Examining The Effect of Blood Flow Restricted Aerobic Training on Mitochondrial Adaptations in Young Healthy Adults

• Sponsor: Queen's University (Other)
• Overall Status: Completed
• CT.gov ID: NCT04151095
• Collaborator: (none)
• Enrollment: 19
• Location: 1
• Arms: 2
• Actual Duration (Months): 7.3
• Patients Per Site Per Month: 2.6

Study Details

Study Description

Brief Summary

This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.

Condition or Disease	Intervention/Treatment	Phase
Mitochondrial Biogenesis	Other: BFR Exercise Training; Other: CTL Exercise Training	N/A

Study Design

• Study Type: Interventional
• Actual Enrollment: 19 participants
• Allocation: Randomized
• Intervention Model: Parallel Assignment
• Masking: Double (Investigator, Outcomes Assessor)
• Masking Description: Allocation concealment, assessor blinding
• Primary Purpose: Basic Science
• Official Title: Examining The Effect of Blood Flow Restricted Aerobic Training on Citrate Synthase Activity in Young Healthy Adults
• Actual Study Start Date: May 6, 2019
• Actual Primary Completion Date: Dec 13, 2019
• Actual Study Completion Date: Dec 13, 2019

Arms and Interventions

Arm	Intervention/Treatment
Experimental: BFR	Other: BFR Exercise Training; Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR).
Experimental: CTL	Other: CTL Exercise Training; Participants will complete exercise training on a recumbent bike placed on flat ground (CTL).

Outcome Measures

Primary Outcome Measures

1. Citrate Synthase [3 days prior to start of exercise training and 3 days after completing exercise training]

   Fold change in citrate synthase maximal activity

Secondary Outcome Measures

1. Transcription factor A, mitochondrial (TFAM) [3 days before training to 3 days after training]

   Fold change in TFAM protein

2. Nuclear respiratory factor, 1 (NRF1) [3 days before training to 3 days after training]

   Fold change in NRF-1 protein

3. Maximal oxygen consumption (VO2peak) [3 days before training to 3 days after training]

   Change in VO2peak (POST-PRE)

4. Heart rate (HR) [Every 5 minutes during each of the 7, 30-minute exercise sessions]

   Average HR

5. Revolutions per minute (RPM) [Every 5 minutes during each of the 7, 30-minute exercise sessions]

   Average RPM

6. Blood [lactate] [During all exercise sessions at 10 and 30 minutes]

   Average blood lactate concentration

7. Rating of perceived exertion (RPE) [Every 5 minutes during each of the 7, 30-minute exercise sessions]

   Average RPE

8. Nuclear Factor, Erythroid 2 Like 2 (NFE2L2) [3 days before training to 3 days after training]

   Fold change in NFE2L2

9. Cytochrome c oxidase (COX) [3 days before training to 3 days after training]

   Fold change in COX

10. Electromyography (EMG) [Throughout each of the 7, 30-minute exercise sessions]

    Median frequency and average amplitude of EMG signal

11. Near-infrared spectroscopy (NIRS) [Throughout each of the 7, 30-minute exercise sessions]

    Deoxygenated hemoglobin and oxygenated hemoglobin

Eligibility Criteria

Criteria

• Ages Eligible for Study: 18 Years to 30 Years
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: Yes

Inclusion Criteria:

• 3 hours of physical activity per week

• No previous cycling training

• No concurrent involvement in another training program

• Body mass index < 30 kg/m2

Exclusion Criteria:

• Presence of cardiovascular disease

• Presence of metabolic disease

• Taking regular oral medication

• Current smoker

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Queen's University	Kingston	Ontario	Canada	K7L3N6

Sponsors and Collaborators

• Queen's University

Investigators

• Principal Investigator: Brendon J Gurd, PhD, Queen's University

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

• Responsible Party: Brendon Gurd, PhD, Associate Professor, Queen's University
• ClinicalTrials.gov Identifier: NCT04151095
• Other Study ID Numbers: BFRT
• First Posted: Nov 5, 2019
• Last Update Posted: Sep 21, 2020
• Last Verified: Sep 1, 2020

• Individual Participant Data (IPD) Sharing Statement: Undecided
• Plan to Share IPD: Undecided
• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No
• Keywords provided by Brendon Gurd, PhD, Associate Professor, Queen's University: Mitochondria; Exercise training; VO2; Aerobic exercise; Citrate Synthase; Blood flow restriction

Study Results

No Results Posted as of Sep 21, 2020