FLIO Group: Measurement of Retinal Auto Fluorescence With a Fluorescence Lifetime Imaging Ophthalmoscope
Study Details
Study Description
Brief Summary
Fluorescent lifetime microscopy has emerged as a useful tool to study fluorescent lifetimes in vitro. Fluorescence lifetime represents the average amount of time a fluorophore remains in the excited state following excitation and depends on the fluorophores molecular environment. Fluorescence lifetime ophthalmoscopy (FLIO) is a technique which can quantify fluorescence lifetimes in the human retina in vivo. The purpose of this study is to investigate fluorescence lifetime characteristics in the human retina by using a FLIO. The investigators hypothesize that FLIO will allow to identify areas of retinal metabolic stress such as ischemia by detecting changes in fluorescence lifetimes.
Condition or Disease | Intervention/Treatment | Phase |
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Detailed Description
Background
Ophthalmic imaging has made considerable progress in the last years. Especially the introduction of optical coherence tomography and the scanning laser ophthalmoscope has helped to understand the structural changes underlying various retinal diseases. However, the appearance of structural changes during retinal disease often represents irreversible functional loss with only limited treatment options. In order to prevent loss of vision, retinal diseases should ideally be diagnosed before structural changes occur. This can be achieved by imaging metabolic changes of the retina as most retinal diseases such as age related macular degeneration or diabetic retinopathy are associated with distinct metabolic changes, mainly related to oxidative stress.
Recently, a novel device for imaging fluorescent lifetimes of the retina in vivo, the fluorescent lifetime ophthalmoscope (FLIO), has been developed. This device is able to measure fluorescence lifetimes in the retina on a macroscopic level and may be able to shed new light on metabolic diseases of the retina.
Objective
To define fluorescence lifetime characteristics in healthy patients and patients with various retinal diseases.
Methods
The investigators will use a novel device, the fluorescence lifetime ophthalmoscope (FLIO) to investigate fluorescence lifetimes in the human retina.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
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Healthy patients Healthy patients |
Device: Fluorescence lifetime ophthalmoscope
All patients and healthy subjects will be imaged with the fluorescence lifetime ophthalmoscope
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Patients with various retinal diseases Various retinal diseases (vascular, hereditary, degenerative) |
Device: Fluorescence lifetime ophthalmoscope
All patients and healthy subjects will be imaged with the fluorescence lifetime ophthalmoscope
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Outcome Measures
Primary Outcome Measures
- Fluorescence lifetime measured by a fluorescence lifetime imaging ophthalmoscope [at baseline]
Measured by fluorescence lifetime variable (TAU). Measured once; in some patients, up to 4 measurements within 2 years will be done
Secondary Outcome Measures
- Repeatability of FLIO [at baseline]
Two independent measurements
Eligibility Criteria
Criteria
Inclusion Criteria:
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Subject must be willing to give written informed consent
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Healthy volunteers 18 years of age or greater
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Patients 18 years of age or greater
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No significant media opacities
Exclusion Criteria
- Opacities of ocular media excluding detailed observation of the retina
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
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1 | Department of Ophthalmology, Bern University Hospital, Bern, 3010 Bern, Switzerland | Bern 3010 | Switzerland | 3010 |
Sponsors and Collaborators
- University Hospital Inselspital, Berne
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- FLIO