CERA: Human Muscle Gene Expression Profiles in Young and Old Men

Sponsor

Centre Hospitalier Universitaire de Saint Etienne (Other)

Overall Status

Completed

CT.gov ID

NCT00725166

Collaborator

Fondation des Caisses d'Epargne Rhône-Alpes (Other), Institut National de la Recherche Agronomique (Other)

Enrollment

Location

Duration (Months)

8.3

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

To reach the goals of living longer in better medical conditions, many countries reach the same conclusion: new strategies have to be developed to avoid, or at least limit, the effects of age; this requires a better knowledge of the mechanisms of aging. Our project focuses on the loss of muscle mass associated with aging, called sarcopenia. Sarcopenia unavoidably leads to impaired mobility and poor balance, which contributes to loss of functional autonomy and to increased prevalence for severe falls. Skeletal muscle also plays a central role as a reserve for energy and amino acids. Hence, sarcopenia further triggers severe side metabolic effects such as frailty among elderly persons. The precise mechanisms of muscle aging are still mostly unknown, although many theories have been proposed.

The present study aims at better understanding the mechanisms of skeletal muscle loss associated with aging. Using muscle biopsies from young and old subjects, the differential expression profiles of mRNA will be obtained through chips that will evaluate more than 39000 transcripts. On the same samples, proteomic analyses will involve two complementary approaches: (1) bidimensional electrophoresis (2DGE) coupled to mass spectrometry (MALDI-ToF) for dominant proteins; (2) Western-blot (more than 800 antibodies) targeting regulating proteins not detectable using 2DGE. Complementary histological studies (immunohisto-fluorescence, confocal microscopy) will specify the localisation of the major biomarkers in the muscle biopsies.

The results of that research will have applications in the medium term and will lead to nutritional interventions to modulate specific metabolic pathways and improve the quality of life in the elderly.

Condition or Disease	Intervention/Treatment	Phase
Elderly Sarcopenia	Other: Skeletal muscle aging

Study Design

Study Type:

Observational

Actual Enrollment :

41 participants

Observational Model:

Case-Control

Time Perspective:

Prospective

Official Title:

Human Muscle Gene Expression Profiles in Young and Old Men

Study Start Date :

Oct 1, 2008

Actual Primary Completion Date :

Mar 1, 2009

Actual Study Completion Date :

Mar 1, 2009

Arms and Interventions

Arm	Intervention/Treatment
Young Young men 19-25 years old	Other: Skeletal muscle aging W1 - Blood test Maximal voluntary contraction of quadriceps Hydrostatic weighing W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy
Old old men 70-76 years old, who participate in the PROOF study (NCT 00759304)	Other: Skeletal muscle aging W1 - Blood test Maximal voluntary contraction of quadriceps Hydrostatic weighing W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy

Arm

Intervention/Treatment

Young

Young men 19-25 years old

Other: Skeletal muscle aging

W1 - Blood test Maximal voluntary contraction of quadriceps Hydrostatic weighing W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy

Old

old men 70-76 years old, who participate in the PROOF study (NCT 00759304)

Other: Skeletal muscle aging

W1 - Blood test Maximal voluntary contraction of quadriceps Hydrostatic weighing W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy

Outcome Measures

Primary Outcome Measures

To identify the differential expression profiles (proteomics, transcriptomics) in skeletal muscle between young and old men [During biopsie (W3)]

Secondary Outcome Measures

To specify the localisation of the major biomarkers in the muscle biopsies. [During biopsie (W3)]
To compare muscular energy metabolic enzymatic activities between young and old men [during biopsie (W3)]
To compare the number of muscular stem cells between young and old men [during biopsie (W3)]