Oral and Systemic Levels of TFF-1 and TTF-3 in Periodontal Diseases
Study Details
Study Description
Brief Summary
Trefoil factor family (TFF) consists of a group of small peptides that have key roles in host immune response and repair of tissue damage. Interleukin (IL)-1β is a regulatory proinflammatory cytokine in periodontal inflammation. This study aimed to investigate the levels of TFF-1, TFF-3 and IL-1β in gingival crevicular fluid (GCF), saliva and serum of patients with gingivitis, stage 3 periodontitis and healthy individuals.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
A total of one hundred participants were enrolled for the study. All individuals were assessed clinically and radiographically. Full-mouth clinical periodontal examinations were performed by the measurements of probing depth (PD), clinical attachment level (CAL), gingival index (GI), plaque index (PI) and bleeding on probing (BOP). All these measurements were recorded at six sites around each tooth with a manual periodontal probe. The participants were categorized into three groups: healthy controls (n=25), patients with gingivitis (n=25) and patients with periodontitis (n=50). Gingival crevicular fluid, saliva and serum samples were collected from each participant one day after the clinical periodontal measurements. TFF-1, TFF-3 and IL-1β levels of these biofluids were determined using the enzyme-linked immunosorbent assay.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Healthy Healthy control group (n=25) consisted of the volunteers having clinically healthy gingiva, PD≤3 mm, BOP<10% and no sign of clinical attachment loss and radiographic alveolar bone destruction. |
Biological: biofluids
Gingival crevicular fluid, saliva and serum samples were obtained.
|
| Gingivitis Gingivitis group (n=25) had PD≤3 mm with BOP>50% in the entire mouth, and no clinical attachment loss or alveolar bone loss. |
Biological: biofluids
Gingival crevicular fluid, saliva and serum samples were obtained.
|
| Stage 3 periodontitis Stage 3 periodontitis group included the patients exhibiting PD ≥6 mm and interdental CAL ≥5 mm at %30 or more teeth. They had no more than four teeth loss. |
Biological: biofluids
Gingival crevicular fluid, saliva and serum samples were obtained.
|
Outcome Measures
Primary Outcome Measures
- Gingival crevicular fluid, saliva and serum TFF-1 and TFF-3 levels [one day after the clinical periodontal measurements]
Gingival crevicular fluid, saliva and serum TFF-1 and TFF-3 levels of gingivitis and periodontitis patients as well as healthy individuals were analyzed to identify the potential role of these peptides in periodontal diseases.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Systemically healthy individuals
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No history of smoking
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Individuals between the ages of 25-50 years
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Having at least 20 natural teeth
Exclusion Criteria:
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Any systemic disorders (i.e. diabetes mellitus, cardiovascular diseases, rheumatoid arthritis, immunological disorders, collagen-metabolic diseases, cancer)
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A history of smoking
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Pregnancy or lactation
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A history of surgical/non-surgical periodontal therapy in the past 1 year
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Drug therapy (e.g. anti-inflammatory, antibiotic treatment or any other pharmacological treatment) in the previous 6 months
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Zeynep Pinar Keles Yucel | Giresun | Turkey |
Sponsors and Collaborators
- Aydin Adnan Menderes University
Investigators
- Principal Investigator: Zeynep Pinar Keles Yucel, Giresun University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 2017/009