FLUORIB: Impact of 5-Fluorouracil (5-FU) on Cancer Cells From Liver Metastases of Colon Cancer
Study Details
Study Description
Brief Summary
Analyze the dynamics of incorporation of 5-fluorouracil into cancer cell ribosomes from liver metastases of patient with metastatic colon cancer.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
This is a monocentric, prospective, non-interventional cohort study in adult patients with metastatic colon cancer with hepatics metastasis.
Tumour samples collected for this study include:
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A fragment of the previously operated primary tumor: FFIP block and/or archived frozen tumor if available.
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A fragment of the liver metastasis(s) performed as part of standard metastasis surgery, this fragment will include a tumor area and a healthy area within the margin of excision. These samples collected in a fresh state will be analyzed immediately.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Metastatic colon cancer Patient with metastatic colon cancer operated on and followed up at the Centre Leon Berard for liver metastasis. |
Procedure: Scheduled and planned surgery
Collection of tumour samples from samples collected as part of standard patient management. Diagnostic pathological analysis will be a priority. These samples will be taken by experienced staff during the hospital visit scheduled as part of standard care.
|
Outcome Measures
Primary Outcome Measures
- 5-FU in ribosome [Up to 24 months]
Rate of incorporation of 5-fluorouracil into ribosomes of tumor tissues and adjacent healthy tissues before surgery and after surgery.
Secondary Outcome Measures
- Methylation profile of ribosomal RNAs [Up to 24 months]
Analyze on a large scale the alterations of the 2'-O-methylation profile of ribosomal RNAs before surgery and after surgery
- Transcriptomic and proteomic profile [Up to 24 months]
Define a transcriptomic and proteomic profile of the collected samples before surgery and after surgery.
- Specific molecular signature [Up to 24 months]
Combination of the number of 5-fluorouracil molecules incorporated per rRNA molecule, the methylation profile and the transcriptomic profile before surgery and after surgery.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Patient with metastatic colon cancer operated on and followed up at the Centre Leon Berard for liver metastasis.
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Patient affiliated to social security
Exclusion Criteria:
- Other pathologies that may interfere with biological evaluation, in particular known hepatic cirrhosis, whatever its origin, other liver overload diseases (hemochromatosis, Wilson's disease), but excluding steatosis (which can be chemo-induced).
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Centre Léon Bérard | Lyon | France | 69008 |
Sponsors and Collaborators
- Centre Leon Berard
Investigators
- Principal Investigator: Philippe CASSIER, MD, Centre Leon Berard
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- ET 19-127 FLUORIB