The Role of Ursodeoxycholic Acid in Treatment of Gallstones in Hemolytic Disorders

Sponsor

Shaare Zedek Medical Center (Other)

Overall Status

Terminated

CT.gov ID

NCT02472509

Collaborator

(none)

Enrollment

Location

Arm

33.3

Duration (Months)

0.1

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

It is well established that hemolytic diseases predispose patients to the development of pigment gallstones. Gallstones are noted in at least 5% of children under the age of 10 years, increasing to 40-50% in the second to fifth decades. The co-inheritance of Gilbert's syndrome increases the risk of cholelithiasis four to five-fold.

In patients with chronic hemolysis, total bile lipid concentration is decreased and the total bilirubin to total lipid ratio is increased. This suggests that the conjugating capacity of hepatocytes is surpassed by the excessive amount of bilirubin resulting from hemolysis. Increased bilirubin monoconjugate and unconjugated bilirubin can precipitate in bile and form complexes with inorganic ions, mostly calcium, and develop into stones.

Patients with hemolytic disorders can also develop biliary sludge, a suspension of precipitated particulate matter in bile dispersed in a viscous, mucin-rich liquid phase . The chemical composition of the precipitates correlates well with the composition of the associated stone and sludge often stands as a harbinger of future stone development.

There is strong data suggesting a benefit in treating cholelithiasis with UDCA and also in preventing gallstone development in various high risk scenarios.

There are several proposed mechanisms for the positive effect of UDCA in primary prevention of pigment stones. Mucoglycoproteins are present in significant amounts in black pigment stones and contribute to the matrix of gallstones. UDCA suppresses the secretion of protein and decreases the levels of various proteins in bile . It has also been suggested that increased colonic bile salt may solubilize unconjugated bilirubin and may prevent calcium complexing.

There is no published data at present on the role of UDCA in prevention and treatment of cholelithiasis in hemolytic diseases. The investigators hypothesise that UDCA can be of benefit to patients with hemolytic disorders in the primary prevention of pigment stones, possible resolution of biliary sludge and existent stones, and reduction of symptomatic episodes of cholelithiasis.

Condition or Disease	Intervention/Treatment	Phase
Hemolytic Disorders Gallstones	Drug: Ursodeoxycholic Acid	Phase 4

Detailed Description

The research protocol extends for up to 12 months, during which the participant will attend 3 clinic visits at Shaare Zedek Medical Centre; one at study enrollment, one at 6 months and one at 12 months.

All patients identified will have their medical records reviewed for previous clinical, biochemical or sonographic evidence of gallstones.

Patients will undergo a baseline abdominal ultrasound to assess for biliary sludge, gallstones, or evidence of previous cholecystitis (eg. Thickened gallbladder wall).

All patients will also have blood tested for liver biochemistry, complete blood count, hemolytic screen and fasting lipids.

Participants will be commenced on UDCA 15mg/kg/day (maximum dose 900mg/day) in 2-3 divided doses for 12 months.

Patients who are unable to tolerate tablet medication will be started on UDCA syrup at the same dose.

Each of the in-house visits will include:

An explicit history taking and review of patient's notes, including review of symptoms, blood tests and previous ultrasounds, other medical conditions and medications.
Physical examination - including measuring splenic size, assessing for right upper quadrant abdominal tenderness
Blood tests
Hemoglobin, WBC
Liver biochemistry: ALT, AST, GGT, ALP (Alkaline Phosphatase), Bilirubin (conjugated and unconjugated), Albumin
Hemolytic screen: LDH, haptoglobin, reticulocytes
Fasting lipids: LDL, HDL, Triglycerides

Repeat abdominal ultrasound will be performed at 12 months. The US will specifically relate to the following features in comparison to the baseline US:

Presence of biliary sludge
Presence of gallstone: i.Size ii.Number
Presence of bile duct dilatation
Presence of gall bladder wall thickening

Study Design

Study Type:

Interventional

Actual Enrollment :

3 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Prevention

Official Title:

The Role of Ursodeoxycholic Acid in Treatment of Gallstones in Hemolytic Disorders

Study Start Date :

Dec 1, 2014

Actual Primary Completion Date :

Sep 10, 2017

Actual Study Completion Date :

Sep 10, 2017

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Open Label 32 patients with hemolytic disorders meeting the inclusion and exclusion criteria will be commenced on Ursodeoxycholic acid (UDCA).	Drug: Ursodeoxycholic Acid Patients will be commenced on UDCA 15mg/kg/day (maximum dose 900mg/day) in 2-3 divided doses for 12 months. Patients who are unable to tolerate tablet medication will be started on UDCA syrup at the same dose. Other Names: Ursolit

Arm

Intervention/Treatment

Experimental: Open Label

32 patients with hemolytic disorders meeting the inclusion and exclusion criteria will be commenced on Ursodeoxycholic acid (UDCA).

Drug: Ursodeoxycholic Acid

Patients will be commenced on UDCA 15mg/kg/day (maximum dose 900mg/day) in 2-3 divided doses for 12 months. Patients who are unable to tolerate tablet medication will be started on UDCA syrup at the same dose.

Other Names:

Ursolit

Outcome Measures

Primary Outcome Measures

Lack of sonographic, clinical or biochemical evidence of progression of cholelithiasis or biliary sludge. [12 months]

Secondary Outcome Measures

Improvement in number and severity of episodes of symptomatic gallstones. [12 months]
Rate of surgical intervention for gallstones disease. [12 months]
Sonographic improvement (size and number of gallstones, presence of bile sludge, gallbladder wall thickness). [12 months]
Evaluate tolerability and adverse effects of UDCA therapy in hemolytic disorders. [12 months]
including non-specific abdominal discomfort, rash or nausea.
Improved biochemical evidence of gallstone disease [12m]
reduced gamma-glutamyl-transpeptidase (GGT) and alkaline phosphatase (ALP).

Eligibility Criteria

Criteria

Ages Eligible for Study:

4 Years and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

Diagnosis of a hemolytic disorder including spherocytosis, G6PD deficiency, thalassemia or sickle cell disease.
Patients with cholelithiasis or bile sludge.
Age greater than or equal to 4 years.
Ability to consent to and participate in the study and follow study procedures.

Exclusion Criteria:

Previous splenectomy (complete or partial)
Evidence of hemolytic crisis at the time of research enrollment
Patients with highly symptomatic gallstones who have planned surgical intervention
Known allergy or intolerance to UDCA
Existence of concurrent hepatic disease
Any other laboratory or clinical condition that the investigator considers clinically significant that could impact the outcome of the study or the safety of the patient.