Micro and Nanoplastics in Greenhouse Workers: Biomarkers of Exposure and Effect
Study Details
Study Description
Brief Summary
MNPLs effects on human health are still preliminary. Workers exposed to plastic residues are exposed constantly to MNPLs. One of these jobs corresponds to greenhouse workers, that could be considered as a population highly exposed to plastics. The objective of the research is to be able to detect MNPLs on body fluids as well as their potential genotoxic and immunological damage.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
The exponential increase in the production/use of plastic translates into a parallel increase of environmental plastic waste that is continuously degraded into MNPLs. Information on the MNPLs' effects on human health is still preliminary and, furthermore, the limitations in current methodologies prevent accurate exposure/risk assessment.
This observational study will obtain body samples of greenhouse workers and from a control group. That would allow optimizing specific biomarkers of human MNPLs exposure and the characterization of biomarkers of early effect that allows an association among the exposure to MNPLs and effects on human health.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Control Biomonitoring studies to detect MNPLs in biological samples and study of health effects. Determination of MNPLs levels, genotoxic damage, and immunological effects |
Other: Biomonitoring of MNPLs in biological samples of greenhouse workers and controls
Detection of biomarkers of exposure and genotoxic effect in biological blood, urine, exhalated air condensate, and feces
|
| Greenhouse workers Biomonitoring studies to detect MNPLs in biological samples and study of health effects. Determination of MNPLs levels, genotoxic damage, and immunological effects |
Other: Biomonitoring of MNPLs in biological samples of greenhouse workers and controls
Detection of biomarkers of exposure and genotoxic effect in biological blood, urine, exhalated air condensate, and feces
|
Outcome Measures
Primary Outcome Measures
- Biomonitoring study to detect the presence of MNPLs in body fluids [Up to 1 year]
Micro and nanoplastics and/or derivatives would be evaluated
- Genotoxicity effects of exposure to MNPLs [Up to 1 year]
Micronucleus assay
- Genotoxicity effects of exposure to MNPLs [up to 1 year]
Comet assay
- Study of the effects of MNPLs on immune system [Up to 1 year]
Investigating the effect of MNPLs on the function of the immune system (signalling and activation) in vivo and in high-risk individuals
Secondary Outcome Measures
- Surrogate biomarkers after MNPLs exposure [Up to 1 year]
Plastic derivatives in body fluids
Eligibility Criteria
Criteria
Inclusion Criteria of workers exposed to plastic residues:
-
Working in an operating greenhouse for a period of 1 month or more
-
Age over 18 years old
-
Absence of chronic pathology including neoplasia
-
Absence of infectious disease at the date of sample collection and for a period of more than 2 weeks
-
Spanish residence
Inclusion Criteria of controls :
-
Age over 18 years old. Matched by age, gender, and lifestyle to the exposed group.
-
Absence of chronic pathology including neoplasia
-
Absence of infectious disease at the date of sample collection and for a period of more than 2 weeks
-
Spanish residence
Exclusion Criteria of workers exposed to plastic residues:
-
Participation in another clinical study
-
Pregnancy
-
Unsigned informed consent
Exclusion Criteria of controls:
-
Participation in another clinical study
-
Pregnancy
-
Unsigned informed consent
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Ricard Marcos
- Instituto Murciano de Investigación Biosanitaria Virgen de la Arrixaca
- Hospital Universitario Virgen de la Arrixaca
Investigators
- Principal Investigator: Pablo Pelegrín, Instituto Murciano de Investigación Biosanitaria (IMIB)
- Study Chair: Juan Bernardo Cabezuelo, Hospital Clínico Universitario Virgen de Arrixaca (Murcia)
- Study Chair: Laura Martínez, Hospital Clínico Universitario Virgen de Arrixaca (Murcia)
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 2-1