Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response

Sponsor

Lund University (Other)

Overall Status

Completed

CT.gov ID

NCT01005342

Collaborator

Vinnova (Other), Oatly AB (Other), Lantmannen Food R&D (Other), Danisco Sugar AB/Fibrex (Other)

Enrollment

Location

Arms

Duration (Months)

17.7

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The aim of this study is to

Measure the effect on gene expression in leukocytes from a meal rich in oat bran
Investigate the postprandial glucose, insulin and triglyceride responses after intake of meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal containing a mixture of these three fibers

Condition or Disease	Intervention/Treatment	Phase
Hypoglycemia Hyperglycemia	Other: Oat bran Other: Spray-dried oat drink Other: Rye bran Other: Sugar beet fiber Other: Mixture of fiber Other: Control	N/A

Detailed Description

Dietary fiber has long been known to give beneficial health effects. Yet, the understanding of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few studies have compared the effects of different fiber sources on postprandial responses and hardly any study the effects of fiber mixtures in the same meal, even though this is more similar to regular eating habits.

Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control). All meals are adjusted to contain the same total amount of available carbohydrates and fat. Blood leukocytes for gene expression profiling were taken before and 2 h after consumption while blood samples for analysis of postprandial glucose, insulin and triglyceride levels were taken every 30 min during 3 h.

NuGO Affymetrix Human Genechip NuGO_Hs1a520180 are used for the microarray analysis and analysis is performed with linear mixed models and enrichment analysis to identify functional gene sets that responded to the specific oat bran effect.

Study Design

Study Type:

Interventional

Actual Enrollment :

18 participants

Allocation:

Randomized

Intervention Model:

Factorial Assignment

Masking:

Single (Participant)

Official Title:

The Influence of Dietary Fibre-rich Meals on Gene Expression in Leukocytes and Postprandial Glucose and Lipid Response in Healthy Subjects

Study Start Date :

May 1, 2007

Actual Primary Completion Date :

Jun 1, 2007

Actual Study Completion Date :

Jun 1, 2007

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Mixture of fiber Single intake of a mixture of spray-dried oat drink, rye bran and sugar beet fiber	Other: Mixture of fiber 38 g spray-dried oat drink, 30 g rye bran and 6 g sugar beet fiber were added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (18 g total fiber).
Experimental: Sugar beet fiber Single intake of sugar beet fiber	Other: Sugar beet fiber 19 g sugar beet fiber was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12 g total fiber). Other Names: Sugar beet Pectin
Experimental: Rye bran Single intake of rye bran	Other: Rye bran 31 g rye bran was added to 250 ml blackcurrant beverage with pulp to give 1.7 g soluble fiber (12 g total fiber). Other Names: Rye Arabinoxylan
Experimental: Oat bran Single intake of oat bran	Other: Oat bran 82 g oat bran was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12.6 g total fiber). Other Names: Oats Beta-glucan
Experimental: Spray-dried oat drink Single intake of spray-dried oat drink	Other: Spray-dried oat drink 62 g spray-dried oat drink was added to 250 ml blackcurrant beverage with pulp to give 2.7 g soluble fiber (3.3 g total fiber). Other Names: Oats Oat milk Beta-glucan
Placebo Comparator: Control Single intake of a meal with no added fiber	Other: Control No fiber was added to control meal (250 g black-currant beverage)