Neurogenic Inflammation in Peri-implant and Periodontal Diseases
Study Details
Study Description
Brief Summary
Regulatory effects of some neuropeptides substance-P (SP), neurokinin-A (NKA), calcitonin gene-linked peptide (CGRP) and neuropeptide-Y (NPY) )on inflammatory responses in periodontal disease has been described; however, the impact of neuropeptide levels are not clearly defined in healthy and diseased peri-implant tissues.ın order to evaluate the situation, thirty-nine implants that have been loaded over the past 12 months and their symmetrically matching teeth were evaluated using a split-mouth study design. Clinical periodontal examinations included the Silness-Löe plaque index, Löe-Silness gingival index, bleeding on probing, probing pocket depth and clinical attachment level parameters were determined. Gingival crevicular fluid (GCF)/Periimplant sulcular fluid (PISF) samples were collected, and the levels of the neuropeptides were determined by enzyme-linked immunosorbent assay. Correlations between GCF/PISF neuropeptide levels and the clinical examination parameters were evaluated in the peri-implant/periodontal soft tissues.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Group 1-Health periodontal This group created by individuals with healthy periodontal tissues. Periodontal status/peri-implant was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions. |
Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
|
| Group 2- Healthy peri-implant This group created by individuals with healthy peri-implant tissues. Periodontal status/peri-implant was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions. |
Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
|
| group 3- Gingivitis This group created by individuals with gingivitis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions. |
Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
|
| Group 4-Peri-implant Mucositis This group created by individuals with peri-implant mucositis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions. |
Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
|
| Group 5-Periodontitis This group created by individuals with periodontitis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions. |
Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
|
| Group 6-Periimplantitis This group created by individuals with peri-implantitis. Periodontal/peri-implant status was assessed by clinical examination and classified according to criteria proposed by the 1999 International World Workshop for a Classification of Periodontal Disease and Conditions. |
Diagnostic Test: gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
|
Outcome Measures
Primary Outcome Measures
- Gingival crevicular fluid/peri-implant sulcular fluid Level of Substance-P [8-10 am on the day following periodontal status assessment.]
Substance-P (SP) is a neuropeptide of the tachykinin family and marker of neurogenic inflammation.
- Gingival crevicular fluid/peri-implant sulcular fluid Level of neurokinin-A [8-10 am on the day following periodontal status assessment.]
Neurokinin-A is a neuropeptide of the tachykinin family and marker of neurogenic inflammation.
- Gingival crevicular fluid/peri-implant sulcular fluid Level of Calcitonin gene-related peptide [8-10 am on the day following periodontal status assessment.]
Calcitonin gene-related peptide (CGRP), another neuropeptide derived from peptidergic nerves and a marker of neurogenic inflammation.
Eligibility Criteria
Criteria
Inclusion Criteria:
The main inclusion criteria for patient selection were as follows:
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Compliance with systematic maintenance care;
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Adult, non-smoker, systemically healthy
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Not using any medicament for the last 6 months ;
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Partially dentated patient treated with implant-supported metal ceramic full crowns and/or fixed partial dentures
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As the study was split-mouth designed, in each individual at least one implant and one matching tooth in the same mouth symmetrically representing the same tooth group (premolars/molars);
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A single type of metal ceramic implant restoration that is functional for at least 1 year; and a matching tooth with no dental or endodontic restorations.
Exclusion Criteria:
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Medical history of cancer, rheumatoid arthritis, diabetes mellitus, or cardiovascular disease and any other systemic disease affecting lipid metabolism (i.e. impaired glucose tolerance, metabolic syndrome);
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Compromised immune system;
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Pregnancy, menopause, or lactation;
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Ongoing drug therapy that might affect the clinical characteristics of periodontitis and lipid metabolism;
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Dental treatment during the 6 months prior to data collection.
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Ondokuz Mayıs University
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- OMU KAEK 2011/752