Study of Valproic Acid to Treat HIV Infected Adults

Sponsor

University of North Carolina, Chapel Hill (Other)

Overall Status

Terminated

CT.gov ID

NCT00312546

Collaborator

(none)

Enrollment

Location

Arms

Duration (Months)

0.3

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

A histone deacetylase (HDAC) inhibitor is a class of drug that interferes with the function of HDAC, an enzyme that hides HIV within inactive CD4 cells. These drugs are normally used to treat seizures and other nervous system problems but have been found to work against HIV. The purpose of this study is to investigate the efficacy of valproic acid (VPA), an HDAC inhibitor, in treating HIV infected adults using anti-HIV drugs.

Condition or Disease	Intervention/Treatment	Phase
HIV Infections	Drug: Enfuvirtide Drug: Valproic acid	Phase 1

Detailed Description

VPA is a type of medication normally used to treat seizures and other nervous system problems. It has been found that VPA works against HIV by releasing the virus from resting CD4 cells, allowing other anti-HIV medications to attack it. The purpose of this study is to assess the efficacy of VPA when used in HIV infected participants using highly active antiretroviral therapy (HAART). The expected duration of participation for individually enrolled participants will depend on which study group the participant is placed in but may range from approximately 24 to 144 weeks.

An initial screening visit will occur about 30 to 120 days prior to study entry. A physical exam, medical and medication history assessment, and blood and urine collection will occur at screening. Leukapheresis and genital secretion collection will occur once 30 to 120 days prior to study entry, separate from the initial screening.

In Step 1, all participants will receive VPA while continuing their current HAART. Doses of VPA will vary by participant. Study visits will occur on Days 0 and 3 and Weeks 1, 2, 4, 8, 12, 16, and 24. A physical exam and blood and urine collection will occur at most visits. Leukapheresis and genital secretion collection will occur at study entry and Weeks 12 and 16. After 24 weeks, participants will enter Step 2. Those participants not responding to VPA in Step 1 will enroll in Step 2A. Participants responding to VPA in Step 1 will enroll in Step 2B.

In Step 2A, participants will discontinue VPA and will receive intensified therapy (enfuvirtide) administered for 24 weeks twice daily. Study visits will occur at Weeks 25, 28, 32, 36, 40, and 48. A physical exam and blood and urine collection will occur at all visits. Leukapheresis will occur at Weeks 36 and 40. Participants who do not respond to intensified therapy in Step 2A will enroll in Step 3A. Participants who respond to intensified therapy in Step 2A will enroll in Step 3B.

In Step 2B, participants will continue to receive VPA for up to 96 weeks. Study visits will occur every 8 weeks until Week 120. A physical exam and blood and urine collection will occur at each visit. Leukapheresis will occur once between Week 72 and Week 120.

In Step 3A, VPA will be added to enfuvirtide for 16 weeks. The study will be discontinued for participants who do not respond. Study visits will occur at screening, entry, Day 0, and Weeks 1, 2, 4, 8, 12, 16, and 22. A physical exam and blood and urine collection will occur at all visits. Leukapheresis will occur after screening, at entry, Day 0 and Weeks 12 and 16.

In Step 3B, participants may continue receiving enfuvirtide for up to 96 weeks. Study visits will occur every 8 weeks until Week 144. A physical exam and blood and urine collection will occur at each visit. Leukapheresis will occur at Week 96 or 144.

Participants may choose to enter an observational period at any time before they start Step 2 or Step 3. During the observational period, participants continue to take HAART but not VPA or enfuvirtide. Upon entering an observational period, study staff will contact the participant every 8 weeks for a review of their medical records. Each participant will have a study visit within 8 weeks of beginning a new step. These interim study screenings include a physical exam, medical history, and blood and urine collection. Participants may be asked to have additional leukapheresis performed if they have discontinued study medications for 12 weeks or more.

Each leukapheresis procedure will take place at the University of North Carolina Apheresis Clinic in Chapel Hill, North Carolina. This study will not provide participants' current HAART regimen medications.

NOTE: As of 05/20/08, the observational period and Steps 1, 2, 2A, 2B, and 3B were discontinued.

