miR-155 Expression in Episodic and Chronic Migraine

Sponsor

IRCCS National Neurological Institute "C. Mondino" Foundation (Other)

Overall Status

Recruiting

CT.gov ID

NCT05891808

Collaborator

(none)

Enrollment

Location

39.3

Anticipated Duration (Months)

2.3

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Migraine is a common, yet often disabling, neurological disease that affects over 1 billion people around the world. It's the second most disabling disease globally and the leading cause of disability for people under the age of 50, especially women. The effects of migraine aren't limited to the individual, with a tremendous economic impact on families, friends, and employers. To help reduce this burden, research is now focusing on developing biomarkers that can help with diagnosis, predicting response to treatments, and identifying those at risk of developing chronic migraine.

MicroRNAs (miRNAs) are one of the most promising classes, as they can modulate gene expression and affect a wide range of cellular processes. Other studies have already observed different miRNA expression in those with episodic migraine or chronic migraine, but no specific miRNAs have been identified as a strong and specific migraine signature. miRNA-155 is of particular interest, as it has been linked to inflammation and pain, and may be a potential target for migraine treatments.

It is known that the immune system plays a role in migraine headaches. Monocytes, a type of immune cell, may be involved in the development of migraines. Certain medicines, such as aspirin, can affect monocyte function and have been used to treat migraines. Recent research has also shown that microRNAs can regulate the activity of these cells and influence inflammation, which may be linked to migraine attacks.

This study aims to investigate the role of miRNA-155 and monocyte differentiation in migraine patients, and in particular its association with migraine phenotype and severity. We aim to study three groups of subjects: Episodic migraine (EM), Chronic migraine with or without Medication Overuse Headache (CM-MOH) and Healthy Controls (HCs).

Condition or Disease	Intervention/Treatment	Phase
Migraine Headache Pain Chronic Migraine

Detailed Description

Background:

Depending on the median frequency of headache, migraine is defined as episodic migraine (EM) (for those who have less than 15 migraine days per month), or chronic migraine (for those who have at least 15 headache days per month, 8 of which with migraine features, for at least three months). The chronification of migraine is often associated with a progressive increase of acute medications intake, leading to a condition known as Medication Overuse Headache (CM-MOH).

So far, there are no validated and reliable biomarkers of migraine, but the headache scientific community is intensely investigating the neurobiological signatures of migraine. Validated biological biomarkers may allow several steps forward in the management of migraine by: (I) refining the diagnosis according to biological features; (II) predicting the response to advanced therapies; (III) predicting which patients are at the greatest risk of migraine chronification and mark different phases of the migraine cycle; (IV) the identification of novel molecular targets for drugs development. Fulfilling these purposes, a class of molecules that has recently gained enormous interest are microRNAs (miRNAs). miRNAs are small endogenous noncoding RNAs that are around 22 nucleotides in length. miRNAs operate as post-transcriptional regulator of gene expression by promoting messenger RNA (mRNA) degradation or repressing mRNA translation. The regulation process performed by miRNAs is complex and articulated since an individual miRNA might target hundreds of different mRNAs, and conversely, each mRNA may be regulated by multiple miRNAs. It has been estimated that more than 60% of all protein-coding genes are regulated by miRNAs which consequentially determine the pleiotropic modulation of a wide variety of cellular processes involving differentiation, development and signaling. miRNAs are involved in the generation and maintenance of pain and several evidence suggest that specific miRNAs could play a role in migraine.

The micro-MIG study corroborated the hypothesis that different miRNAs expression is altered in EM and CM when compared to a control group, although no specific miRNAs were identified as a strong and specific migraine molecular signature.

miRNA-155 can be considered as a master regulator of inflammation since its expression can modulate many inflammatory stimuli such as: interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-α), alarmins (eg, IL-1α), hypoxia, nuclear factor erythroid 2-related factor 2 (NRF2), pathogen-associated and damage-associated molecular patterns as well as to toll-like receptor ligand (TLR) in various cell types. Recently, several lines of preclinical evidence further highlighted the role of miRNA-155 in inflammation and pain generation and maintenance also as a feasible therapeutic target. The inhibition of miRNA-155 can alleviate hyperalgesia and bone cancer pain as well as neuropathic pain in in-vivo rodent models. Therefore, it can be inferred that miRNA-155 might also play an important role in neurogenic inflammation, a process supposed to take place in the trigeminovascular system, and related to the pathogenic mechanisms underlying migraine pain.

Monocytes are immune system components which may play a role in migraine. For example, abortive migraine treatment may influence monocyte function. Acetylsalicylic acid inhibits monocyte chemotaxis, whereas metoprolol, metoclopramide, dihydroergotamine, and sumatriptan have no impact. microRNAs have been shown to regulate macrophage polarization and subsequent effects on inflammation.

Aims:

In order to shed light upon the mediators involved in migraine pathophysiology and its chronification, we aim to asses the different expression of miRNA-155 (in monocytes) in patients with EM, CM-MOH and healthy controls (HCs). Secondly, we aim to provide information about distribution of monocyte phenotype in the three groups.

Expected evaluations:

For all patients is scheduled a single evaluation over time - baseline (T0) - in the interictal migraine phase, during which they will undergo:

informed consent signing
screening visit
clinical and demographic data collection (based on revision of a headache diary)
venous blood sampling All patients will be evaluated in the morning, after night fasting, and in the inter-ictal migraine phase.

Detailed description of the methods planned for the biochemical analyses could be found here:

10.1186/s10194-020-01189-0;
10.1177/0333102420949201.

