ZIP: Zinc in Potatoes Study
Study Details
Study Description
Brief Summary
Zinc is important in biology and allows the proper function of proteins in living organisms. Severe zinc deficiency in animals and humans over long periods of time can therefore cause adverse effects. In the UK, the zinc status of most people is adequate, but about 20% of the population, especially adolescents in deprived communities and vegetarians/vegans, are likely marginally zinc deficient. Because potatoes are a favoured food in adolescents and vegetarians/vegans, the investigators have improved the zinc content of Saxon potatoes by biofortification, which involves spraying potato plant leaves with zinc salts. The potato zinc concentration is about three times the level in unfortified potatoes of the same variety. This level of zinc can boost the zinc intake of people who are marginally zinc deficient so that they become zinc adequate. Indeed, in rat studies, the investigators have shown that addition of some zinc-biofortified potato to a low zinc diet improves the zinc and health status of the animals. In the present study, the investigators propose to investigate whether the potato biofortification can improve the zinc and health status of volunteers. Because most of the volunteers (healthy adult men and women after the menopause) might have normal or variable zinc status at recruitment, it might not be possible to see the benefits of the potato diets and therefore, the investigators shall reduce the zinc intake of all 45 participants to 1 mg Zn/d for a period of two weeks prior to feeding 15 randomly selected individuals the biofortified potato diets (4 mg Zn/d) for two weeks. Zinc and health status will be measured by blood tests before and after zinc depletion and after feeding the potato diets. Results will be compared with data from 15 volunteers eating unfortified potato diets with a daily placebo and 15 volunteers consuming the unfortified potato diets with a zinc supplement (18 mg/d) as a positive control.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
There is a small but critical window of dietary zinc intake from 1 to 4 mg Zn/day over which the symptoms of long-term zinc deficiency go from acute to chronic in nature. For example, at 1 mg Zn/day intake for several months, most people will develop skin lesions, but at 4 mg Zn/day, skin lesions would be very rare. However, impaired immune response can be detected when consuming zinc up to 6 mg/day over several months. Dietary intakes of <4 mg Zn/day in developed countries is rare but can be common in developing countries.
This study has been carefully designed to test the hypothesis that consumption of zinc biofortified potatoes can rescue the zinc status and health of people who are of low zinc status and consuming diets which are borderline acutely zinc deficient. The protocol for the study includes 2 weeks of zinc deprivation at around 1 mg Zn/day dietary intake (Very Low Zinc Diet) followed by 2 weeks of a borderline acutely/chronically deficient diet of around 4-5 mg Zn/day (Low Zinc Diet). Two weeks on the Very Low Zinc Diet has been carefully and deliberately selected to cause biochemical changes due to zinc deprivation but not signs and symptoms of deficiency such as skin lesions. Less than 2 weeks is insufficient to cause the biochemical effects and more that 2 weeks risks the physical manifestation of deficiency and also non-compliance by volunteers, since the diets may be less palatable than their habitual diet. Thus all volunteers will have low zinc status at the time of starting to eat the Low Zinc Diet, and the effect of eating the biofortified potatoes in addition, which will add an extra 1 mg to the daily zinc intake, should be seen in all the zinc and health status tests that the investigators are proposing. In rat studies in which the investigators did just this kind of intervention, the biofortified potato ameliorated the effect of zinc deficiency on growth, food intake and immune function.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Placebo Comparator: Unfortified potato plus placebo Volunteers given potato with no fortified zinc and a placebo |
Other: Unfortified potato plus placebo
Volunteers will be given 300g of zinc unfortified cooked potato as well as a placebo every day.
|
| Active Comparator: Zinc Biofortified potato plus placebo Volunteers given potato biofortified with zinc and a placebo |
Other: Zinc Biofortified potato plus placebo
Volunteers will be given 300g of cooked potato biofortified with zinc and a placebo every day.
|
| Active Comparator: Unfortified potato plus zinc supplement Volunteers given potato with no fortified zinc and a zinc supplement |
Other: Unfortified potato plus zinc supplement
Volunteers will be given 300g of zinc unfortified cooked potato and a zinc supplement containing 18mg of zinc, every day.
|
Outcome Measures
Primary Outcome Measures
- Plasma Zinc status [4 weeks]
Plasma zinc levels will be measured using ICP-MS
- Zinc status [4 weeks]
The Exchangeable Zinc Pool size (EZP) will be assessed by the analysis of the Zn stable isotopes excreted in the urine by ICP-MS/MS.
Secondary Outcome Measures
- Eye Electrophysiology [4 weeks]
After overnight dark adaptation using an eye patch, electroretinograms will be recorded following dilation with eye drops G. Tropicamide 1% & G. phenylephrine 2.5%) using non-invasive corneal electrodes.
- Thromboelastography (efficiency of blood clotting) [4 weeks]
The efficiency of blood clotting within participants will be assessed by collecting whole blood samples into 3.2% sodium citrate tubes and then analysing this for blood clotting parameters using thromboelastography (ROTEM). This analysis generates 4 values representing clot formation which will be assessed and compared between the different interventions: the reaction time (R), the speed of clot formation (K-value), the angle and the maximum amplitude (MA).
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Males aged 18 or over
-
Postmenopausal females
-
Healthy
-
BMI 19-35
Exclusion Criteria:
-
Physical or mental health problems
-
Long-term medication
-
Smoking
-
Alcohol >20 units/week
-
Health supplements
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | University of Aberdeen | Aberdeen | United Kingdom | AB25 2ZD |
Sponsors and Collaborators
- University of Aberdeen
- Fight for Sight
Investigators
- Principal Investigator: John H Beattie, PhD, University of Aberdeen
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 02/033/15