Protein Ingestion Timing on Body Composition and Biochemical Markers in Resistance-trained Males

Sponsor

Marymount University (Other)

Overall Status

Not yet recruiting

CT.gov ID

NCT05544955

Collaborator

Shahid Beheshti University of Medical Sciences (Other)

Enrollment

Location

Arms

3.4

Anticipated Duration (Months)

11.6

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Protein ingestion timing is an important component for muscle accretion. We hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.

Condition or Disease	Intervention/Treatment	Phase
Hypertrophy Physical Performance	Dietary Supplement: isolate whey protein prior to and immediately after resistance training Dietary Supplement: isolate whey protein 3 hours prior to and 3 hours after resistance training	N/A

Study Design

Study Type:

Interventional

Anticipated Enrollment :

40 participants

Allocation:

Randomized

Intervention Model:

Parallel Assignment

Masking:

Triple (Participant, Investigator, Outcomes Assessor)

Primary Purpose:

Basic Science

Official Title:

Comparison of Protein Ingestion Timing on Body Composition, Physical Performance, and Biochemical Markers in Resistance-trained Males

Anticipated Study Start Date :

Sep 17, 2022

Anticipated Primary Completion Date :

Dec 31, 2022

Anticipated Study Completion Date :

Dec 31, 2022

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Protein ingestion immediately prior to and immediately after resistance training This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.	Dietary Supplement: isolate whey protein prior to and immediately after resistance training This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.
Experimental: Protein ingestion 3 hours prior to and 3 hours after resistance training This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.	Dietary Supplement: isolate whey protein 3 hours prior to and 3 hours after resistance training This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Arm

Intervention/Treatment

Experimental: Protein ingestion immediately prior to and immediately after resistance training

This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Dietary Supplement: isolate whey protein prior to and immediately after resistance training

This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Experimental: Protein ingestion 3 hours prior to and 3 hours after resistance training

This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Dietary Supplement: isolate whey protein 3 hours prior to and 3 hours after resistance training

This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Outcome Measures

Primary Outcome Measures

Body Composition [12 weeks]
Total Lean mass, fat mass, and estimated VAT (g) will be assessed using whole-body DXA

Secondary Outcome Measures

Muscular Strength [12 weeks]
Using 1 repetition maximum
Muscular Endurance [12 weeks]
Using leg- and chest press exercises at 75% of the 1-RM
Jump testing [12 weeks]
Using the vertical and broad jump tests
Pull-ups [12 weeks]
Using the pull-up test
Liver enzymes (ALT, AST, and GGT) [12 weeks]
Using Fasting blood samples
Creatinine and Urea Nitrogen [12 weeks]
Urine samples will be collected using standard procedures
Cholesterol Levels [12 weeks]
Using Fasting blood samples

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 35 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

performing resistance training at least three times a week for 1 year prior to the start of the study
not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study
no known medical issues
sleeping for at least seven to eight hours during the 24-hour day

Exclusion Criteria:

non-willingness to continue protein ingestion or exercise protocols
participation in exercise other than the prescribed resistance training program
consumption of dietary supplement (other than the protein intervention) during the study period
Missing more than one resistance training session or protein ingestion throughout the study period

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Marymount University	Arlington	Virginia	United States	22207

Sponsors and Collaborators

Marymount University
Shahid Beheshti University of Medical Sciences

Investigators

None specified.

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

Alexei Wong, Associate Professor, Marymount University

ClinicalTrials.gov Identifier:

NCT05544955

Other Study ID Numbers:

IR.SBMU.MSP.REC.1400. 708

First Posted:

Sep 19, 2022

Last Update Posted:

Sep 19, 2022

Last Verified:

Sep 1, 2022

Studies a U.S. FDA-regulated Drug Product:

Studies a U.S. FDA-regulated Device Product:

Additional relevant MeSH terms:

Hypertrophy

Study Results

No Results Posted as of Sep 19, 2022