SURNUT: Metabolic Fate Modifications of Saturated Fats After an Overfeeding

Sponsor

Centre de Recherche en Nutrition Humaine Rhone-Alpe (Other)

Overall Status

Completed

CT.gov ID

NCT00905892

Collaborator

Institut National de la Santé Et de la Recherche Médicale, France (Other)

Enrollment

Location

53.9

Duration (Months)

0.7

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

The purpose of this study is to determine the partitioning of exogenous lipids in the postprandial period while a study of overfeeding. The method is based on the incorporation of a stable isotopic tracer (d31_palmitic acid, d31_C16) in lipoprotein triglycerides (TG-CHYLOMICRON and TG-VLDL) and in free fatty acids (FFA).

Condition or Disease	Intervention/Treatment	Phase
Obesity Lipid Metabolism Inflammation	Dietary Supplement: Hyperlipidic overfeeding	N/A

Detailed Description

Healthy overweight and lean young men are subjected to an overfeeding during 56 days which corresponds to a supplement of 761 Kcal/day. During two exploration days (before : D0 et after : D56 overfeeding) they have ingested a breakfast with tracer (d31_palmitic acid, d31_C16, 20mg/kg) and blood and urine samples were collected every hour of each exploration day. The enrichment in deuterium was measured by gas chromatography-organic mass spectrometry (GC-OMS) in palmitic acid pool of lipid fractions.

Study Design

Study Type:

Interventional

Anticipated Enrollment :

40 participants

Allocation:

N/A

Intervention Model:

Single Group Assignment

Masking:

None (Open Label)

Primary Purpose:

Basic Science

Official Title:

Metabolic Fate Modifications of Saturated Fats After an Overfeeding

Study Start Date :

Sep 1, 2005

Actual Primary Completion Date :

Dec 1, 2009

Actual Study Completion Date :

Mar 1, 2010

Outcome Measures

Primary Outcome Measures

Post prandial partitioning of exogenous lipids [Before and after overfeeding]

Secondary Outcome Measures

Genes expression in adipose and muscle tissues [Before and after overfeeding]

Eligibility Criteria

Criteria

Ages Eligible for Study:

18 Years to 55 Years

Sexes Eligible for Study:

Male

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

18 to 55 years old
BMI 18 to 30 kg/m²
Stable physical activity
Safety subject during medical consultation

Exclusion Criteria:

Medical or surgical history which may affect energy expenditure (renal -cardiovascular -hepatic- endocrine-inflammatory diseases)
Drug use that could affect energy expenditure (steroids, nicotine substitutes, thyroid hormones)
Eating disorder
Intensive sportive activity
Subjects who Smoke
Claustrophobic subjects