Vitamin D2, Muscle Damage, NASCAR Pitcrew

Sponsor

Appalachian State University (Other)

Overall Status

Completed

CT.gov ID

NCT01825616

Collaborator

Dole Food Company (Industry)

Enrollment

Locations

Arms

Duration (Months)

Patients Per Site

3.6

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Hypothesis: Six weeks of supplementation with vitamin D (4000 IU/day) using Dole's Vitamin D Portobello Mushroom Powder will increase winter serum vitamin D levels, and improve muscle function and strength, and innate immunity (granulocyte/monocyte phagocytosis and oxidative burst activity), and attenuate exercise-induced muscle damage and DOMS.

Condition or Disease	Intervention/Treatment	Phase
Muscle Function Muscle Soreness Vitamin D Status	Dietary Supplement: Vitamin D2 mushroom powder Other: Placebo	N/A

Detailed Description

Vitamin D2 is found naturally in sun-exposed mushrooms, and vitamin D3 is synthesized in the skin when exposed to sunlight and is present in oil-rich fish such as salmon, mackerel, and herring. Mushrooms contain very little or any vitamin D2 but are abundant in ergosterol, which can be converted into vitamin D2 by ultraviolet (UV) illumination (Eur J Clin Nutr. 2011;65:965-71).

In recent decades, there has been increased awareness of the impact of vitamin D on muscle function (Scand J Med Sci Sports. 2010;20:182-90). In the early 20th century, athletes and coaches felt that ultraviolet rays had a positive impact on athletic performance, and evidence is accumulating to support this view. Both cross-sectional and longitudinal studies support a functional role for vitamin D in muscle, and the discovery of the vitamin D receptor in muscle tissue provides a mechanistic pathway for understanding the role of vitamin D within muscle. Studies in athletes have found that vitamin D status is variable and is dependent on outdoor training time (during peak sunlight), skin color, and geographic location (Pediatr Clin North Am. 2010;57:849-61).

25(OH)D is the best indicator and major form of vitamin D in the blood, with a circulating half-life of 2-3 weeks. Vitamin D deficiency is defined as a plasma vitamin D [25(OH)D] level of less than 20 ng/ml, with vitamin D insufficiency defined as 21-29 ng/ml. Estimates are that 20-100% of children, young and middle-aged adults, and community-dwelling elderly men and women are vitamin D deficient (J Clin Endocrinol Metab. 2011;96:1911-30).

Purpose: To determine if 6 weeks supplementation with Dole Vitamin D Portobello Mushroom Powder (4,000 IU/day) can, 1) increase winter serum vitamin D levels, and measure whether vitamin D supplementation 2) has a chronic influence on muscle function and strength, and innate immune function, and 3) can attenuate exercise-induced muscle damage and delayed onset of muscle soreness (DOMS) in athletes.

Study Design

Study Type:

Interventional

Actual Enrollment :

33 participants

Allocation:

Randomized

Intervention Model:

Parallel Assignment

Masking:

Double (Participant, Investigator)

Primary Purpose:

Prevention

Official Title:

Influence of 6-weeks Supplementation With Vitamin D Portobello Mushroom Powder on Muscle Function and Performance, Innate Immune Function, and Exercise-induced Muscle Damage and DOMS in NASCAR Pit Crew Members

Study Start Date :

Oct 1, 2012

Actual Primary Completion Date :

Jan 1, 2013

Actual Study Completion Date :

Jan 1, 2013

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Vitamin D2 mushroom powder 4000 IU/day vitamin D2 mushroom powder	Dietary Supplement: Vitamin D2 mushroom powder Subjects will be given Portobello mushroom powder with or without vitamin D mixed in soymilk powder in six plastic containers (one for each week of the study). Subjects will ingest one level teaspoon of the product each day (with or without 4,000 IU vitamin D2) and consume during breakfast in one of the following ways: mixed in water, juice, yogurt, or milk. Other Names: UV radiated Portobello mushroom powder
Placebo Comparator: Placebo Mushroom powder without vitamin D2 (not exposed to UV radiation)	Other: Placebo Mushroom powder without vitamin D2

Arm

Intervention/Treatment

Experimental: Vitamin D2 mushroom powder

4000 IU/day vitamin D2 mushroom powder

Dietary Supplement: Vitamin D2 mushroom powder

Subjects will be given Portobello mushroom powder with or without vitamin D mixed in soymilk powder in six plastic containers (one for each week of the study). Subjects will ingest one level teaspoon of the product each day (with or without 4,000 IU vitamin D2) and consume during breakfast in one of the following ways: mixed in water, juice, yogurt, or milk.

Other Names:

UV radiated Portobello mushroom powder

Placebo Comparator: Placebo

Mushroom powder without vitamin D2 (not exposed to UV radiation)

Other: Placebo

Mushroom powder without vitamin D2

Outcome Measures

Primary Outcome Measures

Muscle damage [6 weeks]
myoglobin (ug/L) and creatine kinase (IU/L) after eccentric resistance exercise
Muscle Function [6 weeks]
Bench press of body weight, reps to fatigue Vertical jump (watts( 300 yard shuttle run (seconds) 30-sec Wingate anaerobic power test (watts/kg)

Secondary Outcome Measures

Serum vitamin D concentration [6 weeks]
25(OH)D2 and 25(OH)D3
Immune Function [6 weeks]
White blood cell differential Myeloperoxidase Immune cell cytotoxicity

Eligibility Criteria

Criteria

Ages Eligible for Study:

20 Years to 40 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

NASCAR pit crew members
Willing to avoid food and supplement sources (during the 6-week supplementation period) that are high in vitamin D (specifically canned fish, cod liver oil, salmon, and supplements with high-dose vitamin D).
Agree to train normally.
Avoid the use of large dose vitamin/mineral supplements (above 100% recommended dietary allowances), and medications known to affect immune function.
Avoid the use of tanning beds.
Willing to adhere to all aspects of the study design.

Exclusion Criteria:

Not allergic to mushrooms.
Do not have a heart problem or have been told by your doctor not to engage in vigorous exercise.

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	ASU Human Performance Laboratory, North Carolina Research Campus	Kannapolis	North Carolina	United States	28081
2	ASU-NCRC Human Performance Laboratory	Kannapolis	North Carolina	United States	28081
3	ASU-NCRC Human Performance Lab	Kannapolis	North Carolina	United States	28081