METHYLHOMME: Identification and Characterization of the Methylation Abnormalities on Whole Genome Among Infertile Men
Study Details
Study Description
Brief Summary
This study will analyse the sperm global methylation status of 62 infertile men before assisted reproductive techniques. Some of these patients (20%) present hypomethylation of H19 locus. A global methylation analysis may reveal others imprinting defects.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
An increase of the abnormalities of the imprint was brought back at the child's stemming from assisted reproductive techniques. Now abnormalities of methylation could be implied in defects of spermatogenesis and certain abnormalities of development of the male germ cells could be due to modifications abnormal epigenetics.
The objective of this research is to determine the frequency of arisen the abnormalities of methylation at the level of the locus H19 in the sperm cells of barren men presenting an unexplained oligozoospermia and to determine if these changes are a reflection of abnormalities of the profiles of methylation of the whole genome.
The patients will realize a taking of sperm having signed the consent.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Oligozoospermia infertile patients presenting a reduced sperm count (less than 20 Millions of spermatozoa/ml) |
Other: methylation analyses on spermatozoa from infertile men
microarray analysis(www.EPIGENOMICS.com)
|
Outcome Measures
Primary Outcome Measures
- Bring to light methylation abnormalities of the locus H19 in men's mature sperm cells presenting an unexplained oligozoospermia [1 day]
Secondary Outcome Measures
- Determine if these methylation abnormalities of the locus H19 reflect changes in the profile of global methylation of the spermatic DNA [1 day]
- Estimate the association between these modifications and the nuclear quality of the sperm cell [1 day]
by TUNEL analysis
- Estimate the association between these modifications and the rates of success with In VITRO fertilization [1 day]
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Men from 18 to 45 years old, presenting an idiopathic oligozoospermia lower than 10 million sperm cells / ml and include in a program of medically assisted conception
-
Patients with social security
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Patients having signed the informed consent
Exclusion Criteria:
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Infertility with a neoplastic origin: patients subjected to a treatment potentially sterilizing (chemotherapy or radiotherapy).
-
Infertility with an infectious origin
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Infertility with a traumatic origin
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Infertility bound to a chromosomal abnormality or a microdeletion of Y
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Histories of cryptorchidism, of varicocele
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Department of Biology of reproduction (TENON Hospital) | Paris | Ile de France | France | 75020 |
Sponsors and Collaborators
- Assistance Publique - Hôpitaux de Paris
Investigators
- Principal Investigator: Célia Ravel, MD, TENON Hospital - APHP
Study Documents (Full-Text)
None provided.More Information
Publications
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- AOR 08027