The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling
Study Details
Study Description
Brief Summary
The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a [2H5]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Active Comparator: Collagen and Vitamin C
|
Dietary Supplement: Collagen and Vitamin C
Collagen-modelled crystalline amino acids (30g total, 8g glycine) and vitamin C (500 mg).
Other Names:
|
| Placebo Comparator: Carbohydrate Placebo
|
Dietary Supplement: Carbohydrate Placebo
Iso-caloric Placebo (30g maltodextrin).
|
Outcome Measures
Primary Outcome Measures
- Muscle Collagen Enrichment [9 hours]
Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into mature collagen protein isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS.
Secondary Outcome Measures
- Localization of Collagen Remodelling [9 hours]
Immunofluorescence determined localization of collagen breakdown and synthesis using collagen hybridizing peptide and procollagen antibodies, respectively, on human skeletal muscle cross-sections.
- Urinary Growth Hormone [8 hours]
Urine will be collected overnight to measure growth hormone secretion using chemiluminescence.
- Myofibrillar Protein Enrichment [9 hours]
Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into myofibrillar proteins isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS.
Other Outcome Measures
- Muscle Soreness [24 hours]
Subjective muscle soreness will be quantified using a visual analog scale on trial day mornings and evenings as well as before and after exercise.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Healthy, recreationally active, male and female participants.
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Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
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Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
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Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
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Participants will be aged 18-35 years old.
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Participants must be normal to overweight (e.g., BMI 18.5-30).
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Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
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Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
Exclusion Criteria:
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Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
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Self-reported regular tobacco use.
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Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
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Regular use of anti-inflammatory drugs (e.g., ibuprofen).
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Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
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Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
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Use of birth control and discontinued use in the last 3-months (female only).
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Goldring Centre for High Performance Sport at the University of Toronto | Toronto | Ontario | Canada | M5S 2C9 |
Sponsors and Collaborators
- University of Toronto
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- CVC