Effect of Acute Red Raspberry Consumption on Post-prandial Oxidative Stress

Sponsor

University of Toronto (Other)

Overall Status

Completed

CT.gov ID

NCT01242033

Collaborator

Unity Health Toronto (Other)

Enrollment

Location

Arms

Duration (Days)

8.7

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Eight healthy adult subjects will be given a meal of one, two or four cups red raspberries, or two slices white bread with or without 200 mg vitamin C, after an overnight fast and consumption of a low polyphenol diet for two days. Blood samples will be taken at various time points over an eight hour period to measure oxidative stress and antioxidant levels in the blood. Each subject will attend 5 study visits with one week intervals and be given each meal in a random order. It is hypothesized that raspberry consumption at higher doses will greater protect against meal-induced oxidative stress compared to bread controls.

Condition or Disease	Intervention/Treatment	Phase
Oxidative Stress Antioxidants	Dietary Supplement: red raspberries Dietary Supplement: bread controls	N/A

Study Design

Study Type:

Interventional

Actual Enrollment :

8 participants

Allocation:

Randomized

Intervention Model:

Crossover Assignment

Masking:

None (Open Label)

Primary Purpose:

Basic Science

Official Title:

An Acute Intervention Study Investigating the Effect of Red Raspberry Consumption on Post-prandial Oxidative Stress

Study Start Date :

Feb 1, 2010

Actual Primary Completion Date :

Mar 1, 2010

Actual Study Completion Date :

Mar 1, 2010

Arms and Interventions

Arm	Intervention/Treatment
Experimental: one cup red raspberries treatment meal consists of one cup red raspberries	Dietary Supplement: red raspberries single serving of one, two or four cups red raspberries
Experimental: two cups red raspberries treatment meal consists of two cups red raspberries	Dietary Supplement: red raspberries single serving of one, two or four cups red raspberries
Experimental: four cups red raspberries treatment meal consists of four cups red raspberries	Dietary Supplement: red raspberries single serving of one, two or four cups red raspberries
Placebo Comparator: bread treatment meal consists of two slices white bread	Dietary Supplement: bread controls two slices white bread alone or with 200 mg vitamin C supplement
Active Comparator: vitamin C treatment meal consists of two slices white bread and 200 mg vitamin C in the form of supplemental ascorbic acid	Dietary Supplement: bread controls two slices white bread alone or with 200 mg vitamin C supplement

Outcome Measures

Primary Outcome Measures

change in antioxidant capacity from baseline (-0.25 h) [-0.25, 0.25, 0.5, 1, 2, 4 hours post-intake]
plasma antioxidant capacity measured using the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation assay and expressed in Trolox Equivalents (TE)

Secondary Outcome Measures

change in lipid peroxidation from baseline (-0.25 h) [-0.25, 0.25, 0.5, 1, 2, 4 hours post-intake]
serum thiobarbituric acid reactive substances (TBARS) measured as an indicator of the lipid peroxidation product malondialdehyde (MDA)
change in thiols compared to baseline (-0.25 h) [-025, 0.25, 0.5, 1, 2, 4 hours post-intake]
plasma thiols quantified by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) assay as an indicator of the level of protection against protein oxidation

Eligibility Criteria

Criteria

Ages Eligible for Study:

20 Years to 30 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Yes

Inclusion Criteria:

healthy adults

Exclusion Criteria:

blood pressure greater than 120/80 mm Hg
body mass index greater than 24.9 kg/m^2
history of any chronic disease
currently taking medications
allergy to fruit

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital	Toronto	Ontario	Canada	M5C 2T2

Sponsors and Collaborators

University of Toronto
Unity Health Toronto

Investigators

Principal Investigator: A V Rao, PhD, University of Toronto

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

Responsible Party:

, ,

ClinicalTrials.gov Identifier:

NCT01242033

Other Study ID Numbers:

24774

First Posted:

Nov 16, 2010

Last Update Posted:

Dec 6, 2010

Last Verified:

Nov 1, 2010

Keywords provided by , ,

acute dietary intervention

Study Results

No Results Posted as of Dec 6, 2010