The Effect of a Plant-blend Protein Isolate on Post-exercise Myofibrillar Protein Synthesis

• Sponsor: University of Exeter (Other)
• Overall Status: Completed
• CT.gov ID: NCT06129513
• Collaborator: (none)
• Enrollment: 10
• Location: 1
• Arms: 2
• Actual Duration (Months): 8.2
• Patients Per Site Per Month: 1.2

Study Details

Study Description

Brief Summary

Dietary protein ingestion is required to maximise the anabolic response during the recovery from resistance exercise. Whey protein is considered the optimal dietary strategy to maximise post-exercise muscle protein synthesis, but animal-protein production and consumption is associated with growing environmental and ethical concerns. Plant-based protein sources are considered of lesser anabolic quality than isonitrogenous boluses of animal-derived protein attributed to, at least in part, deficiencies in key essential amino acid. Blending different protein sources may overcome amino acid deficiencies and potentiate the post-exercise anabolic response. In the present study the investigators assessed the post-exercise muscle protein synthetic response following the ingestion of a novel plant-based protein isolate when compared with an isonitrogenous bolus of whey protein in healthy young, resistance trained women and men.

Condition or Disease	Intervention/Treatment	Phase
Muscle Metabolism	Behavioral: Bilateral resistance exercise; Dietary Supplement: Whey protein; Dietary Supplement: Plant-blend protein	N/A

Study Design

• Study Type: Interventional
• Actual Enrollment: 10 participants
• Allocation: Randomized
• Intervention Model: Crossover Assignment
• Masking: Double (Participant, Investigator)
• Primary Purpose: Basic Science
• Official Title: The Effect of a Novel Plant-blend Protein Isolate on Post-exercise Myofibrillar Protein Synthesis When Compared to Whey Protein in Healthy Young Adults
• Actual Study Start Date: Feb 24, 2022
• Actual Primary Completion Date: Oct 31, 2022
• Actual Study Completion Date: Oct 31, 2022

Arms and Interventions

Arm	Intervention/Treatment
Active Comparator: Whey protein isolate Following a bout of bilateral resistance exercise, participants will ingest 32 g protein from whey protein isolate	Behavioral: Bilateral resistance exercise; A bout of bilateral resistance exercise consisting of barbell back squat, leg leg press and leg extension; Dietary Supplement: Whey protein; Following execution of bilateral resistance exercise, participants will ingest 32 g protein from whey protein isolate
Experimental: Plant-blend isolate Following a bout of bilateral resistance exercise, participants will ingest 32 g protein from a novel plant-blend protein isolate	Behavioral: Bilateral resistance exercise; A bout of bilateral resistance exercise consisting of barbell back squat, leg leg press and leg extension; Dietary Supplement: Plant-blend protein; Following execution of bilateral resistance exercise, participants will ingest 32 g protein from plant-blend protein isolate

Outcome Measures

Primary Outcome Measures

1. Post-exercise myofibrillar protein synthesis following protein ingestion [4 hours]

   The fractional synthetic rate of myofibrillar proteins

Secondary Outcome Measures

1. Post-exercise myofibrillar protein synthesis during the early postprandial period [2 hours]

   The fractional synthetic rate of myofibrillar proteins

2. Post-exercise myofibrillar protein synthesis during the late postprandial period [2 hours]

   The fractional synthetic rate of myofibrillar proteins

3. Plasma amino acid response [4 hours]

   Post-exercise and postprandial change in plasma amino acid concentrations and availability

4. Serum insulin response [4 hours]

   Post-exercise and postprandial change in serum insulin concentrations

Eligibility Criteria

Criteria

• Ages Eligible for Study: 18 Years to 40 Years
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: Yes

Inclusion Criteria:

• BMI between 18 - 30

• Resistance trained (>3 times per week structured resistance exercise training for at least 3 months)

Exclusion Criteria:

• Any metabolic impairment

• Any cardiovascular impairment

• Smoking

• Lactose intolerance

• Allergies to products containing dairy, meat or nuts

• Prescribed intake of over the counter pharmaceuticals

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	University of Exeter	Exeter		United Kingdom

Sponsors and Collaborators

• University of Exeter

Investigators

None specified.

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

• Responsible Party: University of Exeter
• ClinicalTrials.gov Identifier: NCT06129513
• Other Study ID Numbers: 22-02-02-B-02
• First Posted: Nov 13, 2023
• Last Update Posted: Nov 13, 2023
• Last Verified: Nov 1, 2023

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No
• Keywords provided by University of Exeter: Myofibrillar Protein Synthesis; Resistance Exercise; Plant Protein; Stable Isotopes

Study Results

No Results Posted as of Nov 13, 2023