HAM: Mycoprotein, Resistance Training, and Hypertrophy

Sponsor

University of Exeter (Other)

Overall Status

Active, not recruiting

CT.gov ID

NCT03572127

Collaborator

Quorn (Industry)

Enrollment

Location

Arms

47.5

Anticipated Duration (Months)

0.9

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Protein is vital for the preservation of health and optimal adaptation to training. However, animal proteins come with economic and environmental issues. The study will investigate the effect of non-animal vs animal based habitual protein consumption on muscle protein metabolism and changes in muscle mass and function over a longer period of time.

Condition or Disease	Intervention/Treatment	Phase
Hypertrophy	Other: Diet	N/A

Detailed Description

Protein is vital for the preservation of health and optimal adaptation to training. However, animal proteins come with a number of economic and environmental issues. Accordingly, the investigators need to develop an evidence base for more sustainable dietary proteins to support human nutrition. Mycoprotein is a non-animal protein produced by the continuous fermentation of the fungus Fusarium Venenatum. Previously, in a mechanistic study, it was determined that a single bolus of mycoprotein effectively supports muscle building in both rested and exercised skeletal muscle, to a similar extent as what would be expected of animal proteins. This suggests that similar adaptation of muscle tissue during training could be achieved when protein is obtained from non-animal derived protein sources.

The study will investigate the effect of high non-animal vs animal based habitual protein consumption on muscle protein metabolism and changes in muscle mass and function over a longer period of time. Initially, the study will employ a stable isotope tracer to quantify muscle protein synthesis over a period of three days in healthy male and female volunteers. During this time participants will consume a controlled vegan diet or an animal based diet, with daily unilateral resistance exercise. The second phase of the study will employ a 10 week diet and resistance training intervention, with the majority of a participant's protein either coming from animal or non-animal sources. This will determine the ability of a largely non-animal derived diet to support augmentations in muscle size and strength.

Briefly, participants will undergo 6 muscle biopsies over the course of an 11 week period. Initial biopsies will quantify muscle protein synthesis. Subsequent biopsies will characterise the adaptive response to the effects of nutrition and exercise. MRI scans will be employed to determine changes in muscle volume, and DXA scans will measure changes in body composition.

Study Design

Study Type:

Interventional

Anticipated Enrollment :

42 participants

Allocation:

Randomized

Intervention Model:

Parallel Assignment

Intervention Model Description:

One group will consume a non-animal derived high protein diet, whilst the other group will consume a more traditional animal protein based diet.One group will consume a non-animal derived high protein diet, whilst the other group will consume a more traditional animal protein based diet.

Masking:

None (Open Label)

Primary Purpose:

Basic Science

Official Title:

Does Obtaining the Majority of Dietary Protein From Non-animal Sources Support the Skeletal Muscle Adaptive Response to Structured Resistance Training in Young Men and Women

Actual Study Start Date :

May 16, 2018

Actual Primary Completion Date :

Mar 1, 2020

Anticipated Study Completion Date :

May 1, 2022

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Non-animal derived diet High protein diet derived from non-animal sources.	Other: Diet High protein diet derived from either animal or non-animal sources.
Active Comparator: Animal derived diet High protein diet derived from animal sources.	Other: Diet High protein diet derived from either animal or non-animal sources.

Arm

Intervention/Treatment

Experimental: Non-animal derived diet

High protein diet derived from non-animal sources.

Other: Diet

High protein diet derived from either animal or non-animal sources.

Active Comparator: Animal derived diet

High protein diet derived from animal sources.

Other: Diet

High protein diet derived from either animal or non-animal sources.

Outcome Measures

Primary Outcome Measures

Muscle Protein Synthesis [3 days]
Muscle protein synthesis measured via deuterium oxide

Secondary Outcome Measures

Muscle fiber CSA [10 weeks]
Changes in muscle fiber CSA
Muscle Strength [10 weeks]
Changes in muscle strength
Muscle CSA [10 weeks]
Changes in muscle CSA as measured by MRI

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 40 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

Males and females 18 - 40 years of age.
Body mass index between 18 and 30.
Recreationally active.
Resistance training experienced (have previous performed resistance exercise, and are familiar with the basic movements).

Exclusion Criteria:

Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) (as this may affect normal protein metabolism).
Any diagnosed cardiovascular disease or hypertension (to avoid any complications associated with heavy exercise).
Elevated blood pressure at the time of screening. (An average systolic blood pressure reading of ≥140mmHg over two or more measurements and an average diastolic blood pressure of ≥90mmHg over two or more measurements.)
Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism).
A personal or family history of epilepsy, seizures or schizophrenia.
Allergic to mycoprotein / Quorn, penicillin, or milk.
Pregnancy.