NGAL and Hepcidin Levels in Hemodialysis Patients in Assiut University Hospital
Study Details
Study Description
Brief Summary
To assess the pattern of NGAL and Hepicidin in relation to anemia in End Stage Renal Disease Patients on regular hemodialysis in Assiut University Hospital and study the relation between both NGAL and Hepicidin.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Patients with chronic kidney disease have a chronic inflammatory state, due to many factors e.g. enhancedincidence of infections, the uremic milieu, elevated levels of proinflammatory cytokines, frequent presence of widespread arteriosclerosis, etc. Iron metabolism is disturbedin chronic inflammatory diseases.AlteredNGAL levels in hemodialysis patients was probablydue to the fact that this protein was involved in ironmetabolism and suggested that NGAL might be anew tool in the assessment of iron deficiency.Another study found in multipleregression analysis that; residual renal function, hepcidin,creatinine and hsCRP were predictors of serum NGAL inhemodialyzed patients. They concluded that NGAL is highly induced in dialyzed patients and could reflect both kidney function and iron metabolism.Several studies suggested that hepcidin plays a role as anegative regulator of intestinal iron absorption and ironrelease from macrophages. Hepcidin controls intestinal ironabsorption by regulating ferroportin expression on thebasolateral membrane of enterocytes .Hepicidin is also an acute phase reactant.Both NGAL and hepcidin are elevated inchronic kidney disease,not only through ironmetabolism, but both are also associated with inflammationand may be related to anemia. Possible relationship between NGAL and hepicidin is still under study.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Control healthy subjects without anemia. Adults > 18 years. Age and sex matched. No active infection or inflammation. |
Diagnostic Test: NGAL and Hepcidin
Both NGAL and hepcidin are elevated inchronic kidney disease,not only through iron metabolism, but both are also associated with inflammationand may be related to anemia
|
| ESRD with Hgb <11 g/dl. Adults > 18 years. ESRD patients on regular hemodialysis. Hgb < 11g/dl. No apparent infection or inflammation. |
Diagnostic Test: NGAL and Hepcidin
Both NGAL and hepcidin are elevated inchronic kidney disease,not only through iron metabolism, but both are also associated with inflammationand may be related to anemia
|
| ESRD with Hgb ≥ 11 g/dl Adults > 18 years. ESRD patients on regular hemodialysis. Hgb ≥ 11g/dl. No apparent infection or inflammation. |
Diagnostic Test: NGAL and Hepcidin
Both NGAL and hepcidin are elevated inchronic kidney disease,not only through iron metabolism, but both are also associated with inflammationand may be related to anemia
|
Outcome Measures
Primary Outcome Measures
- Assessment of Neutrophil Gelatinase Associated Lipocalin (NGAL) and Hepcidin levels in Hemodialysis patients in Assiut University Hospital, 60 patients and 12 controls will be recruited. [Bseline]
Assess the pattern of NGAL and Hepicidin in relation to anemia in End Stage Renal Disease Patients on regular hemodialysis in Assiut University Hospital and study the relation between both NGAL and Hepicidin.
Eligibility Criteria
Criteria
Inclusion Criteria:
For group 1:
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Adults > 18 years.
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Age and sex matched.
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No active infection or inflammation.
For group 2:
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Adults > 18 years.
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ESRD patients on regular hemodialysis.
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Hgb < 11g/dl.
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No apparent infection or inflammation.
For group 3:
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Adults > 18 years.
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ESRD patients on regular hemodialysis.
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Hgb ≥ 11g/dl.
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No apparent infection or inflammation.
Exclusion Criteria:
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Children and those >60 Years.
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Pregnant female.
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Patients with severe infection or with other immune system diseases.
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Assiut University
Investigators
- Study Chair: Walaa Hosny Hosny, Doctrate, Faculty of medicine - Assuit University
- Principal Investigator: Alaa Soliman Alaa Soliman, Doctrate, Faculty of medicine - Assuit University
Study Documents (Full-Text)
None provided.More Information
Publications
- Emans ME, Braam B, Diepenbroek A, van der Putten K, Cramer MJ, Wielders JP, Swinkels DW, Doevendans PA, Gaillard CA. Neutrophil gelatinase-associated lipocalin (NGAL) in chronic cardiorenal failure is correlated with endogenous erythropoietin levels and decreases in response to low-dose erythropoietin treatment. Kidney Blood Press Res. 2012;36(1):344-54. doi: 10.1159/000343392. Epub 2012 Dec 12.
- Malyszko J, Bachorzewska-Gajewska H, Malyszko JS, Koc-Zorawska E, Matuszkiewicz-Rowinska J, Dobrzycki S. Hepcidin - Potential biomarker of contrast-induced acute kidney injury in patients undergoing percutaneous coronary interventions. Adv Med Sci. 2019 Sep;64(2):211-215. doi: 10.1016/j.advms.2018.12.008. Epub 2019 Feb 26.
- Malyszko J, Malyszko JS, Kozminski P, Koc-Zorawska E, Mysliwiec M, Macdougall I. Possible relationship between neutrophil gelatinase-associated lipocalin, hepcidin, and inflammation in haemodialysed patients. Nephron Clin Pract. 2010;115(4):c268-75. doi: 10.1159/000313485. Epub 2010 Apr 28.
- Yılmaz I, Ozkok A, Kostek O, Kolukısa A, Duran I, Odabaş AR, Kemal Işman F, Işbilen Başok B. C-reactive protein but not hepcidin, NGAL and transferrin determines the ESA resistance in hemodialysis patients. Ren Fail. 2016;38(1):89-95. doi: 10.3109/0886022X.2015.1106896. Epub 2015 Nov 5.
- NGAL and Hepcidin in CKD