Is Lymphocyte Subtype Important for Acute Pancreatitis Severity?
Study Details
Study Description
Brief Summary
Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease.
In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis and lymphocyte subtypes with Flow-cytometry.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In patients with severe pancreatitis, aggressive fluid replacement, organ damage follow-up, appropriate antibiotherapy, and endoscopic sphincterotomy and radiological interventions may be of great benefit. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease. There are limited number of studies in the literature about the immune parameters in the evaluation of acute pancreatitis. In a studies, serum inflammatory markers such as IL-1, IL-6 and CD4, CD8 T lymphocyte and Treg population were evaluated.Treg cells are reported to be an independent prognostic factor in determining the severity of acute pancreatitis. In patients diagnosed with acute biliary pancreatitis, determination of the course of the disease at the time of diagnosis is extremely important for treatment and survival.
In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis with lymphocyte subtypes via Flow-cytometry.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Mild pancreatitis Patients with mild acute biliary pancreatitis according to Atlanta classification |
Diagnostic Test: Flow Cytometric analysis
Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups
|
| Moderate Pancreatitis Patients with moderate acute biliary pancreatitis according to Atlanta classification |
Diagnostic Test: Flow Cytometric analysis
Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups
|
| Severe pancreatitis Patients with severe acute biliary pancreatitis according to Atlanta classification |
Diagnostic Test: Flow Cytometric analysis
Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups
|
| Control Healthy volunteers |
Diagnostic Test: Flow Cytometric analysis
Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups
|
Outcome Measures
Primary Outcome Measures
- Lymphocyte subtypes 1 [9 months]
T helper (CD4) (%)
- Lymphocyte subtypes 2 [9 months]
T cytotoxic (CD8) (%)
- Lymphocyte subtypes 3 [9 months]
Treg (CD25 and CD127) (%)
Eligibility Criteria
Criteria
Inclusion Criteria:
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18-80 years old
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Diagnosis of acute biliary pancreatitis
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Sign the voluntary consent form
Exclusion Criteria:
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Being under 18 or older than 80
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Not signing the voluntary consent form
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Pancreatitis resulting from an interventional procedure (ERCP, surgery, etc.)
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Pregnant women
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Being a history of immunodeficiency
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Cancer history
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | İstanbul Training and Research Hospital | Istanbul | Turkey | 34098 | |
| 2 | Gaziosmanpaşa Taksim Eğitim ve Araştırma Hastanesi | Istanbul | Turkey | 34900 |
Sponsors and Collaborators
- Istanbul Training and Research Hospital
Investigators
- Principal Investigator: Oguzhagan Batikan, M.D., İstanbul Training and Research Hospital
Study Documents (Full-Text)
None provided.More Information
Publications
- Li J, Yang WJ, Huang LM, Tang CW. Immunomodulatory therapies for acute pancreatitis. World J Gastroenterol. 2014 Dec 7;20(45):16935-47. doi: 10.3748/wjg.v20.i45.16935. Review.
- Wang W, Xiang HP, Wang HP, Zhu LX, Geng XP. CD4 + CD25 + CD127 high cells as a negative predictor of multiple organ failure in acute pancreatitis. World J Emerg Surg. 2017 Feb 2;12:7. doi: 10.1186/s13017-017-0116-7. eCollection 2017.
- Yang Z, Zhang Y, Dong L, Yang C, Gou S, Yin T, Wu H, Wang C. The Reduction of Peripheral Blood CD4+ T Cell Indicates Persistent Organ Failure in Acute Pancreatitis. PLoS One. 2015 May 4;10(5):e0125529. doi: 10.1371/journal.pone.0125529. eCollection 2015.
- Pancreatitis 01.06.2020