Protein Turnover in Healthy and Overuse-diseased Tendon
Study Details
Study Description
Brief Summary
The goal of this clinical trial is to examine the regional protein turnover in tendon tissue from patients with chronic patellar tendinopathy and comparing this to healthy controls. Further we will examine the effect of resistance training on protein turnover in healthy individuals.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: Chronic tendinopathy patients
|
Other: Cross-sectional comparison between healthy and tendinopathic group
Comparison of primary and secondary outcomes.
|
| Experimental: Healthy controls
|
Other: Progressive resistance training
Only the control group: 3 exercise bouts within the study period. Comparison between exercised and non-exercised leg.
Other: Cross-sectional comparison between healthy and tendinopathic group
Comparison of primary and secondary outcomes.
|
Outcome Measures
Primary Outcome Measures
- Protein turnover in tendon tissue from patients with chronic tendinopathy compared to healthy controls [8 days]
Fractional synthesis rate (FSR) measured in % pr. day in the trypsin-soluble and trypsin-insoluble fraction
Secondary Outcome Measures
- Tracer incorporation in isolated fractions of the tendon [8 days]
15N hydroxyproline enrichment measured in Mole percent Excess in the trypsin-soluble and trypsin-insoluble fraction
- Tracer incorporation in isolated fractions of the tendon [8 days]
15N proline enrichment measured in Mole percent Excess in the trypsin-soluble and trypsin-insoluble fraction
- Anterior-posterior dimensions of patella tendon [8 days]
Measured in millimeter
- Doppler flow in patella tendon [8 days]
Classified in grading system from 0-3.
- Subjective pain in patella tendon [8 days]
Questionnaire
- Subjective measure of physical activity and function [1 day]
Questionnaire
- Single-leg decline squat test [1 day]
Subjective pain on a scale from 0-10
- One leg knee extension strength test [8 days]
Only control-group.
- Leg press strength test [8 days]
Only control-group.
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Physical active men and women
-
Body mass index 18,5 - 30 kg/m2
Inclusion Criteria for the chronic tendinopathy group:
-
Debut of symptom > 90 days prior to inclusion.
-
Subjective activity related pain in patella tendon.
-
Clinical symptoms of patella tendinopathy.
-
Ultrasound verification of at least 1 in three of the following: Enchanced anterior-posterior diameter, increased Doppler signal and/or a hypoechoic area in the diseased part of the tendon.
Exclusion Criteria for all participants:
-
Former knee surgery or injuries to the patella tendon.
-
Local injection of corticosteroids within 12 months.
-
Medication that affects protein synthesis in tendon tissue.
-
Former/current use of anabolic steroids or growth hormone.
-
Smoking
-
Known rheumatoid disease or diabetes.
-
Former participation in trials using deuterated water and 15N tracers.
-
Treatment for patella tendinopathy within 3 months.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Institute of Sports Medicine Copenhagen | Copenhagen | Capital Region | Denmark | 2400 |
Sponsors and Collaborators
- Bispebjerg Hospital
Investigators
- Principal Investigator: Michael Kjær, Professor, Institute of Sports Medicine, Copenhagen
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- Project 159