IVOIRE: Impact of Intestinal Virome on Pediatric Inflammatory Bowel Disease
Study Details
Study Description
Brief Summary
Over the last few years, dysbiosis has emerged as a possible trigger of gut inflammation in inflammatory bowel disease (IBD) and a promising therapeutic target. The complex diversity of microbiota was initially highlighted by the powerful new tools in genetics, including next-generation sequencing (NGS). NGS permitted to decipher the composition of bacterial intestinal communities, but also that of the gut virome. Since then, the evidence of a dynamic instability of the enteric virome in IBD has grown considerably. IBD patients present an expansion of bacteriophages (Caudovirales) associated with decreased bacterial diversity. Moreover, gut virome richness seems to differ between Crohn's disease (CD) and ulcerative colitis (UC) patients. These insights open the gate of new diagnostic, predictive, and therapeutic approaches. However, little is known about pediatric IBD gut virome in terms of variability and evolution under the influence of different treatments (exclusive enteral nutrition, immunosuppressive therapy and biologics). The aim of this study is to evaluate the gut family viral diversity and relative abundance of eukaryotes and prokaryotes in paediatric IBD patients
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Study Design
Outcome Measures
Primary Outcome Measures
- Evaluation over time of change of the gut virome [at the inclusion, 6 months and one year]
Abundance measure: number of sequences generated for a given family or species. Viral isolation, extraction, and amplification of viral nucleic acids from patient's stools. Next generation sequencing Statistical analysis
- Evaluation over time of change of the gut virome [at the inclusion, 6 months and one year]
Measure of relative abundance: abundance of a given family or species relative to other species or families in the sample. eukaryote versus prokaryote virus Viral isolation, extraction, and amplification of viral nucleic acids from patient's stools. Next generation sequencing Statistical analysis
- Evaluation over time of change of the gut virome [at the inclusion, 6 months and one year]
Measure of alpha diversity by the Shannon index which takes into account the number of species present, but also the distribution of these species. Viral isolation, extraction, and amplification of viral nucleic acids from patient's stools. Next generation sequencing Statistical analysis
Eligibility Criteria
Criteria
Inclusion Criteria:
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Age: 6-17 years
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Follow-up in pediatric gastroenterology for inflammatory bowel disease :
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Crohn's disease
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Hemorrhagic rectocolitis
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Introduction of anti-TNFa treatment in the Pediatric Gastroenterology Day Hospital of the "Hôpital Femme Mère Enfant" service in Lyon
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Collection of the non-opposition of at least one of the holders of the parental authority present and the child in the medical file
Exclusion Criteria:
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Refusal to participate in the study
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Antibiotherapy in the 4 weeks preceding the sampling
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Patient with ileostomy or colostomy.
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Patient who has undergone extensive bowel resection.
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History of intestinal surgery (except appendectomy)
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Patient subject to a legal protection measure
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Service de gastroentérologie nutrition, hépatologie pédiatrique - Hôpital Femme Mère Enfant groupement hospitalier Est - HCL | Bron | France | 69677 |
Sponsors and Collaborators
- Hospices Civils de Lyon
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 69HCL18_0794