Foot-skin M&M: Foot-skin Microbiome and Metabolomics of Pitted Keratolysis
Study Details
Study Description
Brief Summary
This study aimed to investigate the skin microbiome and metabolomics of patients with pitted keratolysis.
Condition or Disease | Intervention/Treatment | Phase |
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N/A |
Detailed Description
Pitted keratolysis is a skin infectious disease which can cause an unpleasant smell that can affect self-confidence and interpersonal relationships. The research problem was initiated through a community outreach program organized by the Department of Dermatology Siriraj Hospital, Thailand. From many years of experiences, our dermatologist teams observed that pitted keratolysis was a common disease among naval cadets who wore enclosed footwear for long hours, and it was more prevalent after a boot camp that spanned over months. This disease is chronic with no efficient standard treatment and limited information on the pathogenesis of the disease. Therefore, we propose to conduct a study on the pathogenesis of pitted keratolysis and malodorous feet through a longitudinal study of foot-skin microbiome and associated volatile metabolomic profiles of foot odor. With the unique population, with similar age range, daily activities, and diet, this study will allow to focus on the association of changes in microbiome in relation to pathogenesis of pitted keratolysis and malodorous feet, with fewer cofounding factors.
The skin microbiome is a group of various microorganisms, such as bacteria, fungi, and viruses, residing on skin. Human skin microbiome can differ with respect to age, ethnicity, diet, climate and environment. Recently, there have been numerous studies highlighting the association of microbes with pathogenesis of various skin diseases, such as atopic dermatitis, acne vulgaris, and psoriasis (references?). In this project, we hypothesize that the alteration of skin microbiome as well as changes in metabolite profiles can be associated with the mechanism of the disease. The skin microbiome data generated in this study will be analyzed for microbial profiles using the QIIME2, a next-generation microbiome bioinformatics platform, and a graphic software package, "Statistical Analysis of Taxonomic and Functional Profiles" (STAMP). In parallel, we will apply a high throughput gas chromatography mass-spectrometry-based metabolomics to detect volatile metabolites present in foot odor in association with the observed foot-skin microbiome. This metabolite information will not only provide an additional aspect of pathogenesis of the disease but also might offer a new standard indicator of foot odor that can be further used in clinical practice. Finally, skin microbiome and metabolomic data will be analyzed using various methods of correlation analysis as well as integrative analysis to gain deeper understanding of the disease mechanism at the molecular levels.
The outcomes of this project will provide insights into the pathogenesis of pitted keratolysis. The scientific knowledge gained from the study will be translated into a more effective treatment of the disease. The human microbiome of healthy population and the disease-specific microbiome data will lay the groundwork to develop a human microbiome database for Thai people. Additionally, the success of the project will allow the development of advanced instrumental methodology and an integrative analysis approach to analyze multi -omics data, which will be great assets in advancing multidisciplinary research.
Study Design
Arms and Interventions
Arm | Intervention/Treatment |
---|---|
No Intervention: Normal feet Swab their feet to analyze foot microbiome via 16s DNA sequencing Collect their socks to analyze metabolomics via gas chromatography |
|
Experimental: Foot odor without pitted keratolysis Swab their feet to analyze foot microbiome via 16s DNA sequencing Collect their socks to analyze metabolomics via gas chromatography Apply 4% chlorhexidine for 1 month Re-evaluate their feet at time of treatment finish and 2 months after treatment finish. The forementioned methods (swab and collect their socks) will be applied on their feet again. The microbiome and metabolomics will be analyzed to evaluate the difference of microbe and substances after treatment. |
Drug: 4%Chlorhexidine
4%Chlorhexidine was given to participants with foot odor for 1 month.
