Interaction Between Immune Cells and Bacteria Associated With Periodontitis

Sponsor

University of Copenhagen (Other)

Overall Status

Completed

CT.gov ID

NCT03225950

Collaborator

Copenhagen University Hospital, Denmark (Other)

Enrollment

Locations

37.7

Actual Duration (Months)

Patients Per Site

1.2

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

This study evaluates the interaction between host immune cells and bacteria associated with periodontitis. It comprises biological material from donors with and without periodontal disease. Specifically, we collect a spit and blood sample to conduct in vitro stimulations and measurements of selected parameters related to periodontitis to clarify obscure areas in the immunologic pathogenesis of this disease.

Condition or Disease	Intervention/Treatment	Phase
Periodontal Diseases Periodontitis Aggressive Periodontitis Immunologic Disease Microbial Disease Periodontal Pocket Inflammation Inflammation Gum Dysbiosis Rheumatoid Arthritis Generalized Aggressive Periodontitis Generalized Chronic Periodontitis Chronic Periodontitis	Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers Genetic: Analysis of selected single nucleotide polymorphisms (SNPs) Diagnostic Test: periodontitis-associated bacteria presence

Detailed Description

Periodontitis is a prevalent, multifactorial inflammatory disease characterized by the interaction between microorganisms organized in biofilms on tooth surfaces and host immune cells, leading to an inflammatory destruction of the tooth-supporting tissues and - if left untreated - eventually tooth loss. Periodontitis affects up to 50% of the population in the United States of America, and is classified in an aggressive and a chronic form depending on genetic factors, age of onset, speed and severity of attachment loss.

The onset of periodontitis is caused by an immunologic imbalance between host immune cells and residing microorganisms in subgingival pockets. The host immune cells are capable of enhancing both a protective and a destructive inflammatory response towards the microorganisms through the release of inflammatory mediators e.i. proinflammatory and antiinflammatory cytokines.

The role of antibodies in periodontitis is also unclear. Some studies show an excessive antibody level against bacteria associated with periodontitis e.g. Porphyromonas gingivalis (P.g.).

In general, this study contributes to a profound understanding of the host immune cells role in the onset and pathogenesis of periodontitis by comparing healthy versus diseased donors immunologic responses toward pathogene and apathogene microorganisms and their genetic background.

Study Design

Study Type:

Observational

Actual Enrollment :

90 participants

Observational Model:

Case-Control

Time Perspective:

Cross-Sectional

Official Title:

Interaction Between Immune Cells and Bacteria Associated With Periodontitis

Actual Study Start Date :

Feb 1, 2017

Actual Primary Completion Date :

Oct 12, 2018

Actual Study Completion Date :

Mar 23, 2020

Arms and Interventions

Arm	Intervention/Treatment
Chronic periodontitis donors Donors are medically healthy. Slow to moderate attachment loss and bone destruction. Good correlation between etiological factors and serverity of attachment loss.	Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria Peripheral mononuclear blood cells are stimulated with periodontitis-associated- and control bacteria to measure the amount of positive cytokine-producing cells. Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers Anitbody titers will be measured in saliva and serum samples. Genetic: Analysis of selected single nucleotide polymorphisms (SNPs) DNA obtained from saliva samples will be used to determine the genotype of the participants for selected SNPs. Diagnostic Test: periodontitis-associated bacteria presence Determination of the presence of periodontitis-associated bacteria e.i. Porphyromonas gingivalis in saliva and blood samples.
Aggressive periodontitis donors Donors are medically healthy. Rapid attachment loss and bone destruction. Familial aggregation. No correlation between etiological factors and serverity of attachment loss.	Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria Peripheral mononuclear blood cells are stimulated with periodontitis-associated- and control bacteria to measure the amount of positive cytokine-producing cells. Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers Anitbody titers will be measured in saliva and serum samples. Genetic: Analysis of selected single nucleotide polymorphisms (SNPs) DNA obtained from saliva samples will be used to determine the genotype of the participants for selected SNPs. Diagnostic Test: periodontitis-associated bacteria presence Determination of the presence of periodontitis-associated bacteria e.i. Porphyromonas gingivalis in saliva and blood samples.
Control donors Donors are medically healthy. No sign of inflammatory conditions.	Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria Peripheral mononuclear blood cells are stimulated with periodontitis-associated- and control bacteria to measure the amount of positive cytokine-producing cells. Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers Anitbody titers will be measured in saliva and serum samples. Genetic: Analysis of selected single nucleotide polymorphisms (SNPs) DNA obtained from saliva samples will be used to determine the genotype of the participants for selected SNPs. Diagnostic Test: periodontitis-associated bacteria presence Determination of the presence of periodontitis-associated bacteria e.i. Porphyromonas gingivalis in saliva and blood samples.

Arm

Intervention/Treatment

Chronic periodontitis donors

Donors are medically healthy. Slow to moderate attachment loss and bone destruction. Good correlation between etiological factors and serverity of attachment loss.

Other: In vitro stimulation of blood with periodontitis-associated- and control bacteria

Peripheral mononuclear blood cells are stimulated with periodontitis-associated- and control bacteria to measure the amount of positive cytokine-producing cells.

Diagnostic Test: Anti-CCP- and anti-P.g.-antibodies titers

Anitbody titers will be measured in saliva and serum samples.

Genetic: Analysis of selected single nucleotide polymorphisms (SNPs)

DNA obtained from saliva samples will be used to determine the genotype of the participants for selected SNPs.

Diagnostic Test: periodontitis-associated bacteria presence

Determination of the presence of periodontitis-associated bacteria e.i. Porphyromonas gingivalis in saliva and blood samples.

Aggressive periodontitis donors

Donors are medically healthy. Rapid attachment loss and bone destruction. Familial aggregation. No correlation between etiological factors and serverity of attachment loss.