Acute Effect of Fructose on Lipid Metabolism and Gender Differences

Sponsor

University of Lausanne (Other)

Overall Status

Completed

CT.gov ID

NCT00620360

Collaborator

(none)

Enrollment

Location

Arm

Duration (Months)

1.6

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

It has been widely documented that fructose overfeeding increases plasma triglycerides and hepatic de novo lipogenesis, and impairs insulin sensitivity in healthy male volunteers. The effect of gender on the metabolic responses to fructose remains an important open question, however.

The objective of this study is to compare the effect of an acute oral fructose load on carbohydrate and lipid metabolism in healthy young males and females.

Condition or Disease	Intervention/Treatment	Phase
Lipid Metabolism	Dietary Supplement: fructose Dietary Supplement: Fructose	N/A

Detailed Description

The study is aimed at comparing the effects of oral fructose on several specific metabolic pathways in males and females.Participants will receive an isoenergetic diet containing 55% carbohydrate, 15% protein and 35% lipids for three days prior to testing. After this period of controlled diet, they will be studied for 2 hours in the post-absorptive state (Time 0-120 min) and over a 6 hours period (Time 120-480 min) during which they will receive 4 loads of 0,30 g/kg fat free mass U-13C labelled fructose, at times 120, 180, 240, 300. Throughout the study, deuterated glucose and glycerol will be infused to monitor whole body glucose production and glycerol turnover.

The following parameters will be monitored in basal conditions and after the ingestion of the load of fructose:

Glycerol turnover(glycerol 2H5)
de novo lipogenesis (incorporation of 13C into palmitate of VLDLs)
whole body energy expenditure and net substrate oxydation (indirect calorimetry)
net fructose oxidation (breath 13CO2)
glucose turnover: (6,6 2H2 Glucose)
plasma glucose, free fatty acids, ketone bodies, lactate, insulin, triacylglycerol, total cholesterol, VLDL, LDL, and HDL subfractions

An adipose tissue (periumbilical subcutaneous) biopsy will be obtaine by needle aspiration under local anesthesia in fasting conditions (time 0 min) and after fructose (time 480 min) to assess the effects of fructose on adipose gene expression profile. Key genes involved in the regulation of carbohydrate (GLUT 4, hexokinase, PDH-kinase), lipid (FAT-CD36, FABP, acetylCoA carboxylase, malonyl-CoA decarboxylase, PPARg) and energy metabolism (PGC-1a, UCP2)will be monitored

Results obtained in males and females will be compared with two-way analysis of variance

Study Design

Study Type:

Interventional

Actual Enrollment :

18 participants

Allocation:

Non-Randomized

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Basic Science

Official Title:

Acute Effect of Fructose on Lipid Metabolism and Gender Differences

Study Start Date :

Jan 1, 2008

Actual Primary Completion Date :

Dec 1, 2008

Actual Study Completion Date :

Dec 1, 2008

Arms and Interventions

Arm	Intervention/Treatment
Experimental: fructose acute fructose administration	Dietary Supplement: fructose acute administration of 4 times 0.3g fructose/kg lean body mass Dietary Supplement: Fructose acute administration of 4 times 0.3 g/kg fat-free mass oral fructose

Arm

Intervention/Treatment

Experimental: fructose

acute fructose administration

Dietary Supplement: fructose

acute administration of 4 times 0.3g fructose/kg lean body mass

Dietary Supplement: Fructose

acute administration of 4 times 0.3 g/kg fat-free mass oral fructose

Outcome Measures

Primary Outcome Measures

Hepatic de novo lipogenesis [acute effect of dietary fructose (within 6 hours)]

Secondary Outcome Measures

Whole body lipid oxidation, glucose turnover, glycerol turnover, plasma substrates, hormone and energy expenditure (free fatty acid, glucose, lactate, beta-hydroxybutyrate, glycerol, VLDL- Triglycerides and insulin) expression of key adipose genes [acute effect of fructose (within 6 hours)]

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 35 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

Males and females
Age 18-35
Healthy
Body mass indexes (BMI) between 19 and 25 kg/m2
Informed consent obtained

Exclusion Criteria:

Smokers
Alcohol intake > 30g/day
Drug abuse
Diabetes mellitus

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Centre Hospitalier Universitaire Vaudois	Lausanne	Vaud	Switzerland	1005

Sponsors and Collaborators

University of Lausanne

Investigators

Principal Investigator: Luc Tappy, MD, University of Lausanne

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

Luc Tappy, MD, Professor of physiology, University of Lausanne

ClinicalTrials.gov Identifier:

NCT00620360

Other Study ID Numbers:

279/07/CE/FBM

First Posted:

Feb 21, 2008

Last Update Posted:

Feb 24, 2012

Last Verified:

Feb 1, 2012

Keywords provided by Luc Tappy, MD, Professor of physiology, University of Lausanne

hepatic de novo lipogenesis

Study Results

No Results Posted as of Feb 24, 2012