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AEX: Impact of Acute Exercise Intensity and Pattern on Cytokine Function

Sponsor
University of British Columbia (Other)
Overall Status
Recruiting
CT.gov ID
NCT05574413
Collaborator
(none)
20
Enrollment
1
Location
4
Arms
11.5
Anticipated Duration (Months)
1.7
Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The immune system helps prevent illness, fights off infections, and repairs damaged tissues following an injury. However, when immune cells remain active for prolonged periods of time - a state known as "chronic inflammation" - they can contribute to the development and progression of chronic diseases like heart disease and diabetes. Exercise can reduce the risk of developing many of these diseases and at least part of the health benefits of exercise are due to the ability of exercise to reduce "chronic inflammation". The inflammation-lowering effects of exercise are typically captured by measuring hormone-like molecules released from immune cells called "cytokines" in the blood. In addition to changes in circulating cytokine levels, exercise may also alter how immune cells respond to these cytokines. How exercise intensity (i.e., how hard you are working during exercise) and pattern (i.e., exercising as a long continuous bout or in short intervals) impact the ability of immune cells to respond to cytokines is not well understood. A better understanding of how exercise intensity and pattern of exercise for reducing chronic inflammation may help determine the best types of exercises for improving health and preventing chronic diseases.

Condition or Disease Intervention/Treatment Phase
  • Other: Moderate intensity continuous exercise
  • Other: High intensity continuous exercise
  • Other: High intensity interval exercise
  • Other: Resting (no exercise) control
 N/A

Study Design

Study Type:
Interventional
Anticipated Enrollment :
20 participants
Allocation:
Randomized
Intervention Model:
Crossover Assignment
Intervention Model Description:
Repeated measures randomized counterbalanced crossover designRepeated measures randomized counterbalanced crossover design
Masking:
None (Open Label)
Primary Purpose:
Basic Science
Official Title:
Impact of Acute Exercise Intensity and Pattern on Cytokine Function
Actual Study Start Date :
Sep 15, 2022
Anticipated Primary Completion Date :
Apr 1, 2023
Anticipated Study Completion Date :
Sep 1, 2023

Arms and Interventions

Arm Intervention/Treatment
Placebo Comparator: Resting (no exercise) control

Resting (no exercise) control condition

Other: Resting (no exercise) control
Participants will remain in a rested state (i.e., no exercise) for the entire session. Blood samples will be obtained at the same time-points as the exercise sessions.
Other Names:
  • CTL

    		•
    Experimental: Moderate intensity continuous exercise (MICE)

    Experimental session involving an acute bout of moderate intensity continuous exercise (MICE; continous cycling expending 350 kcal at 70% of lactate threshold)

    Other: Moderate intensity continuous exercise
    Participants will perform an acute bout of continuous cycling at 70% of the power output at lactate threshold until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
    Other Names:
  • MICE

    		•
    Experimental: High intensity continuous exercise (HICE)

    Experimental session involving an acute bout of high intensity continuous exercise (HICE; continuous cycling expending 350 kcal at 10% of the difference between lactate threshold and VO2peak)

    Other: High intensity continuous exercise
    Participants will perform an acute bout of continuous cycling at 10% of the difference between lactate threshold and VO2peak until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
    Other Names:
  • HICE

    		•
    Experimental: High intensity interval exercise (HIIT)

    Experimental session involving an acute bout of high intensity interval exercise (HIIT; cycling intervals expending 350 kcal at 10% of the difference between lactate threshold and VO2peak)

    Other: High intensity interval exercise
    Participants will perform an acute bout of interval cycling at at 10% of the difference between lactate threshold and VO2peak until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
    Other Names:
  • HIIE

    		•

    Outcome Measures

    Primary Outcome Measures

    1. IL-10 mediated STAT3 phosphorylation [Change from pre-exercise to immediately and 90-min post-exercise]

      Ex vivo leukocyte STAT3 phosphorylation in response to IL-10 treatment

    Secondary Outcome Measures

    1. IL-10 mediated TNF-alpha inhibition [Change from pre-exercise to immediately and 90-min post-exercise]

      Ex vivo inhibition of TNF-alpha production in response to IL-10 treatment

    2. IL-6 mediated STAT3 phosphorylation [Change from pre-exercise to immediately and 90-min post-exercise]

      Ex vivo leukocyte STAT3 phosphorylation in response to IL-6 treatment

    3. IL-6 mediated TNF-alpha inhibition [Change from pre-exercise to immediately and 90-min post-exercise]

      Ex vivo inhibition of TNF-alpha production in response to IL-6 treatment

    4. Plasma IL-10 [Change from pre-exercise to immediately, 30-, and 90-min post-exercise]

      Concentration of IL-10 in plasma samples

    5. Plasma IL-6 [Change from pre-exercise to immediately, 30-, and 90-min post-exercise]

      Concentration of IL-6 in plasma samples

    6. Plasma TNF-alpha [Change from pre-exercise to immediately, 30-, and 90-min post-exercise]

      Concentration of TNF-alpha in plasma samples

    7. Hematology panel [Change from pre-exercise to immediately, 30-, and 90-min post-exercise]

      Complete blood count

    8. Extracellular vesicles [Change from pre-exercise to immediately, 30-, and 90-min post-exercise]

      Concentration of extracellular vesicles in plasma

    Eligibility Criteria

    Criteria

    Ages Eligible for Study:
    18 Years to 35 Years
    Sexes Eligible for Study:
    All
    Accepts Healthy Volunteers:
    Yes
    Inclusion Criteria:

    • 18-35 years of age

    • Body mass index between 18.5-30 kg/m^2

    • Free of cardiometabolic and autoimmune/inflammatory disease

    Exclusion Criteria:

    • Competitive endurance athlete

    • Cigarette smoker

    • Currently taking immunomodulatory/anti-inflammatory medications

    • Currently pregnant

    Contacts and Locations

    Locations

    Site City State Country Postal Code
    1 UBC Okanagan Kelowna British Columbia Canada V1V1V7

    Sponsors and Collaborators

    • University of British Columbia

    Investigators

    • Principal Investigator: Jonathan P Little, PhD, UBC Okanagan

    Study Documents (Full-Text)

    None provided.

    More Information

    Publications

    None provided.
    Responsible Party:
    Jonathan Little, Professor, University of British Columbia
    ClinicalTrials.gov Identifier:
    NCT05574413
    Other Study ID Numbers:
    • AEX2022
    First Posted:
    Oct 10, 2022
    Last Update Posted:
    Oct 10, 2022
    Last Verified:
    Oct 1, 2022
    Studies a U.S. FDA-regulated Drug Product:
    No
    Studies a U.S. FDA-regulated Device Product:
    No
    Keywords provided by Jonathan Little, Professor, University of British Columbia
    Exercise
    Cytokines
    Leukocytes
    Interleukin-10
    Interleukin-6
    Additional relevant MeSH terms:
    Inflammation

    Study Results

    No Results Posted as of Oct 10, 2022