|
Experimental: Foot odor with pitted keratolysis Swab their feet to analyze foot microbiome via 16s DNA sequencing Collect their socks to analyze metabolomics via gas chromatography Apply 4% chlorhexidine for 1 month Re-evaluate their feet at time of treatment finish and 2 months after treatment finish. The forementioned methods (swab and collect their socks) will be applied on their feet again. The microbiome and metabolomics will be analyzed to evaluate the difference of microbe and substances after treatment. |
Drug: 4%Chlorhexidine
4%Chlorhexidine was given to participants with foot odor for 1 month.
|
Outcome Measures
Primary Outcome Measures
- Amount of skin microbiome [3 months]
Foot-skin microbial profiles of healthy Thai people and patients with pitted keratolysis
- Composition of microbiota at skin [3 months]
Foot-skin microbial profiles of healthy Thai people and patients with pitted keratolysis
Secondary Outcome Measures
- Amount of metabolite in foot odor [3 months]
Metabolite profiling of foot odor
Eligibility Criteria
Criteria
Inclusion Criteria:
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Naval rating cadets at Chumpol Naval Rating School, Thailand who volunteer to participate in this study. Informed consent will be obtained from all participants.
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Age ≥ 18 years.
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No underlying disease.
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Normal BMI (18.5-22.9 kg/m2).
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No previous history of pitted keratolysis.
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No previous history of dermatophytosis complex.
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Refrain from washing their feet for 12 hours prior to sampling.
Exclusion Criteria:
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Volunteers who have taken medication or topical antibiotics for pitted keratolysis within 2 weeks before the enrollment.
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Volunteers who have taken oral antibiotics within 2 weeks before enrollment.
Contacts and Locations
Locations
Site | City | State | Country | Postal Code | |
---|---|---|---|---|---|
1 | Department of Dermatology Siriraj Hospital | Bangkoknoi | Bangkok | Thailand | 10700 |
2 | Department of Dermatology Siriraj Hospital | Bangkok | Thailand | 10700 |
Sponsors and Collaborators
- Mahidol University
Investigators
- Principal Investigator: Sumanas Bunyaratavej, MD, Mahidol University
Study Documents (Full-Text)
None provided.More Information
Publications
- Bolyen E, Rideout JR, Dillon MR, Bokulich NA, Abnet CC, Al-Ghalith GA, Alexander H, Alm EJ, Arumugam M, Asnicar F, Bai Y, Bisanz JE, Bittinger K, Brejnrod A, Brislawn CJ, Brown CT, Callahan BJ, Caraballo-Rodríguez AM, Chase J, Cope EK, Da Silva R, Diener C, Dorrestein PC, Douglas GM, Durall DM, Duvallet C, Edwardson CF, Ernst M, Estaki M, Fouquier J, Gauglitz JM, Gibbons SM, Gibson DL, Gonzalez A, Gorlick K, Guo J, Hillmann B, Holmes S, Holste H, Huttenhower C, Huttley GA, Janssen S, Jarmusch AK, Jiang L, Kaehler BD, Kang KB, Keefe CR, Keim P, Kelley ST, Knights D, Koester I, Kosciolek T, Kreps J, Langille MGI, Lee J, Ley R, Liu YX, Loftfield E, Lozupone C, Maher M, Marotz C, Martin BD, McDonald D, McIver LJ, Melnik AV, Metcalf JL, Morgan SC, Morton JT, Naimey AT, Navas-Molina JA, Nothias LF, Orchanian SB, Pearson T, Peoples SL, Petras D, Preuss ML, Pruesse E, Rasmussen LB, Rivers A, Robeson MS 2nd, Rosenthal P, Segata N, Shaffer M, Shiffer A, Sinha R, Song SJ, Spear JR, Swafford AD, Thompson LR, Torres PJ, Trinh P, Tripathi A, Turnbaugh PJ, Ul-Hasan S, van der Hooft JJJ, Vargas F, Vázquez-Baeza Y, Vogtmann E, von Hippel M, Walters W, Wan Y, Wang M, Warren J, Weber KC, Williamson CHD, Willis AD, Xu ZZ, Zaneveld JR, Zhang Y, Zhu Q, Knight R, Caporaso JG. Author Correction: Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol. 2019 Sep;37(9):1091. doi: 10.1038/s41587-019-0252-6.