Study Design

Study Type:

Interventional

Actual Enrollment :

14 participants

Allocation:

Non-Randomized

Intervention Model:

Crossover Assignment

Masking:

None (Open Label)

Primary Purpose:

Treatment

Official Title:

Inhibiting Histone Deacetylase: Toward Eradication of HIV

Study Start Date :

Jun 1, 2006

Actual Primary Completion Date :

Apr 1, 2009

Actual Study Completion Date :

Oct 1, 2009

Arms and Interventions

Arm	Intervention/Treatment
Experimental: 2A Discontinuation of VPA and enfuvirtide administered for 24 weeks. As of 05/20/08 this step was discontinued.	Drug: Enfuvirtide 90 mg subcutaneously twice daily Other Names: T-20
Experimental: 2B Continuation of VPA for up to 96 weeks. As of 05/20/08 this step was discontinued.	Drug: Valproic acid 500 to 750 mg, taken orally twice daily
Experimental: 3A VPA may be added to enfuvirtide for 16 weeks. VPA and enfuvirtide will be continued for up to 96 weeks in responders, and the study will be discontinued in nonresponders.	Drug: Enfuvirtide 90 mg subcutaneously twice daily Other Names: T-20 Drug: Valproic acid 500 to 750 mg, taken orally twice daily
Experimental: 3B Enfuvirtide may be continued for up to 96 weeks. As of 05/20/08 this step was discontinued.	Drug: Enfuvirtide 90 mg subcutaneously twice daily Other Names: T-20

Outcome Measures

Primary Outcome Measures

Frequencies of replication-competent HIV detected in resting CD4 cells [At pre-entry and Week 0 to Weeks 12 and 16]

Secondary Outcome Measures

Change in integrated proviral genomes [Throughout study]
Genital tract proviral DNA and viral load [Throughout study]
Detectable viral load during VPA therapy and if it remains undetectable after VPA is discontinued [Throughout study]
HIV-specific antibody changes and CTL responses [From Week 0 to 16]
Change in replication-competent HIV detected in resting CD4 cells in study participants after intensified HAART, and after extended VPA therapy with or without intensified HAART [Throughout study]
Changes in viral load after intensification of HAART with or without VPA [Throughout study]

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years and Older

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

HIV-1 infected
Adherent to current HAART regimen
Adequate vascular access for leukapheresis
Receiving HAART, defined as at least two nucleoside reverse transcriptase inhibitors plus at least one protease inhibitor or non-nucleoside reverse transcriptase inhibitor, without changes to the regimen within 24 weeks of study entry
Viral load more than 50 copies/ml on two consecutive occasions for more than 6 months, and less than 200 copies/ml on occasion for more than 6 months prior to study entry
CD4 count more than 300 cells/mm3
Willing and able to comply with all study requirements
Willing to use acceptable forms of contraception

Exclusion Criteria:

Currently receiving zidovudine or enfuvirtide
Require certain medications known to interact with valproate (e.g., lamotrigine; barbiturates; carbamazepine; prescription dosages of salicylates, hydantoins, felbamate, and clonazepam)
Any medical, psychiatric, or job-related responsibility that would interfere with the study. More information about this criterion can be found in the protocol.
Contraindications to taking VPA (e.g., pregnancy, bleeding disorders, history of pancreatitis, history of hepatitis)
Receiving interferon, other immunomodulators, or other experimental medications
Abnormal liver enzyme tests
Hepatitis B virus infected
Symptoms of hepatic decompensation
Blood transfusions or hematopoietic growth factors within 90 days prior to study entry
Systemic cytotoxic chemotherapy, investigational agents, or immunomodulators within 90 days prior to study entry
Current drug or alcohol abuse that, in the opinion of the site investigator, would interfere with the study
Serious illness requiring systemic treatment or hospitalization within 90 days prior to study entry
Treatment for a current AIDS-defining opportunistic infection within 90 days prior to screening
Anemic
Involuntarily incarcerated for treatment of either a psychiatric illness or physical illness (e.g., infectious disease)
Pregnancy or breastfeeding