Meteorin-like Levels in Individuals With Periodontitis
Study Details
Study Description
Brief Summary
Metrnl has proven to be an inflammatory-related immunoregulatory cytokine and shown to play a role in the pathogenesis of human inflammatory diseases. Metrnl is highly expressed from the oral mucosa to the esophagus. Periodontitis is a chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. The authors think that Metrnl may play a role in the periodontitis. The aim of this study is to compare the Metrnl, TNF-α, IL-1β, TGF-β, IL-10 and VEGF levels of healthy and periodontitis individuals.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Periodontitis is a destructive chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. Cytokines play an important role in inflammation that occurs in periodontal disease.
Tumor Necrosis Factor-α (TNF-α), Interleukine -1β ( IL-1β )and Interleukine-6 (IL-6), Prostaglandin E2 (PGE2) and Interferon gamma (IFN- γ) levels are associated with periodontal destruction in the pathogenesis of periodontal disease. Vascular endothelial growth factor (VEGF) are important features of inflammation, wound healing and bone healing. Cytokines such as Interleukine-10 (IL-10) and transforming growth factor- β (TGF-β) have been reported to decrease periodontal disease progression rate.
Gingival crevicular fluid shows the cellular response in the periodontium. The most important host-related ingredients are inflammatory markers, including cytokines, enzymes, and interleukins. Saliva analysis is used in the diagnosis of systemic conditions as well as in the diagnosis of oral pathologies.
Meteorin- like (Metrnl) is a protein that plays a role in natural and acquired immune-related functions and inflammatory responses, and expressed in barrier tissues (skin and mucosa) as well as various macrophage populations, from dendritic cells and some granulocytes. Metrnl has proven to be an inflammatory-related immunoregulatory cytokine and shown to play a role in the pathogenesis of human inflammatory diseases. Metrnl, which is highly expressed from the oral mucosa to the esophagus, may play a role in the onset of periodontitis and an effective marker in preventing the progression of destruction. We think that Metrnl plays an important role in periodontitis, an inflammatory disease. The aim of this study is to compare the Metrnl, TNF-α, IL-1β, TGF-β, IL-10 and VEGF levels of healthy and periodontitis individuals
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Healthy Individuals with Periodontally Healthy |
Other: Saliva
individuals' saliva samples will be collected and transferred to eppendorf tubes.
Until biochemical analysis is carried out will be stored in -80ºC.
Other Names:
Other: gingival crevicular fluid
GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.
Until biochemical analysis is carried out will be stored in -80ºC.
Other Names:
|
| Gingivitis Individuals with Gingival Inflammation |
Other: Saliva
individuals' saliva samples will be collected and transferred to eppendorf tubes.
Until biochemical analysis is carried out will be stored in -80ºC.
Other Names:
Other: gingival crevicular fluid
GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.
Until biochemical analysis is carried out will be stored in -80ºC.
Other Names:
|
| Periodontitis Individuals with Periodontitis |
Other: Saliva
individuals' saliva samples will be collected and transferred to eppendorf tubes.
Until biochemical analysis is carried out will be stored in -80ºC.
Other Names:
Other: gingival crevicular fluid
GCF samples of individuals will be collected by paper strips and transferred to eppendorf tubes.
Until biochemical analysis is carried out will be stored in -80ºC.
Other Names:
|
Outcome Measures
Primary Outcome Measures
- TNF-α [through study completion, an average of 6 months]
saliva and serum levels
- IL-1β [through study completion, an average of 6 months]
saliva and serum levels
- Metrnl [through study completion, an average of 6 months]
saliva and serum levels
- IL-10 levels [through study completion, an average of 6 months]
saliva and serum levels
- saliva VEGF [through study completion, an average of 6 months]
saliva and serum levels
- TGF-β [through study completion, an average of 6 months]
saliva and serum levels
Secondary Outcome Measures
- Plaque Index [up to 20 weeks]
Silness-Löe
- Probing Depth [up to 20 weeks]
millimeter
- Bleeding on Probe [up to 20 weeks]
Ainamo&Bay
Eligibility Criteria
Criteria
Inclusion Criteria:
-
systemically healthy
-
individuals who does not have a disease under treatment,
-
individuals without regular systemic medication
Exclusion Criteria:
-
smoking
-
receiving periodontal treatment in the last 6 months
-
pregnancy
-
menopausal or menstrual period
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Ebru Sağlam | İstanbul | Eyalet/Yerleşke | Turkey | 34668 |
Sponsors and Collaborators
- Istanbul Saglik Bilimleri University
Investigators
- Study Chair: Ayşe Toraman, Dr., Saglik Bilimleri Universitesi
- Study Chair: Serhat Köseoğlu, assoc prof, Saglik Bilimleri Universitesi
- Study Chair: Mehmet Sağlam, assoc prof, Izmir Katip Celebi University
- Study Chair: Levent Savran, PhD, Izmir Katip Celebi University
- Principal Investigator: Ebru Sağlam, Dr, Saglik Bilimleri Universitesi
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- Sağlık Bilimleri Üniversitesi