Sample size calculation:

Sample size was calculated from preliminary data available, which showed a difference in miR-155 levels in peripheral mononuclear cells of 1.69 RQ (relative quantification), with standard deviations of 1.15, and 2.69 RQ in healthy control and episodic migraine subjects, respectively. This difference has been assumed the same between episodic and chronic subjects.

Therefore, assuming a statistical power of 80 percent, a significance level of 95 percent and performing Bonferroni correction, we need a sample size of 24 subjects for the three experimental groups (HCs, CM-MOH and EM). Considering possible variability in dosing, we will enroll at least 30 subjects per group.

Pre-planned statistical analysis:

The difference between of miR155 expression among CM-MOH, EM and HCs will be determined by parametric (ANOVA) or non-parametric (Kruskal-Wallis) tests depending on the distribution of data. Parametric or non-parametric correlations will be used to measure the relationship between biochemical variables and clinical variables in CM-MOH and EM patients. Multivariate analyses will be performed according to the results of the univariate analysis. Statistical significance will be set at the 5% level (p<0.05).

Study Design

Study Type:

Observational

Anticipated Enrollment :

90 participants

Observational Model:

Case-Control

Time Perspective:

Cross-Sectional

Official Title:

Expression of miR-155 in Migraine: Association With Different Phenotypes and Disease Severity

Actual Study Start Date :

Sep 22, 2020

Anticipated Primary Completion Date :

Jan 1, 2024

Anticipated Study Completion Date :

Jan 1, 2024

Arms and Interventions

Arm	Intervention/Treatment
Healthy Controls Healthy subjects without present or past history of headache according to ICHD-III criteria.
Episodic Migraine Patients with a diagnosis of migraine without or with aura, with episodic pattern, according to ICHD-III criteria.
Chronic Migraine Patients with a diagnosis of chronic migraine with or without medication overuse headache according to ICHD-III criteria.

Outcome Measures

Primary Outcome Measures

Comparison of miR-155 expression in peripheral monocytes between the three study groups: chronic migraine, episodic migraine and healthy control. [Baseline (T0).]
miRNA-155 gene expression will be evaluated by real-time reverse transcription (RT-PCR). This assessment will be then normalized with U6 (a type of small nuclear RNA used as housekeeping gene) and expressed as Relative Quantification (RQ).

Secondary Outcome Measures

Cytofluorimetric evaluation of monocytes to determine their phenotype and subtype and the different colocalization of these between the three study groups: chronic migraine, episodic migraine and healthy control. [Baseline (T0).]
Phenotype evaluation: Cytofluorimetric assessment of the percentage of classical and non-classical M1 inflammatory (CD80+), and classical and non-classical M2 anti-inflammatory (CD163+) monocytes. Classical monocytes are characterized by high level expression of the CD14 cell surface receptor (CD14++ CD16- monocyte). Non-classical monocyte show low level expression of CD14 and additional co-expression of the CD16 receptor (CD14+CD16++ monocyte).
Comparison of gene expression in peripheral monocytes of pro-inflammatory (IL-1B, TNF-alpha) and anti-inflammatory (IL-10) cytokines between the three study groups: chronic migraine, episodic migraine and healthy control. [Baseline (T0).]
Cytokines genes expression will be evaluated by real-time reverse transcription (RT-PCR). This assessment will be then normalized with UBC (a type of small nuclear RNA used as housekeeping gene) and expressed as Relative Quantification (RQ).
Comparison of miR-382-5p and miR-34a-5p expressions in peripheral monocytes between the three study groups: chronic migraine, episodic migraine and healthy control. [Baseline (T0).]
miR-382-5p and miR-34a-5p expressions will be evaluated by real-time reverse transcription (RT-PCR). This assessment will be then normalized with U6 (a type of small nuclear RNA used as housekeeping gene) and expressed as Relative Quantification (RQ).

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 65 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Healthy Controls:

Inclusion Criteria:

age between >18 and <65 years, of both genders

Exclusion criteria:

diagnosis of primary and/or secondary headache according to ICHD-III criteria (only diagnosis of sporadic tension type headache is allowed)
diagnosis of neurological disorders
diagnosis of medical conditions considered clinically relevant by the researcher
pregnant and lactating women
taking NSAIDs, triptans or opiates in the previous 24 hours

Episodic Migraine

Inclusion Criteria:

diagnosis of migraine without or with aura according to ICHD-III criteria
age between >18 and <65 years of both genders
have completed a headache diary for at least 3 months at the time of enrollment

Exclusion criteria:

history of chronic migraine or other chronic headache subtypes according to ICHD-III criteria
concomitant diagnosis of other primary and/or secondary headache according to ICHD-III criteria (only diagnosis of sporadic tension headache is allowed)
diagnosis of other neurological disorders
diagnosis of medical conditions considered clinically relevant by the researcher
diagnosis of chronic pain syndrome of any nature
pregnant and lactating women
use of substances of abuse
taking NSAIDs, triptans or opiates in the previous 24 hours

Chronic Migraine

Inclusion Criteria:

diagnosis of chronic migraine with or without concomitant diagnosis of medication overuse headache according to ICHD-III criteria
history of chronic migraine for at least 1 year
age between >18 and <65 years, of both sexes
have completed a headache diary for at least 3 months at the time of enrollment

Exclusion criteria:

concomitant diagnosis of other primary and/or secondary headache according to ICHD-III criteria (only diagnosis of sporadic tension headache is allowed)
concomitant diagnosis of other neurological disorders
diagnosis of medical conditions considered clinically relevant by the researcher
diagnosis of chronic pain syndrome of any nature
pregnant and lactating women
use of substances of abuse
taking NSAIDs, triptans or opiates within the previous 24 hours