Effect of Oral Zinc Sulfate Supplementation on Enzymes of Urate Pathway

Sponsor

Babylon University (Other)

Overall Status

Completed

CT.gov ID

NCT03361618

Collaborator

Ministry of Health, Iraq (Other)

120

Enrollment

Location

Arms

Actual Duration (Months)

10.9

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Although many reports have investigated the relationship between subfertility and uric acid levels in semen, to the best of author knowledge, there is no study have been published to yet focus on the effects of asthenospermia treatments, such as given an oral zinc supplementation, on the activity of urate-related enzymes, which are important in fertility of human.

Condition or Disease	Intervention/Treatment	Phase
Infertility Unexplained	Dietary Supplement: Zinc Sulfate	N/A

Detailed Description

The present study was designed to investigate the effect of zinc supplementation on the qualitative and quantitative characteristics of semen, along with uric acid levels and urate-related enzymes in the seminal plasma and spermatozoa of asthenospermic males.

Study Design

Study Type:

Interventional

Actual Enrollment :

120 participants

Allocation:

Non-Randomized

Intervention Model:

Parallel Assignment

Intervention Model Description:

Semen volume after treatment.Semen volume after treatment.

Masking:

None (Open Label)

Primary Purpose:

Treatment

Official Title:

Effect of Oral Zinc Sulfate Supplementation on Enzymes of Urate Pathway in Spermatozoa and Seminal Plasma of Iraqi Asthenospermic Patients

Actual Study Start Date :

Jul 1, 2011

Actual Primary Completion Date :

Jun 1, 2012

Actual Study Completion Date :

Jun 1, 2012

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Zinc sulfate 60 subfertile (age 32.5±3.23 year) men with asthenozoospermia was treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg).	Dietary Supplement: Zinc Sulfate The subfertile group treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg).
No Intervention: Healthy control 60 fertile (age 31.6±3.3 year) men, no treatment.

Arm

Intervention/Treatment

Experimental: Zinc sulfate

60 subfertile (age 32.5±3.23 year) men with asthenozoospermia was treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg).

Dietary Supplement: Zinc Sulfate

The subfertile group treated with zinc sulfate, every participant took two capsules of zinc sulfate per day for three months (each one 220 mg).

No Intervention: Healthy control

60 fertile (age 31.6±3.3 year) men, no treatment.

Outcome Measures

Primary Outcome Measures

Measurement the uric acid level in spermatozoa and seminal plasma by using spectrophotometer. [up to three months.]
Clinical parameter

Secondary Outcome Measures

Measurement the adenosine deaminase activity in spermatozoa and seminal plasma by using spectrophotometer. [up to three months.]
Enzyme
Measurement the xanthine oxidase activity in spermatozoa and seminal plasma by using spectrophotometer. [up to three months.]
Enzyme
Measurement the 5'-nucleotidase activity in spermatozoa and seminal plasmaby using the spectrophotometer. [up to three months.]
Enzyme

Eligibility Criteria

Criteria

Ages Eligible for Study:

26 Years to 36 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

The presence of asthenozoospermia in the semen sample.
Must be unable to reproduce.

Exclusion Criteria:

the absence of endocrinopathy, varicocele, and female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.