EA: Rate-limiting Amino Acids in Endurance-trained Athlete

Sponsor

University of Toronto (Other)

Overall Status

Completed

CT.gov ID

NCT02628249

Collaborator

(none)

Enrollment

Arms

Actual Duration (Months)

Study Details

Study Description

Brief Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

The biological value of protein depends on the amino acid composition. As liebig's law of the minimum, the protein synthesis is limited due to the lack of single rate limiting amino acid. In this case, the first rate limiting amino acid determines protein intakes to maximize the protein synthesis. Adding a small amount of rate-limiting amino acid to the diet improves the biological value of its protein diet.

The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. however, the IAAO method has never been utilized for determining the first rate limiting amino acid.

Therefore, the purpose of this study is to address of IAAO method to be applied for determining the rate-limiting amino acid in endurance athlete.

Condition or Disease	Intervention/Treatment	Phase
Increased Metabolic Requirement Healthy	Dietary Supplement: Base protein Dietary Supplement: Sufficient protein Dietary Supplement: Branched chain amino acids Dietary Supplement: Essential amino acids Dietary Supplement: Non essential amino acids	N/A

Study Design

Study Type:

Interventional

Actual Enrollment :

5 participants

Allocation:

Randomized

Intervention Model:

Crossover Assignment

Masking:

Single (Participant)

Primary Purpose:

Basic Science

Official Title:

Application of Indicator Amino Acid Oxidation Method to Investigate the Rate-limiting Amino Acids in Endurance Trained Athlete

Actual Study Start Date :

Sep 1, 2015

Actual Primary Completion Date :

Jun 1, 2017

Actual Study Completion Date :

Dec 1, 2017

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Base protein 0.8 g/kg/d of protein provided as crystalline amino acid made after egg protein.	Dietary Supplement: Base protein 0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein
Experimental: Sufficient protein 1.75 g/kg/d protein provided as crystalline amino acid made after egg protein.	Dietary Supplement: Sufficient protein 1.75 g/kg/d protein provided as crystalline amino acid modeled after egg protein.
Experimental: Base + BCAA Base protein intake + Branched chain amino acids	Dietary Supplement: Base protein 0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein Dietary Supplement: Branched chain amino acids Branched chain amino acids modeled after egg protein.
Experimental: Base + EAA Base protein intake + essential amino acids.	Dietary Supplement: Base protein 0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein Dietary Supplement: Essential amino acids Essential amino acids modeled after egg protein.
Experimental: Base + NEAA Base protein intake + non essential amino acids	Dietary Supplement: Base protein 0.8 g/kg/d of protein provided as crystalline amino acid modeled after egg protein Dietary Supplement: Non essential amino acids Non essential amino acids modeled after egg protein.

Outcome Measures

Primary Outcome Measures

13CO2 (carbon dioxide) excretion rate (μmol/kg/h) [At 8 hours after the end of exercise]
8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.

Secondary Outcome Measures

[13C]phenylalanine oxidation rate (μmol/kg/h) [at 8 hours after the end of exercise]
8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined dividing 13CO2 excretion rate by urinary enrichment of 13C-phenylalanine in breath measured by Mass spectrometry, and CO2 production rate measured by metabolic cart.

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 35 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Gender: Male

Age limit: Minimum age 18 years - Maximum age 35 years

Accepts Healthy Volunteers : yes

Inclusion Criteria:

Endurance-trained participants who regularly more than 40 km/week
Ability to perform the exercise stimulus (20 km run) on metabolic trial.

Exclusion Criteria:

Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
Regular tobacco use
Illicit drug use

Contacts and Locations

Locations

No locations specified.

Sponsors and Collaborators

University of Toronto

Investigators

None specified.

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

Daniel Moore, Assistant professaor, University of Toronto

ClinicalTrials.gov Identifier:

NCT02628249

Other Study ID Numbers:

IAAO-EA

First Posted:

Dec 11, 2015

Last Update Posted:

Apr 1, 2019

Last Verified:

Mar 1, 2019

Study Results

No Results Posted as of Apr 1, 2019