- Grice EA, Segre JA. The skin microbiome. Nat Rev Microbiol. 2011 Apr;9(4):244-53. doi: 10.1038/nrmicro2537. Review. Erratum in: Nat Rev Microbiol. 2011 Aug;9(8):626.
- Holmes E, Wilson ID, Nicholson JK. Metabolic phenotyping in health and disease. Cell. 2008 Sep 5;134(5):714-7. doi: 10.1016/j.cell.2008.08.026.
- Iebba V, Totino V, Gagliardi A, Santangelo F, Cacciotti F, Trancassini M, Mancini C, Cicerone C, Corazziari E, Pantanella F, Schippa S. Eubiosis and dysbiosis: the two sides of the microbiota. New Microbiol. 2016 Jan;39(1):1-12. Review.
- Melnik BC. Linking diet to acne metabolomics, inflammation, and comedogenesis: an update. Clin Cosmet Investig Dermatol. 2015 Jul 15;8:371-88. doi: 10.2147/CCID.S69135. eCollection 2015. Review.
- Musthaq S, Mazuy A, Jakus J. The microbiome in dermatology. Clin Dermatol. 2018 May - Jun;36(3):390-398. doi: 10.1016/j.clindermatol.2018.03.012. Epub 2018 Mar 10.
- Nordstrom KM, McGinley KJ, Cappiello L, Zechman JM, Leyden JJ. Pitted keratolysis. The role of Micrococcus sedentarius. Arch Dermatol. 1987 Oct;123(10):1320-5.
- Patti GJ, Yanes O, Siuzdak G. Innovation: Metabolomics: the apogee of the omics trilogy. Nat Rev Mol Cell Biol. 2012 Mar 22;13(4):263-9. doi: 10.1038/nrm3314. Review.
- Perez Perez GI, Gao Z, Jourdain R, Ramirez J, Gany F, Clavaud C, Demaude J, Breton L, Blaser MJ. Body Site Is a More Determinant Factor than Human Population Diversity in the Healthy Skin Microbiome. PLoS One. 2016 Apr 18;11(4):e0151990. doi: 10.1371/journal.pone.0151990. eCollection 2016.
- Sander MA, Sander MS, Isaac-Renton JL, Croxen MA. The Cutaneous Microbiome: Implications for Dermatology Practice. J Cutan Med Surg. 2019 Jul/Aug;23(4):436-441. doi: 10.1177/1203475419839939. Epub 2019 Apr 2. Review.
- Schoch JJ, Monir RL, Satcher KG, Harris J, Triplett E, Neu J. The infantile cutaneous microbiome: A review. Pediatr Dermatol. 2019 Sep;36(5):574-580. doi: 10.1111/pde.13870. Epub 2019 Jul 23. Review.
- Singh G, Naik CL. Pitted keratolysis. Indian J Dermatol Venereol Leprol. 2005 May-Jun;71(3):213-5. Review.
- Szabó K, Erdei L, Bolla BS, Tax G, Bíró T, Kemény L. Factors shaping the composition of the cutaneous microbiota. Br J Dermatol. 2017 Feb;176(2):344-351. doi: 10.1111/bjd.14967. Epub 2017 Jan 23. Review.
- Thomas CL, Fernández-Peñas P. The microbiome and atopic eczema: More than skin deep. Australas J Dermatol. 2017 Feb;58(1):18-24. doi: 10.1111/ajd.12435. Epub 2016 Jan 28. Review.
- Yu Y, Dunaway S, Champer J, Kim J, Alikhan A. Changing our microbiome: probiotics in dermatology. Br J Dermatol. 2020 Jan;182(1):39-46. doi: 10.1111/bjd.18088. Epub 2019 Jul 28. Review.
- PK microbiome and metabolomic