Immune Response to C.Difficile Infection
Study Details
Study Description
Brief Summary
The protocol aims to address the basic mechanisms of Clostridium difficile pathogenesis by identifying how a Th 17 response impacts severity of C. difficile infection and how Type II immunity protects the gut from Clostridium difficile toxin-induced damage. This could lead to new and effective approaches to the treatment or prevention of Clostridium difficile colitis that act downstream of fecal microbiota transplants (FMT) or next generation probiotics. Successful fecal microbial transplantation will restore protective immunity to recurrent C.difficile infection.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Detailed Description
The study includes one cohort of hospitalized patients with acute CDI who may require diagnostic colonoscopy, a second cohort of outpatients with recurrent CDI scheduled for FMT and a third cohort of inpatients with past history of CDI without recurrence.
Blood samples and discarded stool samples for research will be obtained from adult hospitalized patients. Biopsies and brushing samples for research will be obtained from patients requiring diagnostic colonoscopies for clinical care. Follow-up will include phone contact at 60-90 days to determine relapse or mortality in acute CDI patients.
Blood and colonic biopsies and brushing samples will be obtained from patients undergoing FMT for recurrent CDI and again after 60 days from convalescent patients.
Blood and biopsies taken for research purposes at each colonoscopy will be analyzed for:
cytokines and chemokines, gene expression analysis, immunohistochemistry and high dimensional flow-cytometry.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Acute CDI cohort Hospitalized patients diagnosed with Acute CDI |
|
| FMT cohort Patients undergoing FMT for recurrent CDI |
|
| Past CDI Control Cohort Hospitalized patients with past CDI diagnosis without recurrence |
Outcome Measures
Primary Outcome Measures
- Adaptive immune response [0-60 days post enrollment]
Assessment of adaptive immunity including Th1, Th2 and TH17 immune response
Secondary Outcome Measures
- Changes in gut health [0-60 days post enrollment]
Association of biomarkers in stool and biopsy specimens with CDI outcome
- Gene expression of immune cells in colon [0-60 days post enrollment]
Profiling colonic gene expression and mucosal immune pathways in CDI
- Microbiome [0-60 days post enrollment]
Tissue 16s rDNA
- Immunohistochemistry [0-60 days post enrollment]
Changes in mucosal immunity following FMT
- Antibody response to C. difficile infection [0-60 days post enrollment]
IgG and IgA to C. difficile antigens in plasma and stool
- High dimensional flow-cytometry [0-60 days post enrollment]
Profiling of immune cells in blood and biopsy
Eligibility Criteria
Criteria
Inclusion Criteria:
-Acute CDI cohort
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Acute CDI diagnosis including PCR positive fecal samples
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Optional diagnostic colonoscopy for clinical care
FMT cohort
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At least one relapse or recurrence of C. difficile infection
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Eligible for fecal microbiota transplant (FMT)
Past CDI cohort
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Past CDI diagnosis and current PCR negative fecal samples
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Optional diagnostic colonoscopy for clinical care
Exclusion Criteria:
Acute CDI cohort:
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Unwilling to have research biopsies and brushings at time of diagnostic colonoscopy; Unwilling to provide blood and stool samples (discarded stool from UVA lab) for research
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Unwilling to participate in follow-up phone call at 60-90 days
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Concurrent participation in another clinical trial. This exclusion does not apply to participation in IRB-HSR #200046 and non-interventional research studies. Concurrent participation in non-interventional research studies is allowed.
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Clinical contraindication to colonoscopy or conscious sedation
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Pregnancy
-
Inability to give informed consent unless a legally authorized representative (LAR) is available
-
Incarceration
-
HIV infection
FMT cohort:
-
Unwilling to have research biopsies and brushings and stool samples at time of colonoscopy with FMT for clinical care and research sigmoidoscopy at Day 60
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Unwilling to provide blood samples for research
-
Concurrent participation in another clinical trial This exclusion does not apply to participation in non-interventional research studies. Concurrent participation in non-interventional research studies is allowed.
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Clinical contraindication to sigmoidoscopy or conscious sedation
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Pregnancy
-
Inability to give informed consent
-
Incarceration
-
HIV infection
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Neutropenia (<1000 PMNs/µl blood)
Past CDI Control cohort:
-
Unwilling to have research biopsies and brushings at time of diagnostic colonoscopy; Unwilling to provide blood and stool samples (discarded stool from UVA lab) for research
-
Concurrent participation in another clinical trial. This exclusion does not apply to participation in IRB-HSR #200046 and non-interventional research studies. Concurrent participation in non-interventional research studies is allowed.
-
Clinical contraindication to colonoscopy or conscious sedation
-
Pregnancy
-
Inability to give informed consent unless a legally authorized representative (LAR) is available
-
Incarceration
-
HIV infection
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | University of Virginia Health System | Charlottesville | Virginia | United States | 22903 |
Sponsors and Collaborators
- University of Virginia
- National Institute of Allergy and Infectious Diseases (NIAID)
Investigators
- Principal Investigator: William A. Petri, MD,PhD, University of Virginia
Study Documents (Full-Text)
More Information
Publications
None provided.- 18782
- R01AI124214-01