Epigenetic Effects of a Single Bout of Exercise on Cardiovascular Risk Factors and the Metabolome
Study Details
Study Description
Brief Summary
The purpose of this study is to determine the ability of acute exercise to regulate fat metabolism in muscle of overweight and obese people compared to lean people.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
After being informed about the study details and possible risks and benefits, 15 overweight/obese and 15 lean participants gave written informed consent and were then screened and enrolled in the study. All participants then gave a muscle sample and ate a calorically controlled breakfast. Participants completed ~1 hour of cycling exercise on a stationary bike and gave a second muscle sample 4 hours after eating breakfast.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Active Comparator: Lean males aged 18-30 years with BMI < 25 |
Behavioral: Acute exercise
All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.
|
| Experimental: Overweight/Obese males aged 18-30 years with BMI >= 25 |
Behavioral: Acute exercise
All participants consumed a standardized, high carbohydrate meal (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein) and completed an exercise testing trial, expending a total of 650 kcal upon completion 4h later.
|
Outcome Measures
Primary Outcome Measures
- Change from baseline in skeletal muscle acylcarnitines [Baseline and immediately after acute cycling exercise]
Metabolomics of skeletal muscle acylcarnitines is used as a measure of mitochondrial function by assessing long, medium and short chain acylcarnitine levels.
- Change from baseline in skeletal transcriptome [Baseline and immediately after acute cycling exercise]
RNA-seq is a method used to quantify changes in all known messenger RNA levels in the genome, giving an assessment of whole genome gene transcription changes.
- Change from baseline in skeletal muscle whole genome nucleosome maps [Baseline and immediately after acute cycling exercise]
MNase-seq is a method used to determine the occupancy/location of nucleosomes throughout the entire genome, supporting evidence of alterations in epigenetic modifications occurring at specific genomic loci that affect nucleosome occupancy/location and possibly gene transcription or silencing.
Secondary Outcome Measures
- Glucose [Baseline and immediately after acute cycling exercise]
Commercially validated and available colorimetric used to determine plasma glucose levels.
- Insulin [Baseline and immediately after acute cycling exercise]
Commercially validated and available ELISAs used to determine plasma insulin levels.
- Triglycerides [Baseline and immediately after acute cycling exercise]
Commercially validated and available colorimetric kits used to determine plasma triglyceride levels.
- Free fatty acids [Baseline and immediately after acute cycling exercise]
Commercially validated and available colorimetric kits used to determine plasma free fatty acid levels.
- Cholesterol [Baseline and immediately after acute cycling exercise]
Commercially validated and available colorimetric kits used to determine total cholesterol, HDL, LDL and VLDL levels in plasma.
Eligibility Criteria
Criteria
Inclusion Criteria:
- healthy males that completed a physical examination with a State Licensed M.D. or D.O. within the past 2 years and completed a physical activity readiness questionnaire (PARQ), the International Physical Activity Questionnaires (IPAQ) and and a medical history questionnaire prior to the study
Exclusion Criteria:
-
any participants that had not had a physical examination by a State Licensed M.D. or D.O. within the past 2 years
-
persons not considered "healthy" (with any previous medical diagnoses) or not medically cleared by a M.D. or D.O. to perform physical activity/exercise
-
persons who are regularly physically active
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Purdue University | West Lafayette | Indiana | United States | 47907 |
Sponsors and Collaborators
- East Carolina University
- Purdue University
- Louisiana State University Health Sciences Center Shreveport
Investigators
- Principal Investigator: Tara Henagan, PhD, Purdue University, Louisiana State University Medical School at Shreveport
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- AcuteExerciseNucleo