Molecular Assessment of Oral Mutans Streptococcus Isolated From Different Patients

Sponsor

October University for Modern Sciences and Arts (Other)

Overall Status

Completed

CT.gov ID

NCT03802669

Collaborator

(none)

Enrollment

Location

4.2

Actual Duration (Months)

21.4

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

90 saliva samples will be isolated from patient who possess different ages and caries activity, attending outpatient clinic of October University of Modern Sciences and Arts. Then the determination of the best selective media for mutans streptococcus isolated from saliva and authenticate the MS via PCR technique.

Condition or Disease	Intervention/Treatment	Phase
Dental Caries	Other: oral mutans streptococcus

Study Design

Study Type:

Observational

Actual Enrollment :

90 participants

Observational Model:

Case-Control

Time Perspective:

Cross-Sectional

Official Title:

Molecular Assessment of Oral Mutans Streptococcus Isolated From Patients With Different Age and Caries Activity Via Selective Media and Protein Pattern.

Actual Study Start Date :

Jan 12, 2019

Actual Primary Completion Date :

Feb 15, 2019

Actual Study Completion Date :

May 20, 2019

Arms and Interventions

Arm	Intervention/Treatment
caries free children aged between 3-6	Other: oral mutans streptococcus quantitative assessment of mutans streptococcus of each group by using PCR and protein pattern
caries active children aged between 3-6	Other: oral mutans streptococcus quantitative assessment of mutans streptococcus of each group by using PCR and protein pattern
caries free children aged between 6-12	Other: oral mutans streptococcus quantitative assessment of mutans streptococcus of each group by using PCR and protein pattern
caries active children aged between 6-12	Other: oral mutans streptococcus quantitative assessment of mutans streptococcus of each group by using PCR and protein pattern
caries free adult aged between 18-25	Other: oral mutans streptococcus quantitative assessment of mutans streptococcus of each group by using PCR and protein pattern
caries active adult aged between 18-25	Other: oral mutans streptococcus quantitative assessment of mutans streptococcus of each group by using PCR and protein pattern

Outcome Measures

Primary Outcome Measures

oral mutans streptococcus screening [immediately]
screening of oral mutans streptococcus of each group to determine the differences

Eligibility Criteria

Criteria

Ages Eligible for Study:

3 Years to 25 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

healthy participant

Exclusion Criteria:

participant received antimicrobial therapy in previous month.
participant who taking xylitol chewing gum or undergo fluoride therapy.

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	October University of Modern Sciences and Arts	Cairo		Egypt

Sponsors and Collaborators

October University for Modern Sciences and Arts

Investigators

Principal Investigator: Naglaa Ezzeldin, lecturer, October University for Modern Sciences and Arts

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

Naglaa Ibrahim Ezz, Lecturer of pediatric dentistry department, faculty of dentistry, October University for Modern Sciences and Arts

ClinicalTrials.gov Identifier:

NCT03802669

Other Study ID Numbers:

ETH10

First Posted:

Jan 14, 2019

Last Update Posted:

Sep 17, 2019

Last Verified:

Sep 1, 2019

Individual Participant Data (IPD) Sharing Statement:

Plan to Share IPD:

Studies a U.S. FDA-regulated Drug Product:

Studies a U.S. FDA-regulated Device Product:

Keywords provided by Naglaa Ibrahim Ezz, Lecturer of pediatric dentistry department, faculty of dentistry, October University for Modern Sciences and Arts

Additional relevant MeSH terms:

Dental Caries

Study Results

No Results Posted as of Sep 17, 2019