Comparison of the Effect of Microchip and Density Gradient Methods in Intrauterine Insemination Cycles
Study Details
Study Description
Brief Summary
Microfluidic chips are one of the methods of sperm separation to eliminate DNA fragmentation in sperm. It is thought that the separation of sperm by centrifugation in the classical gradient density (Percoll) method used in sperm separation in IVF and IUI cycles leads to the increase of reactive oxygen radicals in sperm and this leads to sperm DNA fragmentation. Studies comparing Percoll and microfluidic chip method in terms of sperm, embryo quality and pregnancy rates are limited. In this context, it is aimed to investigate the effect of Percoll or Microfluidic Chip Technology on the quality of sperms and embryos obtained with these sperms and their pregnancy rates prospectively.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
The increase in infertility rate due to environmental and physiological conditions leads to an increase in the use of assisted reproductive techniques. Isolation of living and morphologically normal live sperm is an integrated procedure in commonly used IVF / ICSI(intracytoplasmic sperm injection) / IUI(intrauterine insemination) procedures. Although current IUI procedures result in a successful pregnancy of around 10-15%, the process can be greatly compromised if the selected sperm is abnormal. Microfluidic chips are one of the recommended sperm separation methods to eliminate DNA fragmentation in sperm. It is thought that the separation of the sperm by centrifugation in the classical gradient density (Percoll) method which is used in the separation of sperm in the IUI cycles, causes the increase of reactive oxygen radicals in sperm and this leads to sperm DNA fragmentation. Studies comparing Percoll and microfluidic chip method in terms of sperm, embryo quality and pregnancy rates are limited. In this context, it is aimed to investigate the effect of Percoll or Microfluidic Chip Technology on the quality of sperms and embryos obtained with these sperms and their pregnancy rates prospectively.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: MicroFluidic Sperm Sorting Chips Sperm Sorting microfluidic chips will be used when preparing sperm of male partner and IUI will be made with separated sperm |
Other: MicroFluidic Sperm Sorting Chips
sperm selection of IUI treatment
|
| Active Comparator: gradient-density centrifugation gradient-density centrifugation technique will be used when preparing sperm of male partner and IUI will be made with separated sperm |
Other: Gradient-Density Centrifugation
sperm selection of IUI treatment
|
Outcome Measures
Primary Outcome Measures
- Compare microfluid chip and density-gradient methods in terms of IUI success [average of 6 months]
measure the clinical pregnancy rate by using serum beta-HCG (human chorionic gonadotropin) level
- Compare microfluid chip and density-gradient methods in terms of embryo quality [average of 6 months]
Embryo morphology will be assessed on day 3 using the standard criteria of the number of blastomeres and extent of fragmentation and blastomere asymmetry. Top quality embryos on day 3 will be designated as embryos with 7-8 cells, ≤10% fragmentation, and symmetric blastomeres. Using these criteria, the rate of top quality embryos will be analyzed.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Apply to the infertility clinic due to unexplained infertility and mild male factor (total motile sperm count >5 million)
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Volunteer
Exclusion Criteria:
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Inadequate follicle development with medication
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Embryo does not have the appropriate quality for transfer
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Tubal pathology
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Total motile sperm count < 5 million
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Refuse to participate in research
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Bezmialem University | Istanbul | Turkey | 34746 |
Sponsors and Collaborators
- Bezmialem Vakif University
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
- Gode F, Bodur T, Gunturkun F, Gurbuz AS, Tamer B, Pala I, Isik AZ. Comparison of microfluid sperm sorting chip and density gradient methods for use in intrauterine insemination cycles. Fertil Steril. 2019 Nov;112(5):842-848.e1. doi: 10.1016/j.fertnstert.2019.06.037. Epub 2019 Sep 19.
- Yetkinel S, Kilicdag EB, Aytac PC, Haydardedeoglu B, Simsek E, Cok T. Effects of the microfluidic chip technique in sperm selection for intracytoplasmic sperm injection for unexplained infertility: a prospective, randomized controlled trial. J Assist Reprod Genet. 2019 Mar;36(3):403-409. doi: 10.1007/s10815-018-1375-2. Epub 2018 Dec 12.
- Microchip IUI study