High Intensity Interval Training and Skeletal Muscle Insulin Sensitivity
Study Details
Study Description
Brief Summary
This human intervention study will test if 12 weeks of supervised HIIT-based intervention improves skeletal muscle NOGD capacity in obese subjects.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
19 overweight-obese (BMI => 27kg/m2), sedentary females and males aged 45-75yr will be enrolled in this study.
Participants will train 3 times/week under supervision during 12 weeks. Before, after and during this 12-week training period, there will be multiple metabolic measurements.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: HIIT This group of subjects will perform High Intensity Interval training 3x/week for 12 weeks |
Behavioral: High-intenstiy interval training
High-intensity interval training is a training of 30 minutes involving 10 bouts of 1 minute high intensity cycling (80-90% of maximum heart rate) interspersed by 2 minutes rest.
|
Outcome Measures
Primary Outcome Measures
- Non-oxidative glucose disposal [9 hours]
Measured during a 2-step hyperinsulinemic-euglycemic clamp combined with indirect calorimetry
Secondary Outcome Measures
- Skeletal muscle glycogen content [1 hour]
Assessed from skeletal muscle biopsies
- Skeletal muscle insulin sensitivity [9 hours]
Measured during a 2-step hyperinsulinemic-euglycemic clamp
- 24 hour glycaemic profile [48 hours]
continuous glucose monitor
- Skeletal muscle mitochondrial function assessed by Magnetic Resonance Spectroscopy Scan using 31P-MRS methodology, based on the phosphocreatine (PCr) recovery kinetics after exercise. [1 hour]
The participant is positioned in the scanner with a home-built exercise device to perform consecutive knee-extensions, in which the participant has to lift a weight whilst laying in the scanner (50-60% max leg capacity of the subjects) for 5 minutes. Scout images of the upper leg are acquired and fine-tuned shimming is applied. Before, during and after exercise, spectra are acquired every 4 seconds. The restoration of PCr is driven almost purely by oxidative metabolism, the time to restore the normal amount reflects mitochondrial function (a faster restoration time means better mitochondrial function).
- Metabolic Flexibility during exercise assessed by indirect calorimetry. [30 minutes]
Participants will undergo a standardized cycling test at low, moderate and high intensity (30%-50%-70%) during 10 minutes each. Throughout the test, indirect calorimetry will be performed in order to measure changes in substrate oxidation during exercise.
- Ectopic Fat accumulation in the liver assessed by magnetic resonance spectroscopy. [1 hour]
Participants will undergo a magnetic resonance spectroscopy scan from which hepatic fat content will be quantified. In the 1H-MRS spectra, the water signal, that is dominating the proton spectra, will be suppressed using frequency-selective pre-pulse and the spectra will be fitted to quantify the lipid peak. A separate spectrum will be measured without water suppression to quantify the unsuppressed water signal. The CH2/Water ratio will be used as parameter of intrahepatic lipid content.
- Maximal Acetylcarnitine formation in the upper-leg at rest and after exercise assessed by Magnetic Resonance Spectroscopy Scan [1 hour]
Magnetic resonance imaging (MRI) will be used to guide the spectroscopy measurements and fine shimming will be performed to optimize the magnetic field homogeneity within the region of interest. A volume of interest will be selected within the m. vastus lateralis from the MRI images and the 1H-MRS spectra will be acquired from this region of interest. The intensity of the creatine signal will be used as internal reference.
Other Outcome Measures
- Effect of consumption of insulinogenic, CHO-rich drink post-exercise [12 weeks]
Investigate whether the consumption of a insulinogenic, CHO-rich drink post-training prevention adverse effects related to blood glucose fluctuation while maintaining the effects of 12 weeks of HIIT on NOGD.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Signed informed consent
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Age 45 - 75 years old
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Overweight to obese (BMI => 27kg/m2)
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Sedentary - subjects do not perform any regular physical activity weekly(<3 times per week, <150 min/week).
Exclusion Criteria:
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Unstable body weight (weight gain or loss > 3 kg in the past three months)
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Participation in an intensive weight-loss program or in vigorous exercise program during the last year before starting the study.
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HbA1c > 6.5% and glucose clearance rate >350 ml/kg/min (by OGTT).
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Previously diagnosed with type 2 diabetes
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Active cardiovascular disease. This will be determined by the questionnaires and by screening on medication.
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Use of beta-blockers
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Anticoagulant therapy
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Systolic blood pressure >160 mmHg or diastolic blood pressure >100 mmHg
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Abuse of alcohol (> 3 units (1 unit = 10 gr ethanol) per day)
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Any contra-indication to Magnetic Resonance Imaging (MRI) scanning
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Participation in another biomedical study within 1 month before the first study visit, which may interfere with the outcomes of the present study.
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Use of any medication affecting the glucose homeostasis and whole body metabolism or diseases that may significantly interfere with the main aim of the study.
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Chronic renal dysfunction (creatinine >2 increased (normal value 64-104 µmol/l)
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Subjects who do not want to be informed about unexpected medical findings during the screening / study, or do not wish that their physician is informed, cannot participate in the study.
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Subjects will be included only when the dependent medical doctor of this study approves participation after evaluating all data obtained during the screening.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | University Maastricht | Maastricht | Limburg | Netherlands | 6229ER |
Sponsors and Collaborators
- Maastricht University
- Netherlands Organisation for Scientific Research
Investigators
- Principal Investigator: Matthijs Hesselink, Prof. PhD., Maastricht University
- Principal Investigator: Vera Schrauwen-Hinderling, PhD, Maastricht University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- NL62654.068.17