Pyrophosphate Homeostasis and Hepatic Expression of ABCC6.Pyro-TH
Study Details
Study Description
Brief Summary
In animals, normal hepatic expression of ABCC6 (ATP-binding transporter cassette, subfamily C, member 6) determines plasma pyrophosphate (PPi) concentration. PPi prevents the formation of hydroxyapatite crystals on tissues by precipitation of calcium and inorganic phosphate (Pi). It is an endogenous compound whose deficiency causes diffuse vascular calcifications in certain rare monogenic diseases, including the elastic pseudoxanthoma caused by the mutation of ABCC6. PPi is produced by enzymatic transformation of extracellular ATP and, in animals, the liver is the main supplier of ATP and PPi (more than 90%). In humans, liver transplantation offers the possibility of correlating the plasma concentration of PPi ([PPi]pl) with hepatic expression of ABCC6. Liver transplantation is performed in the treatment of chronic liver failure (Child B or C) or, in the absence of liver failure, in the treatment of hepatocellular carcinoma. By measuring[PPi]pl before transplantation and after liver function restoration and by measuring ABCC6 in the diseased liver and healthy liver, it is possible to determine whether liver failure is associated with decreased[PPi]pl and decreased liver expression of ABCC6, which is the objective of our pilot study. Its interest is to establish a physiopathological link between the frequent vascular calcifications in obese patients with hepatic steatosis and the production of PPi.
prupose: Look for a deficit in[PPi]pl in patients before the transplant compared to the phase of restoration of liver function
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Other: cirrhotic patients with chronic liver failure
|
Other: blood sample
Peripheral venous blood is collected before liver transplantation and after restoration of liver function
|
| Other: cirrhotic patients without chronic liver failure
|
Other: blood sample
Peripheral venous blood is collected before liver transplantation and after restoration of liver function
|
Outcome Measures
Primary Outcome Measures
- plasma PPi concentration between before transplantation and liver function restoration in the both arms [24 months]
Eligibility Criteria
Criteria
Inclusion Criteria:
Indication for liver transplantation Age greater than or equal to 18 years Patients affiliated to social security
Exclusion Criteria:
Patient not affiliated to social security Non-consenting patient or unable to understand the protocol and how it works Fulminant Hepatitis
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Untiversity Hospital of Nice | Nice | France | 06000 |
Sponsors and Collaborators
- Centre Hospitalier Universitaire de Nice
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 18-AOI-11